TRAF3 | Ubiquitylation Resource

Plasmid

pFBHTb TRAF3

Parent Plasmid

pFastBac HTb

Expressed

6His-TRAF3

DU Number

DU12006

Genbank

AAH75086.1

Tag

6His

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGAGTCGAGTAAAAAGATGGACTCTCCTGGCGCGCTGCAGAC
TAACCCGCCGCTAAAGCTGCACACTGACCGCAGTGCTGGGACGCCAGTTT
TTGTCCCTGAACAAGGAGGTTACAAGGAAAAGTTTGTGAAGACCGTGGAG
GACAAGTACAAGTGTGAGAAGTGCCACCTGGTGCTGTGCAGCCCGAAGCA
GACCGAGTGTGGGCACCGCTTCTGCGAGAGCTGCATGGCGGCCCTGCTGA
GCTCTTCAAGTCCAAAATGTACAGCGTGTCAAGAGAGCATCGTTAAAGAT
AAGGTGTTTAAGGATAATTGCTGCAAGAGAGAAATTCTGGCTCTTCAGAT
CTATTGTCGGAATGAAAGCAGAGGTTGTGCAGAGCAGTTAATGCTGGGAC
ATCTGCTGGTGCATTTAAAAAATGATTGCCATTTTGAAGAACTTCCATGT
GTGCGTCCTGACTGCAAAGAAAAGGTCTTGAGGAAAGACCTGCGAGACCA
CGTGGAGAAGGCGTGTAAATACCGGGAAGCCACATGCAGCCACTGCAAGA
GTCAGGTTCCGATGATCGCGCTGCAGAAACACGAAGACACCGACTGTCCC
TGCGTGGTGGTGTCCTGCCCTCACAAGTGCAGCGTCCAGACTCTCCTGAG
GAGCGAGTTGAGTGCACACTTGTCAGAGTGTGTCAATGCCCCCAGCACCT
GTAGTTTTAAGCGCTATGGCTGCGTTTTTCAGGGGACAAACCAGCAGATC
AAGGCCCACGAGGCCAGCTCCGCCGTGCAGCACGTCAACCTGCTGAAGGA
GTGGAGCAACTCGCTCGAAAAGAAGGTTTCCTTGTTGCAGAATGAAAGTG
TAGAAAAAAACAAGAGCATACAAAGTTTGCACAATCAGATATGTAGCTTT
GAAATTGAAATTGAGAGACAAAAGGAAATGCTTCGAAATAATGAATCCAA
AATCCTTCATTTACAGCGAGTGATAGACAGCCAAGCAGAGAAACTGAAGG
AGCTTGACAAGGAGATCCGGCCCTTCCGGCAGAACTGGGAGGAAGCAGAC
AGCATGAAGAGCAGCGTGGAGTCCCTCCAGAACCGCGTGACCGAGCTGGA
GAGCGTGGACAAGAGCGCGGGGCAAGTGGCTCGGAACACAGGCCTGCTGG
AGTCCCAGCTGAGCCGGCATGACCAGATGCTGAGTGTGCACGACATCCGC
CTAGCCGACATGGACCTGCGCTTCCAGGTCCTGGAGACCGCCAGCTACAA
TGGAGTGCTCATCTGGAAGATTCGCGACTACAAGCGGCGGAAGCAGGAGG
CCGTCATGGGGAAGACCCTGTCCCTTTACAGCCAGCCTTTCTACACTGGT
TACTTTGGCTATAAGATGTGTGCCAGGGTCTACCTGAACGGGGACGGGAT
GGGGAAGGGGACGCACTTGTCGCTGTTTTTTGTCATCATGCGTGGAGAAT
ATGATGCCCTGCTTCCTTGGCCGTTTAAGCAGAAAGTGACACTCATGCTG
ATGGATCAGGGGTCCTCTCGACGTCATTTGGGAGATGCATTCAAGCCCGA
CCCCAACAGCAGCAGCTTCAAGAAGCCCACTGGAGAGATGAATATCGCCT
CTGGCTGCCCAGTCTTTGTGGCCCAAACTGTTCTAGAAAATGGGACATAT
ATTAAAGATGATACAATTTTTATTAAAGTCATAGTGGATACTTCGGATCT
GCCCGATCCCTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MSYHHHHHHDYDIPTTENLYFQGAMGSMESSKKMDSPGALQTNPPLKLHT
DRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFC
ESCMAALLSSSSPKCTACQESIVKDKVFKDNCCKREILALQIYCRNESRG
CAEQLMLGHLLVHLKNDCHFEELPCVRPDCKEKVLRKDLRDHVEKACKYR
EATCSHCKSQVPMIALQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLS
ECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKK
VSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVI
DSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQ
VARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIR
DYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSL
FFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKK
PTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP

Vector Type

Baculovirus

Antibiotic

Amp

Comments

N ter 6His for baculovirus expression.

Estimated MW of expressed protein : 67697 Daltons

100042 R 1000 1001 1047 19007