UBE2T | Ubiquitylation Resource

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Plasmid

pcDNA5-FRT/TO-GFP UBE2T

Parent Plasmid

pcDNA5 FRT/TO GFP

Expressed

GFP-UBE2T

DU Number

DU20050

Genbank

NP_054895.1

Tag

GFP

Species

Human

Sequence of Insert

View Sequence

GGATCCATGCAGAGAGCTTCACGTCTGAAGAGAGAGCTGCACATGTTAGC
CACAGAGCCACCCCCAGGCATCACATGTTGGCAAGATAAAGACCAAATGG
ATGACCTGCGAGCTCAAATATTAGGTGGAGCCAACACACCTTATGAGAAA
GGTGTTTTTAAGCTAGAAGTTATCATTCCTGAGAGGTACCCATTTGAACC
TCCTCAGATCCGATTTCTCACTCCAATTTATCATCCAAACATTGATTCTG
CTGGAAGGATTTGTCTGGATGTTCTCAAATTGCCACCAAAAGGTGCTTGG
AGACCATCCCTCAACATCGCAACTGTGTTGACCTCTATTCAGCTGCTCAT
GTCAGAACCCAACCCTGATGACCCGCTCATGGCTGACATATCCTCAGAAT
TTAAATATAATAAGCCAGCCTTCCTCAAGAATGCCAGACAGTGGACAGAG
AAGCATGCAAGACAGAAACAAAAGGCTGATGAGGAAGAGATGCTTGATAA
TCTACCAGAGGCTGGTGACTCCAGAGTACACAACTCAACACAGAAAAGGA
AGGCCAGTCAGCTAGTAGGCATAGAAAAGAAATTTCATCCTGATGTTTAG
GCGGCCGC

Amino Acid Sequence

View Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMQRASR
LKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVI
IPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIAT
VLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQK
ADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDV

Vector Type

Mammalian

Antibiotic

Amp

Comments

N terminal GFP for mammalian expression Flp-In vector.

Estimated MW of expressed protein : 49846 Daltons

100042 R 1000 1001 1047 19007

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