TRIM28 | Ubiquitylation Resource

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Plasmid

pEBG6P-TRIM 28

Parent Plasmid

pEBG6P

Expressed

GST-TRIM 28

DU Number

DU12454

Genbank

NP_005753.1

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GCGGCCGCGATGGCGGCCTCCGCGGCGGCAGCCTCGGCAGCAGCGGCCTC
GGCCGCCTCTGGCAGCCCGGGCCCGGGCGAGGGCTCCGCTGGCGGCGAAA
AGCGCTCCACCGCCCCTTCGGCCGCAGCCTCGGCCTCTGCCTCAGCCGCG
GCGTCGTCGCCCGCGGGGGGCGGCGCCGAGGCGCTGGAGCTGCTGGAGCA
CTGCGGCGTGTGCAGAGAGCGCCTGCGACCCGAGAGGGAGCCCCGCCTGC
TGCCCTGTTTGCACTCGGCCTGTAGTGCCTGCTTAGGGCCCGCGGCCCCC
GCCGCCGCCAACAGCTCGGGGGACGGCGGGGCGGCGGGCGACGGCACCGT
GGTGGACTGTCCCGTGTGCAAGCAACAGTGCTTCTCCAAAGACATCGTGG
AGAATTATTTCATGCGTGATAGTGGCAGCAAGGCTGCCACCGACGCCCAG
GATGCGAACCAGTGCTGCACTAGCTGTGAGGATAATGCCCCAGCCACCAG
CTACTGTGTGGAGTGCTCGGAGCCTCTGTGTGAGACCTGTGTAGAGGCGC
ACCAGCGGGTGAAGTACACCAAGGACCATACTGTGCGCTCTACTGGGCCA
GCCAAGTCTCGGGATGGTGAACGTACTGTCTATTGCAACGTACACAAGCA
TGAACCCCTTGTGCTGTTTTGTGAGAGCTGTGATACTCTCACCTGCCGAG
ACTGCCAGCTCAATGCCCACAAGGACCACCAGTACCAGTTCTTAGAGGAT
GCAGTGAGGAACCAGCGCAAGCTCCTGGCCTCACTGGTGAAGCGCCTTGG
GGACAAACATGCAACATTGCAGAAGAGCACCAAGGAGGTTCGCAGCTCAA
TCCGCCAGGTGTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATG
GCCATCCTGCAGATCATGAAGGAGCTGAATAAGCGGGGCCGTGTGCTGGT
CAATGATGCCCAGAAGGTGACTGAGGGGCAGCAGGAGCGCCTGGAGCGGC
AGCACTGGACCATGACCAAGATCCAGAAGCACCAGGAGCACATTCTGCGC
TTTGCCTCTTGGGCTCTGGAGAGTGACAACAACACAGCCCTTTTGCTTTC
TAAGAAGTTGATCTACTTCCAGCTGCACCGGGCCCTCAAGATGATTGTGG
ATCCCGTGGAGCCACATGGCGAGATGAAGTTTCAGTGGGACCTCAATGCC
TGGACCAAGAGTGCCGAGGCCTTTGGCAAGATTGTGGCAGAGCGTCCTGG
CACTAACTCAACAGGCCCTGCACCCATGGCCCCTCCAAGAGCCCCAGGGC
CCCTGAGCAAGCAGGGCTCTGGCAGCAGCCAGCCCATGGAGGTGCAGGAA
GGCTATGGCTTTGGGTCAGGAGATGATCCCTACTCAAGTGCAGAGCCCCA
TGTGTCAGGTGTGAAACGGTCCCGCTCAGGTGAGGGCGAGGTGAGCGGCC
TTATGCGCAAGGTGCCACGAGTGAGCCTTGAACGCCTGGACCTGGACCTC
ACAGCTGACAGCCAGCCACCCGTCTTCAAGGTCTTCCCAGGCAGTACCAC
TGAGGACTACAACCTTATTGTTATTGAACGTGGCGCTGCCGCTGCAGCTA
CCGGCCAGCCAGGGACTGCGCCTGCAGGAACCCCTGGTGCCCCACCCCTG
GCTGGCATGGCCATTGTCAAGGAGGAGGAGACGGAGGCTGCCATTGGAGC
CCCTCCTACTGCCACTGAGGGCCCTGAGACCAAACCTGTGCTTATGGCTC
TTGCGGAGGGTCCTGGTGCTGAGGGTCCCCGCCTGGCCTCACCTAGTGGC
AGCACCAGCTCAGGGCTGGAGGTGGTGGCTCCTGAGGGTACCTCAGCCCC
AGGTGGTGGCCCGGGAACCCTGGATGACAGTGCCACCATTTGCCGTGTCT
GCCAGAAGCCAGGCGATCTGGTTATGTGCAACCAGTGTGAGTTTTGTTTC
CACCTGGACTGTCACCTGCCGGCCCTGCAGGATGTACCAGGGGAGGAGTG
GAGCTGCTCACTCTGCCATGTGCTCCCTGACCTGAAGGAGGAGGATGGCA
GCCTCAGCCTGGATGGTGCAGACAGCACTGGCGTGGTGGCCAAGCTCTCA
CCAGCCAACCAGCGGAAATGTGAGCGTGTACTGCTGGCCCTATTCTGTCA
CGAACCCTGCCGCCCCCTGCATCAGCTGGCTACCGACTCCACCTTCTCCC
TGGACCAGCCCGGTGGCACCCTGGATCTGACCCTGATCCGTGCCCGCCTC
CAGGAGAAGTTGTCACCTCCCTACAGCTCCCCACAGGAGTTTGCCCAGGA
TGTGGGCCGCATGTTCAAGCAATTCAACAAGTTAACTGAGGACAAGGCAG
ACGTGCAGTCCATCATCGGCCTGCAGCGCTTCTTCGAGACGCGCATGAAC
GAGGCCTTCGGTGACACCAAGTTCTCTGCTGTGCTGGTGGAGCCCCCGCC
GATGAGCCTGCCTGGTGCTGGCCTGAGTTCCCAGGAGCTGTCTGGTGGCC
CTGGTGATGGCCCCTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMAAS
AAAASAAAASAASGSPGPGEGSAGGEKRSTAPSAAASASASAAASSPAGG
GAEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPAAPAAANSSG
DGGAAGDGTVVDCPVCKQQCFSKDIVENYFMRDSGSKAATDAQDANQCCT
SCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKSRDGE
RTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRK
LLASLVKRLGDKHATLQKSTKEVRSSIRQVSDVQKRVQVDVKMAILQIMK
ELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALE
SDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEA
FGKIVAERPGTNSTGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSG
DDPYSSAEPHVSGVKRSRSGEGEVSGLMRKVPRVSLERLDLDLTADSQPP
VFKVFPGSTTEDYNLIVIERGAAAAATGQPGTAPAGTPGAPPLAGMAIVK
EEETEAAIGAPPTATEGPETKPVLMALAEGPGAEGPRLASPSGSTSSGLE
VVAPEGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLP
ALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKC
ERVLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEKLSPP
YSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTK
FSAVLVEPPPMSLPGAGLSSQELSGGPGDGP

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter GST for mammalian expression.

Estimated MW of expressed protein : 116749 Daltons

100042 R 1000 1001 1047 19007

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