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Plasmid
pGEX6P-1-FLAG Ubiquitin K29wt
Parent Plasmid
pGEX6P1
Expressed
GST-FLAG Ubiquitin K29wt
DU Number
DU8980
Genbank
P62988
Tag
GST
FLAG
Species
Human
Sequence of Insert
View Sequence
GAATTCGGATCTGCCACCATGGACTACAAGGACGACGATGACAAGCAAAT
CTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGAAGTGGAGCCGA
GTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACAGGGAAGGCATC
CCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGG
ACGCACCCTGTCTGACTACCACATCCAGAGAGAGTCCACCCTGCACCTGG
TCCTCCGTCTCTTAGGTGGGTGATAACTCGAG
CTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGAAGTGGAGCCGA
GTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACAGGGAAGGCATC
CCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGG
ACGCACCCTGTCTGACTACCACATCCAGAGAGAGTCCACCCTGCACCTGG
TCCTCCGTCTCTTAGGTGGGTGATAACTCGAG
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFGSATMDYKDDDDKQIF
VRTLTGRTITLEVEPSDTIENVRAKIQDREGIPPDQQRLIFAGRQLEDGR
TLSDYHIQRESTLHLVLRLLGG*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFGSATMDYKDDDDKQIF
VRTLTGRTITLEVEPSDTIENVRAKIQDREGIPPDQQRLIFAGRQLEDGR
TLSDYHIQRESTLHLVLRLLGG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N terminal GST/FLAG for bacterial expression.
100042 R 1000 1001 1047 19007
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