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Synonyms
-
Plasmid
pGEX6P NleL S170-R782(end)
Parent Plasmid
pGEX6P1
Expressed
GST NleL S170-R782(end)
DU Number
DU45299
Genbank
Codon optimised from NC_002695 REGION: 1579163..1581511
Tag
GST
Sequence of Insert
View Sequence
GGATCCAGCCAGGGCCGCGCGTGCCTGAGCAAAGCGGAACTGACCGCGGATCTGATTTGGCTGAGCGCGAACCGCACCGGCGAAGAAAGCGCGGAAGAACTGAACTATAGCGGCTGCGATCTGAGCGGCCTGAGCCTGGTGGGCCTGAACCTGAGCAGCGTGAACTTTAGCGGCGCGGTGCTGGATGATACCGATCTGCGCATGAGCGATCTGAGCCAGGCGGTGCTGGAAAACTGCAGCTTTAAAAACAGCATTCTGAACGAATGCAACTTTTGCTATGCGAACCTGAGCAACTGCATTATTCGCGCGCTGTTTGAAAACAGCAACTTTAGCAACAGCAACCTGAAAAACGCGAGCTTTAAAGGCAGCAGCTATATTCAGTATCCGCCGATTCTGAACGAAGCGGATCTGACCGGCGCGATTATTATTCCGGGCATGGTGCTGAGCGGCGCGATTCTGGGCGATGTGAAAGAACTGTTTAGCGAAAAAAGCAACACCATTAACCTGGGCGGCTGCTATATTGATCTGAGCGATATTCAGGAAAACATTCTGAGCGTGCTGGATAACTATACCAAAAGCAACAAAAGCATTCTGCTGACCATGAACACCAGCGATGATAAATATAACCATGATAAAGTGCGCGCGGCGGAAGAACTGATTAAAAAAATTAGCCTGGATGAACTGGCGGCGTTTCGCCCGTATGTGAAAATGAGCCTGGCGGATAGCTTTAGCATTCATCCGTATCTGAACAACGCGAACATTCAGCAGTGGCTGGAACCGATTTGCGATGATTTTTTTGATACCATTATGAGCTGGTTTAACAACAGCATTATGATGTATATGGAAAACGGCAGCCTGCTGCAGGCGGGCATGTATTTTGAACGCCATCCGGGCGCGATGGTGAGCTATAACAGCAGCTTTATTCAGATTGTGATGAACGGCAGCCGCCGCGATGGCATGCAGGAACGCTTTCGCGAACTGTATGAAGTGTATCTGAAAAACGAAAAAGTGTATCCGGTGACCCAGCAGAGCGATTTTGGCCTGTGCGATGGCAGCGGCAAACCGGATTGGGATGATGATAGCGATCTGGCGTATAACTGGGTGCTGCTGAGCAGCCAGGATGATGGCATGGCGATGATGTGCAGCCTGAGCCATATGGTGGATATGCTGAGCCCGAACACCAGCACCAACTGGATGAGCTTTTTTCTGTATAAAGATGGCGAAGTGCAGAACACCTTTGGCTATAGCCTGAGCAACCTGTTTAGCGAAAGCTTTCCGATTTTTAGCATTCCGTATCATAAAGCGTTTAGCCAGAACTTTGTGAGCGGCATTCTGGATATTCTGATTAGCGATAACGAACTGAAAGAACGCTTTATTGAAGCGCTGAACAGCAACAAAAGCGATTATAAAATGATTGCGGATGATCAGCAGCGCAAACTGGCGTGCGTGTGGAACCCGTTTCTGGATGGCTGGGAACTGAACGCGCAGCATGTGGATATGATTATGGGCAGCCATGTGCTGAAAGATATGCCGCTGCGCAAACAGGCGGAAATTCTGTTTTGCCTGGGCGGCGTGTTTTGCAAATATAGCAGCAGCGATATGTTTGGCACCGAATATGATAGCCCGGAAATTCTGCGCCGCTATGCGAACGGCCTGATTGAACAGGCGTATAAAACCGATCCGCAGGTGTTTGGCAGCGTGTATTATTATAACGATATTCTGGATCGCCTGCAGGGCCGCAACAACGTGTTTACCTGCACCGCGGTGCTGACCGATATGCTGACCGAACATGCGAAAGAAAGCTTTCCGGAAATTTTTAGCCTGTATTATCCGGTGGCGTGGCGCTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSQGRACLSKAELTADLIWL
SANRTGEESAEELNYSGCDLSGLSLVGLNLSSVNFSGAVLDDTDLRMSDL
SQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSNFSNSNLKNASFK
GSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTINLGG
CYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIK
KISLDELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMS
WFNNSIMMYMENGSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQ
ERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSDLAYNWVLLS
SQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGYSLSNLF
SESFPIFSIPYHKAFSQNFVSGILDILISDNELKERFIEALNSNKSDYKM
IADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFC
LGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYN
DILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSQGRACLSKAELTADLIWL
SANRTGEESAEELNYSGCDLSGLSLVGLNLSSVNFSGAVLDDTDLRMSDL
SQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSNFSNSNLKNASFK
GSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTINLGG
CYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIK
KISLDELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMS
WFNNSIMMYMENGSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQ
ERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSDLAYNWVLLS
SQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGYSLSNLF
SESFPIFSIPYHKAFSQNFVSGILDILISDNELKERFIEALNSNKSDYKM
IADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFC
LGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYN
DILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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