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UBE2J1

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Plasmid
pET28(+) UBE2J1
Parent Plasmid
pET28a original
Expressed
6H-UBE2J1
DU Number
DU14077
Genbank
NM_016021
Tag
6His
Species
Human
Sequence of Insert
GGATCCATGGAGACCCGCTACAACCTGAAGAGTCCGGCTGTTAAACGTTT
AATGAAAGAAGCGGCAGAATTGAAAGATCCAACAGATCATTACCATGCGC
AGCCTTTAGAGGATAACCTTTTTGAATGGCACTTCACGGTTAGAGGGCCC
CCAGACTCCGATTTTGATGGAGGAGTTTATCACGGGCGGATAGTACTGCC
ACCAGAGTATCCCATGAAACCACCAAGCATTATTCTCCTAACGGCTAATG
GTCGATTTGAAGTGGGCAAGAAAATCTGTTTGAGCATCTCAGGCCATCAT
CCTGAAACTTGGCAGCCTTCGTGGAGTATAAGGACAGCATTATTAGCCAT
CATTGGGTTTATGCCAACAAAAGGAGAGGGAGCCATAGGTTCTCTAGATT
ACACTCCTGAGGAAAGAAGAGCACTTGCCAAAAAATCACAAGATTTCTGT
TGTGAAGGATGTGGCTCTGCCATGAAGGATGTCCTGTTGCCTTTAAAATC
TGGAAGCGATTCAAGCCAAGCTGACCAAGAAGCCAAAGAACTGGCTAGGC
AAATAAGCTTTAAGGCAGAAGTCAATTCATCTGGAAAGACTATCTCTGAG
TCAGACTTAAACCACTCTTTTTCACTAACTGATTTACAAGATGATATACC
TACAACATTCCAGGGTGCTACGGCCAGTACATCGTACGGACTCCAGAATT
CCTCAGCAGCATCCTTTCATCAACCTACCCAACCTGTAGCTAAGAATACC
TCCATGAGCCCTCGACAGCGCCGGGCCCAGCAGCAGAGTCAGAGAAGGTT
GTCTACTTCACCAGATGTAATCCAGGGCCACCAGCCAAGAGACAACCACA
CTGATCATGGTGGGTCAGCTGTACTGATTGTCATCCTGACTTTGGCATTG
GCAGCTCTTATATTCCGACGAATATATCTGGCAAACGAATACATATTTGA
CTTTGAGTTATAAGCGGCCGC
Amino Acid Sequence
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMETRYNLKSPAVKRLM
KEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPP
EYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAII
GFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSG
SDSSQADQEAKELARQISFKAEVNSSGKTISESDLNHSFSLTDLQDDIPT
TFQGATASTSYGLQNSSAASFHQPTQPVAKNTSMSPRQRRAQQQSQRRLS
TSPDVIQGHQPRDNHTDHGGSAVLIVILTLALAALIFRRIYLANEYIFDF
EL*
Vector Type
Bacterial
Antibiotic
Kan
Comments
N ter 6HIS for bacterial expression.

Estimated MW of expressed protein : 38741 Daltons
100042 R 1000 1001 1047 19007
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