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TRIM27

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Plasmid
p6His.Mal TRIM27
Parent Plasmid
p6His.Mal6P2
Expressed
6His.MBP-TRIM27
DU Number
DU20458
Tag
MBP
Species
Human
Sequence of Insert
GGATCCATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCAGGAGACCAC
CTGCCCCGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTCGACTGCG
GCCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAG
ACTAACGTGTCGTGCCCGCAGTGCCGGGAGACCTTCCCGCAGAGGCACAT
GCGGCCCAACCGGCACCTGGCCAACGTGACCCAACTGGTAAAGCAGCTGC
GCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGGGCGTGTGCGAGAAG
CACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTG
CGTGGTGTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGC
TCGAGGAGGCGGTGGAGGGCTTCAAGGAGCAAATCCAGAACCAGCTCGAC
CATTTAAAAAGAGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGGGGGA
ACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGATGGAGAGGGAGAAGA
TTGTTTGGGAGTTTGAGCAGCTGTATCACTCCTTAAAGGAGCATGAGTAT
CGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCCATCTACAATAGCAT
CAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCC
TGATCGCTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTG
CAGGACATTGGGGACACATTGAGCAGGGCTGAAAGAATCAGGATTCCTGA
ACCTTGGATCACACCTCCAGATTTGCAAGAGAAAATCCACATTTTTGCCC
AAAAATGTCTATTCTTGACGGAGAGTCTAAAGCAGTTCACAGAAAAAATG
CAGTCAGATATGGAGAAAATCCAAGAATTAAGAGAGGCTCAGTTATACTC
AGTGGACGTGACTCTGGACCCAGACACGGCCTACCCCAGCCTGATCCTCT
CTGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCT
GACAACCCCGAGAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATG
CTTCATCGCCGGGAGACATTATTGGGAGGTAGAGGTGGGAGATAAAGCCA
AGTGGACCATAGGTGTCTGTGAAGACTCAGTGTGCAGAAAAGGTGGAGTA
ACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCTTTGTGGTATGGGAA
AGAATATTGGGCTCTTACCTCCCCAATGACTGCCCTACCCCTGCGGACCC
CGCTCCAGCGGGTGGGGATTTTCTTGGACTATGATGCTGGTGAGGTCTCC
TTCTACAACGTGACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTAC
CTTTTGTGGGCCTGTCCGGCCCTACTTCAGTCTGAGTTACTCGGGAGGGA
AAAGTGCAGCTCCTCTGATCATCTGCCCCATGAGTGGGATAGATGGGTTT
TCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTCCCCTTGAGC
GGCCGC
Amino Acid Sequence
MHHHHHHKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFG
GYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYN
KDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYA
FKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFN
KGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINA
ASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIA
ATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNS
SSNNNNNNNNNNLGLEVLFQGPLGSMASGSVAECLQQETTCPVCLQYFAE
PMMLDCGHNICCACLARCWGTAETNVSCPQCRETFPQRHMRPNRHLANVT
QLVKQLRTERPSGPGGEMGVCEKHREPLKLYCEEDQMPICVVCDRSREHR
GHSVLPLEEAVEGFKEQIQNQLDHLKRVKDLKKRRRAQGEQARAELLSLT
QMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCN
ISHLSSLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQE
KIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTA
YPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEV
EVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMT
ALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFS
LSYSGGKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Construct for bacterial expression of N-terminally 6HIS.MBP tagged TRIM27 (with Precission site)

Estimated MW of expressed protein : 105012 Daltons
100042 R 1000 1001 1047 19007
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