Ubiquitin

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Plasmid
pET156P-Ub1 FRET donor
Parent Plasmid
pET15b 6His 3C
Expressed
6His-LBT-Ubiquitin K48R K63R
DU Number
DU20516
Genbank
P62988
Tag
6His
Species
Human
Sequence of Insert
GGATCCATGGCGTGCGCGGATTATAACAAAGATGGCTGGTATGAAGAACT
GGAGTGCGCGGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCA
TCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAG
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC
TGGGAGACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGA
GAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGAGCGGCC
GC
Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMACADYNKDGWYEELECAAMQIFVKTL
TGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGRQLEDGRTLSD
YNIQRESTLHLVLRLRGG*



Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial expression construct to express 6HIS-LBT(Lanthanide binding peptide)-Ubiquitin K48R K63R (contains preScission protease site)

Estimated MW of expressed protein : 13230 Daltons
100042 R 1000 1001 1047 19007
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