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Plasmid
pGEX6P-3-UBE2A
Parent Plasmid
pGEX6P3
Expressed
GST-UBE2A
DU Number
DU4203
Genbank
NP_003327
Tag
GST
Species
Human
Sequence of Insert
View Sequence
ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGAATT
CCCGGGTCGACTCCACCCCGGCTCGGCGGCGCCTCATGCGGGACTTCAAG
AGGTTGCAGGAGGATCCTCCAGCCGGAGTCAGCGGGGCTCCGTCCGAGAA
CAACATAATGGTGTGGAACGCGGTCATTTTCGGGCCTGAAGGGACCCCGT
TTGAGGATGGAACATTTAAACTTACAATAGAATTCACTGAAGAATATCCA
AATAAACCACCTACAGTTAGATTTGTCTCTAAGATGTTCCATCCAAATGT
CTATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTC
CAACCTATGATGTGTCTTCCATTCTAACATCCATACAGTCTCTGTTGGAT
GAACCCAATCCCAATAGTCCAGCAAACAGCCAGGCTGCTCAGCTGTACCA
GGAGAACAAACGGGAATATGAAAAGCGTGTTTCTGCAATAGTAGAACAAA
GCTGGCGTGATTGTTGAGCGGCCGC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGAATT
CCCGGGTCGACTCCACCCCGGCTCGGCGGCGCCTCATGCGGGACTTCAAG
AGGTTGCAGGAGGATCCTCCAGCCGGAGTCAGCGGGGCTCCGTCCGAGAA
CAACATAATGGTGTGGAACGCGGTCATTTTCGGGCCTGAAGGGACCCCGT
TTGAGGATGGAACATTTAAACTTACAATAGAATTCACTGAAGAATATCCA
AATAAACCACCTACAGTTAGATTTGTCTCTAAGATGTTCCATCCAAATGT
CTATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTC
CAACCTATGATGTGTCTTCCATTCTAACATCCATACAGTCTCTGTTGGAT
GAACCCAATCCCAATAGTCCAGCAAACAGCCAGGCTGCTCAGCTGTACCA
GGAGAACAAACGGGAATATGAAAAGCGTGTTTCTGCAATAGTAGAACAAA
GCTGGCGTGATTGTTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDSTPARRRLMRDFK
RLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYP
NKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLD
EPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDSTPARRRLMRDFK
RLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYP
NKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLD
EPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial expression with N terminal GST.
100042 R 1000 1001 1047 19007
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