UBE2Q | Ubiquitylation Resource

Synonyms

-

Plasmid

pET28-UBE2Q

Parent Plasmid

pET28a original

Expressed

6His-UBE2Q

DU Number

DU20176

Genbank

NP_060052

Tag

6His

Species

Human

Sequence of Insert

View Sequence

GAATTCCAGCAGCCGCAGCCGCAGGGGCAGCAGCAGCCGGGGCCGGGGCA
GCAGCTGGGGGGCCAGGGGGCGGCGCCGGGGGCCGGGGGCGGCCCAGGGG
GGGGCCCGGGGCCGGGGCCCTGCCTGAGGCGAGAGCTGAAGCTGCTCGAG
TCCATCTTCCACCGCGGCCACGAGCGCTTCCGCATTGCCAGCGCCTGCCT
GGACGAGCTGAGCTGCGAGTTCCTGCTGGCTGGGGCCGGAGGGGCCGGGG
CGGGGGCCGCGCCCGGACCGCATCTCCCCCCACGGGGGTCGGTGCCTGGG
GATCCTGTCCGCATCCACTGCAACATCACGGAGTCATACCCTGCTGTGCC
CCCCATCTGGTCGGTGGAGTCTGATGACCCTAACTTGGCTGCTGTCTTGG
AGAGGCTGGTGGACATAAAGAAAGGGAATACTCTGCTATTGCAGCATCTG
AAGAGGATCATCTCCGACCTGTGTAAACTCTATAACCTCCCTCAGCATCC
AGATGTGGAGATGCTGGATCAACCCTTGCCAGCAGAGCAGTGCACACAGG
AAGACGTGTCTTCAGAAGATGAAGATGAGGAGATGCCTGAGGACACAGAA
GACTTAGATCACTATGAAATGAAAGAGGAAGAGCCAGCTGAGGGCAAGAA
ATCTGAAGATGATGGCATTGGAAAAGAAAACTTGGCCATCCTAGAGAAAA
TTAAAAAGAACCAGAGGCAAGATTACTTAAATGGTGCAGTGTCTGGCTCG
GTGCAGGCCACTGACCGGCTGATGAAGGAGCTCAGGGATATATACCGATC
ACAGAGTTTCAAAGGCGGAAACTATGCAGTCGAACTCGTGAATGACAGTC
TGTATGATTGGAATGTCAAACTCCTCAAAGTTGACCAGGACAGCGCTTTG
CACAACGATCTCCAGATCCTCAAAGAGAAAGAAGGAGCCGACTTCATTCT
ACTTAACTTTTCCTTTAAAGATAACTTTCCCTTTGACCCACCATTTGTCA
GGGTTGTGTCTCCAGTCCTCTCTGGAGGGTATGTTCTGGGCGGAGGGGCC
ATCTGCATGGAACTTCTCACCAAACAGGGCTGGAGCAGTGCCTACTCCAT
AGAGTCAGTGATCATGCAGATCAGTGCCACACTGGTGAAGGGGAAAGCAC
GAGTGCAGTTTGGAGCCAACAAATCTCAATACAGTCTGACAAGAGCACAG
CAGTCCTACAAGTCCTTGGTGCAGATCCACGAAAAAAACGGCTGGTACAC
ACCCCCAAAAGAAGACGGCTAAGCGGCCGC

Amino Acid Sequence

View Sequence

HHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFQQPQPQGQQQPGPGQQLG
GQGAAPGAGGGPGGGPGPGPCLRRELKLLESIFHRGHERFRIASACLDEL
SCEFLLAGAGGAGAGAAPGPHLPPRGSVPGDPVRIHCNITESYPAVPPIW
SVESDDPNLAAVLERLVDIKKGNTLLLQHLKRIISDLCKLYNLPQHPDVE
MLDQPLPAEQCTQEDVSSEDEDEEMPEDTEDLDHYEMKEEEPAEGKKSED
DGIGKENLAILEKIKKNQRQDYLNGAVSGSVQATDRLMKELRDIYRSQSF
KGGNYAVELVNDSLYDWNVKLLKVDQDSALHNDLQILKEKEGADFILLNF
SFKDNFPFDPPFVRVVSPVLSGGYVLGGGAICMELLTKQGWSSAYSIESV
IMQISATLVKGKARVQFGANKSQYSLTRAQQSYKSLVQIHEKNGWYTPPK
EDG*

Vector Type

Bacterial

Comments

Bacterial expression construct to express UBE2Q with N-terminal 6HIS tag (contains Thrombin cleavage site)

Estimated MW of expressed protein : 49454 Daltons

100042 R 1000 1001 1047 19007