TRIM27

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Plasmid
pET28a-6His-DAC C3 TRIM27
Parent Plasmid
pET28a 6His thr DAC 3C
Expressed
6His.DAC C3 TRIM27
DU Number
DU20187
Genbank
NP_006501.1
Tag
DAC
Species
Human
Sequence of Insert
GGATCCATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCAGGAGACCAC
CTGCCCCGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTCGACTGCG
GCCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAG
ACTAACGTGTCGTGCCCGCAGTGCCGGGAGACCTTCCCGCAGAGGCACAT
GCGGCCCAACCGGCACCTGGCCAACGTGACCCAACTGGTAAAGCAGCTGC
GCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGGGCGTGTGCGAGAAG
CACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTG
CGTGGTGTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGC
TCGAGGAGGCGGTGGAGGGCTTCAAGGAGCAAATCCAGAACCAGCTCGAC
CATTTAAAAAGAGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGGGGGA
ACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGATGGAGAGGGAGAAGA
TTGTTTGGGAGTTTGAGCAGCTGTATCACTCCTTAAAGGAGCATGAGTAT
CGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCCATCTACAATAGCAT
CAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCC
TGATCGCTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTG
CAGGACATTGGGGACACATTGAGCAGGGCTGAAAGAATCAGGATTCCTGA
ACCTTGGATCACACCTCCAGATTTGCAAGAGAAAATCCACATTTTTGCCC
AAAAATGTCTATTCTTGACGGAGAGTCTAAAGCAGTTCACAGAAAAAATG
CAGTCAGATATGGAGAAAATCCAAGAATTAAGAGAGGCTCAGTTATACTC
AGTGGACGTGACTCTGGACCCAGACACGGCCTACCCCAGCCTGATCCTCT
CTGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCT
GACAACCCCGAGAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATG
CTTCATCGCCGGGAGACATTATTGGGAGGTAGAGGTGGGAGATAAAGCCA
AGTGGACCATAGGTGTCTGTGAAGACTCAGTGTGCAGAAAAGGTGGAGTA
ACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCTTTGTGGTATGGGAA
AGAATATTGGGCTCTTACCTCCCCAATGACTGCCCTACCCCTGCGGACCC
CGCTCCAGCGGGTGGGGATTTTCTTGGACTATGATGCTGGTGAGGTCTCC
TTCTACAACGTGACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTAC
CTTTTGTGGGCCTGTCCGGCCCTACTTCAGTCTGAGTTACTCGGGAGGGA
AAAGTGCAGCTCCTCTGATCATCTGCCCCATGAGTGGGATAGATGGGTTT
TCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTCCCCTTGAGC
GGCCGC
Amino Acid Sequence
MGSSHHHHHHSSGLVPRGSHMIPGVPQIDAESYILIDYNSGKVLAEQNAD
VRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSS
LMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYV
NALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKE
FTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLIS
AVMGGRTFKGREAESKKLLTWGFRFFETVNPLEVLFQGPGGSMASGSVAE
CLQQETTCPVCLQYFAEPMMLDCGHNICCACLARCWGTAETNVSCPQCRE
TFPQRHMRPNRHLANVTQLVKQLRTERPSGPGGEMGVCEKHREPLKLYCE
EDQMPICVVCDRSREHRGHSVLPLEEAVEGFKEQIQNQLDHLKRVKDLKK
RRRAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELD
LAIYNSINGAITQFSCNISHLSSLIAQLEEKQQQPTRELLQDIGDTLSRA
ERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQEL
REAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFP
CVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWA
VSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHT
FTFSHATFCGPVRPYFSLSYSGGKSAAPLIICPMSGIDGFSGHVGNHGHS
METSP*



Vector Type
Bacterial
Comments
Bacterial expression vector for TRIM27 full-length with N-terminal 6HIS-DAC tag (cleavage sites as follows: 6HIS-thrombin–DAC-Precission protease)
Parental PLasmid 21713

Estimated MW of expressed protein : 90198 Daltons
100042 R 1000 1001 1047 19007
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