USP1 | Ubiquitylation Resource

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Plasmid

pGEX6P-1-USP1

Parent Plasmid

pGEX6P1

Expressed

GST USP1

DU Number

DU15631

Genbank

NP_003359.3

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGCCTGGTGTCATACCTAGTGAAAGTAATGGACTTTCAAGAGG
TAGCCCTTCAAAGAAAAACAGACTTTCCTTAAAGTTTTTTCAGAAAAAGG
AAACTAAGAGAGCTTTGGATTTCACAGATTCTCAAGAAAATGAAGAAAAA
GCTTCTGAATATAGAGCATCTGAAATTGATCAAGTTGTTCCTGCAGCACA
GTCTTCACCTATAAACTGTGAGAAGAGAGAAAACTTGTTACCATTTGTGG
GACTGAATAATCTCGGCAATACTTGCTATCTTAATAGTATACTTCAGGTA
TTATATTTTTGTCCCGGTTTTAAATCTGGAGTAAAGCACTTATTTAATAT
TATTTCAAGGAAGAAAGAAGCTCTAAAGGATGAAGCCAATCAAAAAGACA
AGGGAAATTGCAAAGAAGATTCTTTGGCAAGTTATGAATTGATATGCAGT
TTACAGTCCTTAATCATTTCGGTTGAACAGCTCCAGGCTAGTTTTCTCTT
AAATCCAGAGAAATATACTGATGAACTTGCCACTCAGCCAAGGCGACTGC
TTAACACACTGAGGGAACTCAACCCTATGTATGAAGGATATCTACAGCAT
GATGCACAGGAAGTATTACAATGTATTTTGGGAAACATTCAAGAAACATG
CCAACTCCTAAAAAAAGAAGAAGTAAAAAATGTGGCAGAATTACCTACTA
AGGTAGAAGAAATACCTCATCCGAAAGAGGAAATGAATGGTATTAACAGC
ATAGAGATGGACAGTATGAGGCATTCTGAAGACTTTAAAGAGAAACTCCC
AAAAGGAAATGGGAAAAGAAAAAGTGACACTGAATTTGGTAACATGAAGA
AAAAAGTTAAATTATCCAAGGAACACCAGTCATTGGAAGAGAACCAGAGA
CAAACTAGATCAAAAAGAAAAGCTACAAGTGATACATTAGAGAGTCCTCC
TAAAATAATTCCCAAGTATATTTCTGAAAATGAGAGTCCAAGACCCTCAC
AAAAGAAATCAAGAGTTAAAATAAATTGGTTAAAGTCTGCAACTAAGCAA
CCCAGCATTCTTTCTAAATTTTGTAGTCTGGGAAAAATAACAACAAACCA
AGGAGTCAAAGGACAATCTAAAGAAAATGAATGTGATCCTGAAGAGGACT
TGGGGAAGTGTGAAAGTGATAACACAACTAATGGTTGTGGACTTGAATCT
CCAGGAAATACTGTTACACCTGTAAATGTTAATGAAGTTAAACCCATAAA
CAAAGGTGAAGAACAAATTGGTTTTGAGCTAGTGGAGAAATTATTTCAAG
GTCAGCTGGTATTAAGGACGCGTTGCTTGGAATGTGAAAGTTTAACAGAA
AGAAGAGAAGATTTTCAAGACATCAGTGTGCCAGTACAAGAAGATGAGCT
TTCCAAAGTAGAGGAGAGTTCTGAAATTTCTCCAGAGCCAAAAACAGAAA
TGAAGACCCTGAGATGGGCAATTTCACAATTTGCTTCAGTAGAAAGGATT
GTAGGAGAAGATAAATATTTCTGTGAAAACTGCCATCATTATACTGAAGC
TGAACGAAGTCTTTTGTTTGACAAAATGCCTGAAGTTATAACTATTCATT
TGAAGTGCTTTGCTGCTAGTGGTTTGGAGTTTGATTGTTATGGTGGTGGA
CTTTCCAAGATCAACACTCCTTTATTGACACCTCTTAAATTGTCACTAGA
AGAATGGAGCACAAAGCCAACTAACGACAGCTATGGATTATTTGCGGTTG
TGATGCATAGTGGCATTACAATTAGTAGTGGGCATTACACTGCTTCTGTT
AAAGTCACTGACCTTAACAGTTTAGAACTAGATAAAGGAAATTTTGTGGT
TGACCAAATGTGTGAAATAGGTAAGCCAGAACCATTGAATGAGGAGGAAG
CAAGGGGTGTGGTTGAGAATTATAATGATGAAGAAGTGTCAATTAGAGTT
GGTGGAAATACACAGCCAAGTAAAGTTTTGAACAAAAAAAATGTAGAAGC
TATTGGACTTCTTGGAGGACAAAAGAGCAAAGCAGATTATGAGCTATACA
ACAAAGCCTCTAATCCTGATAAGGTTGCTAGTACAGCGTTTGCTGAAAAT
AGAAATTCTGAGACTAGTGATACTACTGGGACCCATGAATCTGATAGAAA
CAAGGAATCCAGTGACCAAACAGGCATTAATATTAGTGGATTTGAGAACA
AAATTTCATACGTAGTGCAAAGCTTAAAGGAGTATGAGGGGAAGTGGTTG
CTTTTTGATGATTCTGAAGTCAAAGTTACTGAAGAGAAGGACTTTCTGAA
TTCTCTTTCCCCTTCTACATCTCCTACTTCTACTCCTTACTTGCTATTTT
ATAAGAAATTATAGGCGGCCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPGVIPSESNGLSRGSPSK
KNRLSLKFFQKKETKRALDFTDSQENEEKASEYRASEIDQVVPAAQSSPI
NCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKSGVKHLFNIISRK
KEALKDEANQKDKGNCKEDSLASYELICSLQSLIISVEQLQASFLLNPEK
YTDELATQPRRLLNTLRELNPMYEGYLQHDAQEVLQCILGNIQETCQLLK
KEEVKNVAELPTKVEEIPHPKEEMNGINSIEMDSMRHSEDFKEKLPKGNG
KRKSDTEFGNMKKKVKLSKEHQSLEENQRQTRSKRKATSDTLESPPKIIP
KYISENESPRPSQKKSRVKINWLKSATKQPSILSKFCSLGKITTNQGVKG
QSKENECDPEEDLGKCESDNTTNGCGLESPGNTVTPVNVNEVKPINKGEE
QIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVE
ESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSL
LFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWST
KPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMC
EIGKPEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLL
GGQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESS
DQTGINISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSP
STSPTSTPYLLFYKKL*

Vector Type

Bacterial

Antibiotic

Amp

Comments

N ter GST for bacterial expression.

Estimated MW of expressed protein : 115029 Daltons

100042 R 1000 1001 1047 19007

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