UBXD7 | Ubiquitylation Resource

Plasmid

pGEX-TEV-FLAG UBXD7 P459G

Parent Plasmid

pGEX TEV TEV

Expressed

GST-FLAG UBXD7 P459G

DU Number

DU21293

Genbank

NM_015562

Tag

GST

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCGCTGCCCACGGGGGCTCCGCGGCGTCCTCGGCGCTGAAGGGGTT
AATTCAACAGTTCACCACCATTACCGGTGCAAGTGAAAGTGTAGGAAAAC
ATATGCTTGAAGCGTGCAACAATAATCTGGAAATGGCAGTCACTATGTTT
TTGGATGGTGGAGGAATCGCTGAAGAGCCCAGTACCAGTTCAGCAAGTGT
CTCTACTGTCAGACCACACACAGAAGAAGAAGTTCGTGCCCCAATTCCTC
AAAAGCAGGAAATACTGGTGGAACCAGAACCATTATTTGGTGCTCCTAAA
AGACGACGGCCTGCACGTTCAATTTTTGATGGTTTCCGGGATTTTCAGAC
TGAAACTATTCGGCAAGAACAAGAATTAAGAAATGGAGGAGCTATCGATA
AGAAATTAACTACCCTTGCAGATCTATTCCGGCCACCCATTGATTTGATG
CATAAAGGCAGCTTTGAAACAGCCAAAGAGTGTGGCCAGATGCAAAATAA
GTGGCTGATGATAAACATTCAAAATGTTCAAGACTTTGCATGTCAGTGCC
TCAACCGCGATGTGTGGAGCAACGAAGCTGTGAAGAATATTATCCGGGAA
CATTTCATTTTCTGGCAGGTTTATCATGACAGTGAGGAAGGTCAGAGATA
CATACAGTTTTATAAGTTAGGGGATTTCCCCTATGTTTCCATATTGGACC
CACGGACAGGTCAGAAGCTAGTAGAATGGCACCAGTTAGATGTATCTTCT
TTCTTGGACCAAGTGACGGGATTTCTGGGTGAACATGGACAACTGGATGG
ACTTTCTAGCAGTCCCCCCAAAAAATGTGCCCGTTCCGAGAGCCTTATAG
ATGCAAGTGAAGACAGCCAGCTAGAAGCTGCCATCAGAGCCTCCTTACAA
GAAACACATTTTGATTCAACACAGACAAAACAGGATAGCCGCTCAGATGA
AGAATCTGAATCTGAACTTTTTTCTGGCAGTGAGGAGTTCATATCCGTTT
GTGGCTCTGATGAAGAAGAAGAGGTAGAGAATCTTGCCAAGTCCAGAAAG
TCTCCCCACAAAGATTTGGGGCATAGAAAAGAGGAGAATAGAAGGCCGCT
GACTGAGCCACCAGTCAGAACTGATCCTGGAACAGCCACAAACCACCAAG
GATTGCCAGCTGTGGATTCAGAGATACTGGAGATGCCACCTGAAAAAGCA
GATGGAGTAGTGGAGGGGATAGATGTAAATGGACCAAAAGCACAGCTGAT
GTTGCGGTATCCAGATGGAAAAAGGGAACAGATCACTCTTCCAGAGCAAG
CTAAACTGCTAGCTTTGGTGAAGCACGTGCAGTCTAAAGGATACCCAAAT
GAACGTTTTGAACTTCTCACCAACTTTGGTCGAAGGAAATTATCTCATCT
GGACTATGATATTACATTGCAAGAGGCAGGCCTTTGTCCTCAAGAGACTG
TCTTTGTACAGGAAAGAAATTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQDHPPKSDENLYFQGGSDYKDDDDKGSAAHGGSAASSALKGLIQQFTTITGASESVGKHMLEACNNNLEMAVTMFLDGGGIAEEPSTSSASVSTVRPHTEEEVRAPIPQKQEILVEPEPLFGAPKRRRPARSIFDGFRDFQTETIRQEQELRNGGAIDKKLTTLADLFRPPIDLMHKGSFETAKECGQMQNKWLMINIQNVQDFACQCLNRDVWSNEAVKNIIREHFIFWQVYHDSEEGQRYIQFYKLGDFPYVSILDPRTGQKLVEWHQLDVSSFLDQVTGFLGEHGQLDGLSSSPPKKCARSESLIDASEDSQLEAAIRASLQETHFDSTQTKQDSRSDEESESELFSGSEEFISVCGSDEEEEVENLAKSRKSPHKDLGHRKEENRRPLTEPPVRTDPGTATNHQGLPAVDSEILEMPPEKADGVVEGIDVNGPKAQLMLRYPDGKREQITLPEQAKLLALVKHVQSKGYPNERFELLTNFGRRKLSHLDYDITLQEAGLCPQETVFVQERN

Vector Type

Bacterial

Antibiotic

Amp

Comments

Differs from reference sequence in a single silent mutation only – a834c

Estimated MW of expressed protein : 84055 Datlons

100042 R 1000 1001 1047 19007