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KLHL3

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Plasmid
pCMV-FLAG-1 KLHL3 A77E
Parent Plasmid
pCMV5 FLAG1
Expressed
FLAG-KLHL3 A77E
DU Number
DU23144
Genbank
NM_017415.2
Tag
FLAG
Species
Human
Sequence of Insert
AGATCTatggagggtgaaagtgtcaagctgagctcccagactctgataca
ggctggggatgatgagaagaaccagaggacgatcactgtcaaccctgccc
acatggggaaagcattcaaggttatgaatgaactgcggagtaaacagctg
ttgtgtgacgtgatgattgtggcagaagatgtcgagatagaagcccaccg
tgtggtcctggcagcctgcagcccctacttctgtgAgatgttcacaggtg
acatgtctgagagtaaagccaaaaagatagaaatcaaggacgtggatggg
cagacgctgagtaagctgattgactacatctatactgctgaaatcgaggt
gactgaagagaatgtccaggtgctgctcccggcagccagcttgctgcagc
tcatggatgttcggcagaactgctgtgacttcctgcagtctcagttgcat
cccaccaattgcctgggcatccgtgcgtttgcagatgtacacacctgcac
tgaccttctgcagcaggccaatgcctacgcagagcagcactttccagagg
tgatgctaggagaagaatttcttagcctgagtctggaccaggtgtgcagc
ttgatatccagcgacaagctgaccgtttcttcagaagagaaggtgtttga
agctgtgatctcatggatcaattatgagaaagaaacccgtttagagcaca
tggcaaagctgatggaacatgtccgacttcctctcttacctagggactac
ctagtccaaacggttgaagaagaagctttgataaagaataacaacacctg
taaagacttcctcattgaggccatgaaataccatctcctccctctggatc
agagactattgattaagaacccaaggaccaagcccaggactccagtcagc
cttcccaaggtcatgattgtggttggcggccaggcacccaaggcaatccg
cagtgtggagtgctatgatttcgaggaggaccggtgggatcagattgctg
agcttccttccagaagatgcagagcaggtgtggtgttcatggctggccac
gtgtatgccgtgggagggtttaatggctcactgcgggtgcggacagtgga
tgtgtatgacggcgtgaaggaccagtggacgtccattgccagcatgcagg
agcgccggagcacactgggcgcagcggtgctcaatgacttgctctacgca
gtgggaggctttgatggcagtactggcctagcatcggtggaagcctacag
ctacaagaccaacgagtggttctttgtggccccgatgaacacgcggcgga
gcagtgtgggtgtgggcgttgtggaggggaagctatatgctgttgggggt
tatgatggagcttcccgccagtgtctgagcactgtggagcagtacaaccc
agcgaccaatgaatggatatacgtggcggacatgagcacccgccgcagtg
gcgcaggggttggagtgcttagcggacagctgtacgccacaggtgggcat
gatgggcctttggtgaggaagagcgttgaggtttacgatcctggaacaaa
tacctggaagcaagtggcagacatgaacatgtgccggcgcaacgcagggg
tctgtgcagtaaatgggctcctgtatgtggttggaggggatgatggatcc
tgcaacttggcttcggtggagtactacaatcctgtcactgacaaatggac
gctgcttccaacgaacatgagcacggggcggagctatgcaggtgttgccg
tgattcacaagtccttgtgaGCGGCCGC
Amino Acid Sequence
MDYKDDDDKGSMEGESVKLSSQTLIQAGDDEKNQRTITVNPAHMGKAFKV
MNELRSKQLLCDVMIVAEDVEIEAHRVVLAACSPYFCEMFTGDMSESKAK
KIEIKDVDGQTLSKLIDYIYTAEIEVTEENVQVLLPAASLLQLMDVRQNC
CDFLQSQLHPTNCLGIRAFADVHTCTDLLQQANAYAEQHFPEVMLGEEFL
SLSLDQVCSLISSDKLTVSSEEKVFEAVISWINYEKETRLEHMAKLMEHV
RLPLLPRDYLVQTVEEEALIKNNNTCKDFLIEAMKYHLLPLDQRLLIKNP
RTKPRTPVSLPKVMIVVGGQAPKAIRSVECYDFEEDRWDQIAELPSRRCR
AGVVFMAGHVYAVGGFNGSLRVRTVDVYDGVKDQWTSIASMQERRSTLGA
AVLNDLLYAVGGFDGSTGLASVEAYSYKTNEWFFVAPMNTRRSSVGVGVV
EGKLYAVGGYDGASRQCLSTVEQYNPATNEWIYVADMSTRRSGAGVGVLS
GQLYATGGHDGPLVRKSVEVYDPGTNTWKQVADMNMCRRNAGVCAVNGLL
YVVGGDDGSCNLASVEYYNPVTDKWTLLPTNMSTGRSYAGVAVIHKSL*
Vector Type
Mammalian
Antibiotic
Amp
Comments
clone harbours silent a471g substitution (Ala157)
100042 R 1000 1001 1047 19007
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