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Plasmid
pCMV5-FLAG-1 Optineurin E478A
Parent Plasmid
pCMV5
Expressed
FLAG-Optineurin E478A
DU Number
DU4652
Genbank
AF420371
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
GCCACCATGGACTACAAGGACGATGACGATAAGGGATCCATGTCCCATCA
ACCTCTCAGCTGCCTCACTGAAAAGGAGGACAGCCCCAGTGAAAGCACAG
GAAATGGACCCCCCCACCTGGCCCACCCAAACCTGGACACGTTTACCCCG
GAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTGACCGAGAACCACCAGCT
GAAAGAAGCCATGAAGCTAAATAATCAAGCCATGAAAGGGAGATTTGAGG
AGCTTTCGGCCTGGACAGAGAAACAGAAGGAAGAACGCCAGTTTTTTGAG
ATACAGAGCAAAGAAGCAAAAGAGCGTCTAATGGCCTTGAGTCATGAGAA
TGAGAAATTGAAGGAAGAGCTTGGAAAACTAAAAGGGAAATCAGAAAGGT
CATCTGAGGACCCCACTGATGACTCCAGGCTTCCCAGGGCCGAAGCGGAG
CAGGAAAAGGACCAGCTCAGGACCCAGGTGGTGAGGCTACAAGCAGAGAA
GGCAGACCTGTTGGGCATCGTGTCTGAACTGCAGCTCAAGCTGAACTCCA
GCGGCTCCTCAGAAGATTCCTTTGTTGAAATTAGGATGGCTGAAGGAGAA
GCAGAAGGGTCAGTAAAAGAAATCAAGCATAGTCCTGGGCCCACGAGAAC
AGTCTCCACTGGCACGGCATTGTCTAAATATAGGAGCAGATCTGCAGATG
GGGCCAAGAATTACTTCGAACATGAGGAGTTAACTGTGAGCCAGCTCCTG
CTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAGAGACTTGAAGTTGCACT
CAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGAAAAGAAAACAAGTAATC
GTTCTGAGATTGAAACCCAGACAGAGGGGAGCACAGAGAAAGAGAATGAT
GAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAAGTGGAAGCACTGAACCT
CCAGGTGACATCTCTGTTTAAGGAGCTTCAAGAGGCTCATACAAAACTCA
GCGAAGCTGAGCTAATGAAGAAGAGACTTCAAGAAAAGTGTCAGGCCCTT
GAAAGGAAAAATTCTGCAATTCCATCAGAGTTGAATGAAAAGCAAGAGCT
TGTTTATACTAACAAAAAGTTAGAGCTACAAGTGGAAAGCATGCTATCAG
AAATCAAAATGGAACAGGCTAAAACAGAGGATGAAAAGTCCAAATTAACT
GTGCTACAGATGACACACAACAAGCTTCTTCAAGAACATAATAATGCATT
GAAAACAATTGAGGAACTAACAAGAAAAGAGTCAGAAAAAGTGGACAGGG
CAGTGCTGAAGGAACTGAGTGAAAAACTGGAACTGGCAGAGAAGGCTCTG
GCTTCCAAACAGCTGCAAATGGATGAAATGAAGCAAACCATTGCCAAGCA
GGAAGAGGACCTGGAAACCATGACCATCCTCAGGGCTCAGATGGAAGTTT
ACTGTTCTGATTTTCATGCTGCAAGAGCAGCGAGAGAGAAAATTCATGAG
GAAAAGGAGCAACTGGCATTGCAGCTGGCAGTTCTGCTGAAAGAGAATGA
TGCTTTCGAAGACGGAGGCAGGCAGTCCTTGATGGAGATGCAGAGTCGTC
ATGGGGCGAGAACAAGTGACTCTGACCAGCAGGCTTACCTTGTTCAAAGA
GGAGCTGAGGACAGGGACTGGCGGCAACAGCGGAATATTCCGATTCATTC
CTGCCCCAAGTGTGGAGAGGTTCTGCCTGACATAGACACGTTACAGATTC
ACGTGATGGATTGCATCATTTAAGCGGCCGCG
ACCTCTCAGCTGCCTCACTGAAAAGGAGGACAGCCCCAGTGAAAGCACAG
GAAATGGACCCCCCCACCTGGCCCACCCAAACCTGGACACGTTTACCCCG
GAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTGACCGAGAACCACCAGCT
GAAAGAAGCCATGAAGCTAAATAATCAAGCCATGAAAGGGAGATTTGAGG
AGCTTTCGGCCTGGACAGAGAAACAGAAGGAAGAACGCCAGTTTTTTGAG
ATACAGAGCAAAGAAGCAAAAGAGCGTCTAATGGCCTTGAGTCATGAGAA
TGAGAAATTGAAGGAAGAGCTTGGAAAACTAAAAGGGAAATCAGAAAGGT
CATCTGAGGACCCCACTGATGACTCCAGGCTTCCCAGGGCCGAAGCGGAG
CAGGAAAAGGACCAGCTCAGGACCCAGGTGGTGAGGCTACAAGCAGAGAA
GGCAGACCTGTTGGGCATCGTGTCTGAACTGCAGCTCAAGCTGAACTCCA
GCGGCTCCTCAGAAGATTCCTTTGTTGAAATTAGGATGGCTGAAGGAGAA
GCAGAAGGGTCAGTAAAAGAAATCAAGCATAGTCCTGGGCCCACGAGAAC
