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UBE2E2

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Plasmid
pGEX6P-1-UBE2E2
Parent Plasmid
pGEX6P1
Expressed
GST-UBE2E2
DU Number
DU20060
Genbank
NP_689866.1
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGTCCACTGAGGCACAAAGAGTTGATGACAGTCCAAGCACTAG
TGGAGGAAGTTCCGATGGAGATCAACGTGAAAGTGTTCAGCAAGAACCAG
AAAGAGAACAAGTTCAGCCCAAGAAAAAGGAGGGAAAAATATCCAGCAAA
ACCGCTGCTAAATTGTCAACTAGTGCTAAAAGAATTCAGAAGGAACTTGC
AGAAATCACATTGGACCCTCCTCCCAACTGTAGTGCTGGACCCAAAGGAG
ACAACATTTATGAATGGAGGTCAACTATATTGGGACCCCCAGGATCTGTC
TATGAAGGAGGGGTGTTCTTTCTTGACATTACCTTTTCACCAGACTATCC
GTTTAAACCCCCTAAGGTTACCTTCCGAACAAGAATCTATCACTGTAATA
TTAACAGCCAAGGTGTGATCTGTCTGGACATCTTAAAGGACAACTGGAGT
CCGGCTTTAACTATTTCTAAAGTTCTCCTCTCCATCTGCTCACTTCTTAC
AGATTGCAACCCTGCTGACCCTCTGGTGGGCAGCATCGCCACACAGTACA
TGACCAACAGAGCAGAGCATGACCGGATGGCCAGACAGTGGACCAAGCGG
TACGCCACATAGGCGGCCGC

Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSTEAQRVDDSPSTSGGSS
DGDQRESVQQEPEREQVQPKKKEGKISSKTAAKLSTSAKRIQKELAEITL
DPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPP
KVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNP
ADPLVGSIATQYMTNRAEHDRMARQWTKRYAT*

Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial expression construct for UBE2E2 with N-terminal GST tag (contains preScission protease site)

Estimated MW of expressed protein : 49080 Daltons
100042 R 1000 1001 1047 19007
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