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IPAH9.8

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Plasmid
pEBG6P-IPAH9.8
Parent Plasmid
pEBG6P
Expressed
GST IPAH9.8
DU Number
DU32827
Genbank
AY206445.1
Tag
GST
Species
Shigella flexneri
Sequence of Insert
GGATCCATGTTACCGATAAATAATAACTTTTCATTGCCCCAAAATTCTTT
TTATAACACTATTTCCGGTACATATGCTGATTACTTTTCAGCATGGGATA
AATGGGAAAAACAAGCGCTCCCCGGTGAAGAGCGTGATGAGGCTGTCTCC
CGACTTAAAGAATGTCTTATCAATAATTCCGATGAACTTCGACTGGACCG
TTTAAATCTGTCCTCGCTACCTGACAACTTACCAGCTCAGATAACGCTGC
TCAATGTATCATATAATCAATTAACTAACCTACCTGAACTGCCTGTTACG
CTAAAAAAATTATATTCCGCCAGCAATAAATTATCAGAATTGCCCGTGCT
ACCTCCTGCGCTGGAGTCACTTCAGGTACAACACAATGAGCTGGAAAACC
TGCCAGCTTTACCCGATTCGTTATTGACTATGAATATCAGCTATAACGAA
ATAGTCTCCTTACCATCGCTCCCACAGGCTCTTAAAAATCTCAGAGCGAC
CCGTAATTTCCTCACTGAGCTACCAGCATTTTCTGAGGGAAATAATCCCG
TTGTCAGAGAGTATTTTTTTGATAGAAATCAGATAAGTCATATCCCGGAA
AGCATTCTTAATCTGAGGAATGAATGTTCAATACATATTAGTGATAACCC
ATTATCATCCCATGCTCTGCAAGCCCTGCAAAGATTAACCTCTTCGCCGG
ACTACCACGGCCCACGGATTTACTTCTCCATGAGTGACGGACAACAGAAT
ACACTCCATCGCCCCCTGGCTGATGCCGTGACAGCATGGTTCCCGGAAAA
CAAACAATCTGATGTATCACAGATATGGCATGCTTTTGAACATGAAGAGC
ATGCCAACACCTTTTCCGCGTTCCTTGACCGCCTTTCCGATACCGTCTCT
GCACGCAATACCTCCGGATTCCGTGAACAGGTCGCTGCATGGCTGGAAAA
ACTCAGTGCCTCTGCGGAGCTTCGACAGCAGTCTTTCGCTGTTGCTGCTG
ATGCCACTGAGAGCTGTGAGGACCGTGTCGCGCTCACATGGAACAATCTC
CGGAAAACCCTCCTGGTCCATCAGGCATCAGAAGGCCTTTTCGATAATGA
TACCGGCGCTCTGCTCTCCCTGGGCAGGGAAATGTTCCGCCTCGAAATTC
TGGAGGACATTGCCCGGGATAAAGTCAGAACTCTCCATTTTGTGGATGAG
ATAGAAGTCTACCTGGCCTTCCAGACCATGCTCGCAGAGAAACTTCAGCT
CTCCACTGCCGTGAAGGAAATGCGTTTCTATGGCGTGTCGGGAGTGACAG
CAAATGACCTCCGCACTGCCGAAGCCATGGTCAGAAGCCGTGAAGAGAAT
GAATTTACGGACTGGTTCTCCCTCTGGGGACCATGGCATGCTGTACTGAA
GCGTACGGAAGCTGACCGCTGGGCGCTGGCAGAAGAGCAGAAATATGAGA
TGCTGGAGAATGAGTACCCTCAGAGGGTGGCTGACCGGCTGAAAGCATCA
GGTCTGAGCGGTGATGCGGATGCGGAGAGGGAAGCCGGTGCACAGGTGAT
GCGTGAGACTGAACAGCAGATTTACCGTCAGCTGACTGACGAGGTACTGG
CCCTGCGATTGCCTGAAAACGGCTCACAACTGCACCATTCATAAGCGGCC
GC
Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMLPINNNFSLPQN
SFYNTISGTYADYFSAWDKWEKQALPGEERDEAVSRLKECLINNSDELRL
DRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKLYSASNKLSELP
VLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALKNLR
ATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISD
NPLSSHALQALQRLTSSPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFP
ENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWL
EKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLFD
NDTGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKL
QLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAV
LKRTEADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQ
VMRETEQQIYRQLTDEVLALRLPENGSQLHHS*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter GST for mammalian expression

Estimated MW of expressed protein : 89409 Daltons
100042 R 1000 1001 1047 19007
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