AGTCTCCACTGGCACGGCATTGTCTAAATATAGGAGCAGATCTGCAGATG
GGGCCAAGAATTACTTCGAACATGAGGAGTTAACTGTGAGCCAGCTCCTG
CTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAGAGACTTGAAGTTGCACT
CAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGAAAAGAAAACAAGTAATC
GTTCTGAGATTGAAACCCAGACAGAGGGGAGCACAGAGAAAGAGAATGAT
GAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAAGTGGAAGCACTGAACCT
CCAGGTGACATCTCTGTTTAAGGAGCTTCAAGAGGCTCATACAAAACTCA
GCGAAGCTGAGCTAATGAAGAAGAGACTTCAAGAAAAGTGTCAGGCCCTT
GAAAGGAAAAATTCTGCAATTCCATCAGAGTTGAATGAAAAGCAAGAGCT
TGTTTATACTAACAAAAAGTTAGAGCTACAAGTGGAAAGCATGCTATCAG
AAATCAAAATGGAACAGGCTAAAACAGAGGATGAAAAGTCCAAATTAACT
GTGCTACAGATGACACACAACAAGCTTCTTCAAGAACATAATAATGCATT
GAAAACAATTGAGGAACTAACAAGAAAAGAGTCAGAAAAAGTGGACAGGG
CAGTGCTGAAGGAACTGAGTGAAAAACTGGAACTGGCAGAGAAGGCTCTG
GCTTCCAAACAGCTGCAAATGGATGAAATGAAGCAAACCATTGCCAAGCA
GGAAGAGGACCTGGAAACCATGACCATCCTCAGGGCTCAGATGGAAGTTT
ACTGTTCTGATTTTCATGCTGCAAGAGCAGCGAGAGAGAAAATTCATGAG
GAAAAGGAGCAACTGGCATTGCAGCTGGCAGTTCTGCTGAAAGAGAATGA
TGCTTTCGAAGACGGAGGCAGGCAGTCCTTGATGGAGATGCAGAGTCGTC
ATGGGGCGAGAACAAGTGACTCTGACCAGCAGGCTTACCTTGTTCAAAGA
GGAGCTGAGGACAGGGACTGGCGGCAACAGCGGAATATTCCGATTCATTC
CTGCCCCAAGTGTGGAGAGGTTCTGCCTGACATAGACACGTTACAGATTC
ACGTGATGGATTGCATCATTTAAGCGGCCGCG
Amino Acid Sequence
View Sequence
MDYKDDDDKGSMSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEE
LLQQMKELLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQ
SKEAKERLMALSHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQE
KDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAE
GSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLC
LREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEE
KGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALER
KNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVL
QMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALAS
KQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAARAAREKIHEEK
EQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGA
EDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII*
LLQQMKELLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQ
SKEAKERLMALSHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQE
KDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAE
GSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLC
LREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEE
KGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALER
KNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVL
QMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALAS
KQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAARAAREKIHEEK
EQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGA
EDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Mammalian expression with N term FLAG.
100042 R 1000 1001 1047 19007
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