USP17 | Ubiquitylation Resource

Plasmid

pFBDual6P-1 USP17

Parent Plasmid

pFastbacDual 6P1

Expressed

GST USP17

DU Number

DU15659

Genbank

NP_001099132

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGAGGACGACTCACTCTACTTGGGAGGTGAGTGGCAGTTCAA
CCACTTTCCAAAACTCACATCTTCTCGGCCCGATGCAGCTTTTGCTGAAA
TCCAGCGGACTTCTCTCCCTGAGAAGTCACCACTCTCATGTGAGACCCGT
GTCGACCTCTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAG
GGAGAAGCTTCCTCTGAGTAGCAGGAGACCTGCTGCGGTGGGGGCTGGGC
TCCAGAATATGGGAAATACCTGCTACGTGAACGCTTCCTTGGAGTGGCTG
ACATACACACCGCCCCTTGCCAACTACATGCTGTCCCGGGAGCACTCTCA
AACGTGTCATCGTCACAAGGGCTGCATGCTCTGTACTATGCAAGCTCACA
TCACACGGGCCCTCCACAATCCTGGCCACGTCATCCAGCCCTCACAGGCA
TTGGCTGCTGGCTTCCATAGAGGCAAGCAGGAAGATGCTCATGAATTTCT
CATGTTCACTGTGGATGCCATGAAAAAGGCATGCCTTCCCGGGCACAAGC
AGGTAGATCATCACTCTAAGGACACCACCCTCATCCACCAAATATTTGGA
GGCTACTGGAGATCTCAAATCAAGTGTCTCCACTGCCACGGCATTTCAGA
CACTTTTGACCCTTACCTGGACATCGCCCTGGATATCCAGGCAGCTCAGA
GTGTCCAGCAAGCTTTGGAACAGTTGGTGAAGCCCGAAGAACTCAATGGA
GAGAATGCCTATCATTGTGGTGTTTGTCTCCAGAGGGCGCCGGCCTCCAA
GACGTTAACTTTACACACCTCTGCCAAGGTCCTCATCCTTGTATTGAAGA
GATTCTCCGATGTCACAGGCAACAAGATTGCCAAGAATGTGCAATATCCT
GAGTGCCTTGACATGCAGCCATACATGTCTCAGCAGAACACAGGACCTCT
CGTCTATGTCCTCTATGCTGTGCTGGTCCACGCTGGGTGGAGTTGTCACA
ACGGACATTACTTCTCTTATGTCAAAGCTCAAGAAGGCCAGTGGTATAAA
ATGGATGATGCCGAGGTCACCGCCTCTAGCATCACTTCTGTCCTGAGTCA
ACAGGCCTACGTCCTCTTTTACATCCAGAAGAGTGAATGGGAAAGACACA
GTGAGAGTGTGTCAAGAGGCAGGGAACCAAGAGCCCTTGGCGCAGAAGAC
ACAGACAGGCGAGCAACGCAAGGAGAGCTCAAGAGAGACCACCCCTGCCT
CCAGGCCCCCGAGTTGGACGAGCACTTGGTGGAAAGAGCCACTCAGGAAA
GCACCTTAGACCACTGGAAATTCCTTCAAGAGCAAAACAAAACGAAGCCT
GAGTTCAACGTCAGAAAAGTCGAAGGTACCCTGCCTCCCGACGTACTTGT
GATTCATCAATCAAAATACAAGTGTGGGATGAAGAACCATCATCCTGAAC
AGCAAAGCTCCCTGCTAAACCTCTCTTCGACGACACCGACACATCAGGAG
TCCATGAACACTGGCACACTCGCTTCCCTGCGAGGGAGGGCCAGGAGATC
CAAAGGGAAGAACAAACACAGCAAGAGGGCTCTGCTTGTGTGCCAGTGAG
CGGCCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEDDSLYLGGEWQFNHFPK
LTSSRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVARQLAPREKLP
LSSRRPAAVGAGLQNMGNTCYVNASLEWLTYTPPLANYMLSREHSQTCHR
HKGCMLCTMQAHITRALHNPGHVIQPSQALAAGFHRGKQEDAHEFLMFTV
DAMKKACLPGHKQVDHHSKDTTLIHQIFGGYWRSQIKCLHCHGISDTFDP
YLDIALDIQAAQSVQQALEQLVKPEELNGENAYHCGVCLQRAPASKTLTL
HTSAKVLILVLKRFSDVTGNKIAKNVQYPECLDMQPYMSQQNTGPLVYVL
YAVLVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVTASSITSVLSQQAYV
LFYIQKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPE
LDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQS
KYKCGMKNHHPEQQSSLLNLSSTTPTHQESMNTGTLASLRGRARRSKGKN
KHSKRALLVCQ*

Vector Type

Baculovirus

Antibiotic

Amp

Comments

N ter GST for baculovirus expression.

Estimated MW of expressed protein : 86572 Daltons

100042 R 1000 1001 1047 19007