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UBE2U

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Plasmid
pET156P-1-UBE2U
Parent Plasmid
pET15b 6His 3C
Expressed
6His-UBE2U
DU Number
DU20061
Genbank
Q5VVX9
Tag
6His
Species
Human
Sequence of Insert
GGATCCATGCACGGCAGAGCTTACCTCTTGCTGCACAGAGACTTCTGTGA
TCTCAAGGAGAACAATTATAAGGGTATCACTGCTAAGCCTGTAAGTGAAG
ATATGATGGAATGGGAAGTTGAAATTGAAGGTCTACAGAATTCAGTTTGG
CAGGGTTTAGTCTTCCAACTGACAATACATTTTACATCGGAGTACAACTA
TGCTCCTCCAGTTGTGAAATTTATAACAATTCCGTTTCATCCAAATGTAG
ACCCACACACTGGTCAGCCCTGTATAGACTTTTTGGACAACCCTGAGAAG
TGGAATACAAACTATACATTGAGCAGCATCTTACTTGCCCTACAGGTTAT
GCTTTCTAATCCAGTGCTAGAGAATCCAGTGAATTTGGAAGCAGCCAGAA
TACTGGTTAAAGATGAATCTCTGTACAGAACAATTCTAAGACTTTTCAAC
AGGCCATTACAAATGAAAGATGACAGCCAGGAGTTACCTAAAGACCCACG
TAAATGTATCAGACCAATTAAAACAACCTCATTTAGTGATTACTACCAGA
CATGGTCCAGAATAGCTACATCAAAAGCCACAGAATACTACAGAACTCCA
TTGCTCAAAGTTCCAAATTTCATTGGACAGTATTACAAATGGAAGAAAAT
GGATCTACAGCATCAGAAAGAATGGAATTTAAAGTATTCCGTTATCAAGT
GTTGGCTTGCTAGAAAAAGAATGCCTCATGAAGTCACTCACTCAATGGAA
GAAATTAAGCTCTGCCCAACTCTAATTCCGACCACAGATGAAATTTTTCT
TGAGTCACCAACTGCAATAAATAGCATCACAGACATTTATGAAACAGAAG
AGGAGGGGTGGAAGAGTGACACTTCATTATATGAAAATGACACAGATGAG
CCCAGGGAAGAGGAAGTGGAAGATCTGATCTCCTGGACCAATACTCTCAA
TACAAATACTTCAGAAGATTAAGCGGCCGC
Amino Acid Sequence
MGSSHHHHHHSSGLEVLFQGPGSMHGRAYLLLHRDFCDLKENNYKGITAK
PVSEDMMEWEVEIEGLQNSVWQGLVFQLTIHFTSEYNYAPPVVKFITIPF
HPNVDPHTGQPCIDFLDNPEKWNTNYTLSSILLALQVMLSNPVLENPVNL
EAARILVKDESLYRTILRLFNRPLQMKDDSQELPKDPRKCIRPIKTTSFS
DYYQTWSRIATSKATEYYRTPLLKVPNFIGQYYKWKKMDLQHQKEWNLKY
SVIKCWLARKRMPHEVTHSMEEIKLCPTLIPTTDEIFLESPTAINSITDI
YETEEEGWKSDTSLYENDTDEPREEEVEDLISWTNTLNTNTSED*


Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial expression plasmid to express UBE2U with N-terminal 6HIS tag (contains preScission protease site)

Estimated MW of expressed protein : 40186 Daltons
100042 R 1000 1001 1047 19007
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