TRAF6 | Ubiquitylation Resource

Plasmid

pGEX6P-1-TRAF6 I72A

Parent Plasmid

pGEX6P1

Expressed

GST TRAF6 I72A

DU Number

DU12939

Genbank

NM_004620

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCAGTCTGCTAAACTGTGAAAACAGCTGTGGATTCAGCCAGTCTGA
AAGTGACTGCTGTGTGGCCATGGCCAGCTCCTGTAGCGCTGTAACAAAAG
ATGATAGTGTGGGTGGAACTGCCAGCACGGGGAACCTCTCCAGCTCATTT
ATGGAGGAGATCCAGGGATATGATGTAGAGTTTGACCCACCCCTGGAAAG
CAAGTATGAATGCCCCGCCTGCTTGATGGCATTACGAGAAGCAGTGCAAA
CGCCATGCGGCCATAGGTTCTGCAAAGCCTGCATCATAAAATCAATAAGG
GATGCAGGTCACAAATGTCCAGTTGACAATGAAATACTGCTGGAAAATCA
ACTATTTCCAGACAATTTTGCAAAACGTGAGATTCTTTCTCTGATGGTGA
AATGTCCAAATGAAGGTTGTTTGCACAAGATGGAACTGAGACATCTTGAG
GATCATCAGGCACATTGTGAGTTTGCTCTTATGGATTGTCCCCAATGCCA
GCGTCCCTTCCAAAAATTCCATATTAATATTCACATTCTGAAGGATTGTC
CAAGGAGACAGGTTTCTTGTGACAACTGTGCTGCATCAATGGCATTTGAA
GATAAAGAGATCCATGACCAGAACTGTCCTTTGGCAAATGTCATCTGTGA
ATACTGCAATACTATACTCATCAGAGAACAGATGCCTAATCATTATGATC
TAGACTGCCCTACAGCCCCAATTCCATGCACATTCAGTACTTTTGGTTGC
CATGAAAAGATGCAGAGGAATCACTTGGCACGCCACCTACAAGAGAACAC
CCAGTCACACATGAGAATGTTGGCCCAGGCTGTTCATAGTTTGAGCGTTA
TACCCGACTCTGGGTATATCTCAGAGGTCCGGAATTTCCAGGAAACTATT
CACCAGTTAGAGGGTCGCCTTGTAAGACAAGACCATCAAATCCGGGAGCT
GACTGCTAAAATGGAAACTCAGAGTATGTATGTAAGTGAGCTCAAACGAA
CCATTCGAACCCTTGAGGACAAAGTTGCTGAAATCGAAGCACAGCAGTGC
AATGGAATTTATATTTGGAAGATTGGCAACTTTGGAATGCATTTGAAATG
TCAAGAAGAGGAGAAACCTGTTGTGATTCATAGCCCTGGATTCTACACTG
GCAAACCCGGGTACAAACTGTGCATGCGCTTGCACCTTCAGTTACCGACT
GCTCAGCGCTGTGCAAACTATATATCCCTTTTTGTCCACACAATGCAAGG
AGAATATGACAGCCACCTCCCTTGGCCCTTCCAGGGTACAATACGCCTTA
CAATTCTTGATCAGTCTGAAGCACCTGTAAGGCAAAACCACGAAGAGATA
ATGGATGCCAAACCAGAGCTGCTTGCTTTCCAGCGACCCACAATCCCACG
GAACCCAAAAGGTTTTGGCTATGTAACTTTTATGCATCTGGAAGCCCTAA
GACAAAGAACTTTCATTAAGGATGACACATTATTAGTGCGCTGTGAGGTC
TCCACCCGCTTTGACATGGGTAGCCTTCGGAGGGAGGGTTTTCAGCCACG
AAGTACTGATGCAGGGGTATAGGCGGCCGCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGSSLLNCENSCGFSQSESD
CCVAMASSCSAVTKDDSVGGTASTGNLSSSFMEEIQGYDVEFDPPLESKY
ECPACLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLF
PDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCPQCQRP
FQKFHINIHILKDCPRRQVSCDNCAASMAFEDKEIHDQNCPLANVICEYC
NTILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQS
HMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDHQIRELTA
KMETQSMYVSELKRTIRTLEDKVAEIEAQQCNGIYIWKIGNFGMHLKCQE
EEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEY
DSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPELLAFQRPTIPRNP
KGFGYVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRREGFQPRST
DAGV*

Vector Type

Bacterial

Antibiotic

Amp

Comments

N TERMINAL GST FUSION FOR BACTERIAL EXPRESSION.

Estimated MW of expressed protein : 86426 Daltons

100042 R 1000 1001 1047 19007