USP7 | Ubiquitylation Resource

Plasmid

pEBG6P-USP7 150-600

Parent Plasmid

pEBG6P

Expressed

GST USP7 150-600

DU Number

DU32706

Genbank

AK302912.1

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCTTCAGTCGTCGTATTAGTCATTTGTTCTTCCATAAAGAAAATGA
TTGGGGATTTTCCAATTTTATGGCCTGGAGTGAAGTGACCGATCCTGAGA
AAGGATTTATAGATGATGACAAAGTTACCTTTGAAGTCTTTGTACAGGCG
GATGCTCCCCATGGAGTTGCGTGGGATTCAAAGAAGCACACAGGCTACGT
CGGCTTAAAGAATCAGGGAGCGACTTGTTACATGAACAGCCTGCTACAGA
CGTTATTTTTCACGAATCAGCTACGAAAGGCTGTGTACATGATGCCAACC
GAGGGGGATGATTCGTCTAAAAGCGTCCCTTTAGCATTACAAAGAGTGTT
CTATGAATTACAGCATAGTGATAAACCTGTAGGAACAAAAAAGTTAACAA
AGTCATTTGGGTGGGAAACTTTAGATAGCTTCATGCAACATGATGTTCAG
GAGCTTTGTCGAGTGTTGCTCGATAATGTGGAAAATAAGATGAAAGGCAC
CTGTGTAGAGGGCACCATACCCAAATTATTCCGCGGCAAAATGGTGTCCT
ATATCCAGTGTAAAGAAGTAGACTATCGGTCTGATAGAAGAGAAGATTAT
TATGATATCCAGCTAAGTATCAAAGGAAAGAAAAATATATTTGAATCATT
TGTGGATTATGTGGCAGTAGAACAGCTCGATGGGGACAATAAATACGACG
CTGGGGAACATGGCTTACAGGAAGCAGAGAAAGGTGTGAAATTCCTAACA
TTGCCACCAGTGTTACATCTACAACTGATGAGATTTATGTATGACCCTCA
GACGGACCAAAATATCAAGATCAATGATAGGTTTGAATTCCCAGAGCAGT
TACCACTTGATGAATTTTTGCAAAAAACAGATCCTAAGGACCCTGCAAAT
TATATTCTTCATGCAGTCCTGGTTCATAGTGGAGATAATCATGGTGGACA
TTATGTGGTTTATCTAAACCCCAAAGGGGATGGCAAATGGTGTAAATTTG
ATGACGACGTGGTGTCAAGGTGTACTAAAGAGGAAGCAATTGAGCACAAT
TATGGGGGTCACGATGACGACCTGTCTGTTCGACACTGCACTAATGCTTA
CATGTTAGTCTACATCAGGGAATCAAAACTGAGTGAAGTTTTACAGGCGG
TCACCGACCATGATATTCCTCAGCAGTTGGTGGAGCGATTACAAGAAGAG
AAAAGGATCGAGGCTCAGAAGCGGAAGGAGCGGCAGGAAGCCCATCTCTA
TATGCAAGTGCAGATAGTCGCAGAGGACCAGTTTTGTGGCCACCAAGGGA
ATGACATGTACGATGAAGAAAAAGTGAAATACACTGTGTTCAAAGTATTG
AAGAACTCCTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSFSRRISHLFFHKE
NDWGFSNFMAWSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTG
YVGLKNQGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQR
VFYELQHSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMK
GTCVEGTIPKLFRGKMVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFE
SFVDYVAVEQLDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYD
PQTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHG
GHYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTN
AYMLVYIRESKLSEVLQAVTDHDIPQQLVERLQEEKRIEAQKRKERQEAH
LYMQVQIVAEDQFCGHQGNDMYDEEKVKYTVFKVLKNS*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter GST for mammalian expression

Estimated MW of expressed protein : 79826 Daltons

100042 R 1000 1001 1047 19007