USP37

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Plasmid
pcDNA5-FRT/TO-FLAG USP37 M73-end
Parent Plasmid
pcDNA5 FRT/TO FLAG2
Expressed
FLAG USP37 M73-end
DU Number
DU15141
Genbank
NM_020935.2
Tag
FLAG
Species
Human
Sequence of Insert
GCGGCCGCGATGTTAACTCTGCAAGATAACAGCTTCTTGTCTATTGACAA
AGTACCAAGTAAGGATGCAGAGGAAATGAGGTTGTTTCTAGATGCAGTCC
ATCAAAACAGACTTCCTGCAGCCATGAAACCGTCTCAGGGGTCTGGTAGT
TTTGGAGCCATTCTGGGCAGCAGGACCTCACAGAAGGAAACCAGCAGGCA
GCTTTCTTACTCAGACAATCAGGCTTCTGCAAAAAGAGGAAGTTTGGAAA
CTAAAGATGATATTCCATTTCGAAAAGTTCTTGGTAATCCGGGTAGAGGA
TCGATTAAGACTGTAGCAGGAAGTGGAATAGCTCGGACGATTCCTTCTTT
GACATCTACTTCAACACCTCTTAGATCAGGGTTGCTAGAAAATCGTACTG
AAAAGAGGAAAAGAATGATATCAACTGGCTCAGAATTGAATGAAGATTAC
CCTAAGGAAAATGATTCATCATCGAACAACAAGGCCATGACAGATCCCTC
CAGAAAGTATTTAACCAGCAGTAGAGAAAAGCAGCTGAGTTTGAAACAGT
CAGAAGAGAATAGGACATCAGGGCTTTTACCTTTACAGTCATCATCCTTT
TATGGTAGCAGAGCTGGATCCAAGGAACACTCTTCTGGTGGCACTAACTT
AGACAGGACTAATGTTTCAAGCCAGACTCCCTCTGCCAAAAGAAGTTTGG
GATTTCTTCCTCAGCCAGTTCCTCTTTCTGTTAAAAAACTGAGGTGTAAC
CAGGATTACACTGGCTGGAATAAACCAAGAGTGCCCCTTTCCTCTCACCA
ACAGCAGCAACTGCAGGGCTTCTCCAATTTGGGAAATACCTGCTATATGA
ATGCTATTCTACAATCTCTATTTTCACTCCAGTCATTTGCAAATGACTTG
CTTAAACAAGGTATCCCATGGAAGAAAATTCCACTCAATGCACTTATCAG
ACGCTTTGCACACTTGCTTGTTAAAAAAGATATCTGTAATTCAGAGACCA
AAAAGGATTTACTCAAGAAGGTTAAAAATGCCATTTCAGCTACAGCAGAG
AGATTCTCTGGTTATATGCAGAATGATGCTCATGAATTTTTAAGTCAGTG
TTTGGACCAGCTGAAAGAAGATATGGAAAAATTAAATAAAACTTGGAAGA
CTGAACCTGTTTCTGGAGAAGAAAATTCACCAGATATTTCAGCTACCAGA
GCATACACTTGCCCTGTTATTACTAATTTGGAGTTTGAGGTTCAGCACTC
CATCATTTGTAAAGCATGTGGAGAGATTATCCCCAAAAGAGAACAGTTTA
ATGACCTCTCTATTGACCTTCCTCGTAGGAAAAAACCACTCCCTCCTCGT
TCAATTCAAGATTCTCTTGATCTTTTCTTTAGGGCCGAAGAACTGGAGTA
TTCTTGTGAGAAGTGTGGTGGGAAGTGTGCTCTTGTCAGGCACAAATTTA
ACAGGCTTCCTAGGGTCCTCATTCTCCATTTGAAACGATATAGCTTCAAT
GTGGCTCTCTCGCTTAACAATAAGATTGGGCAGCAAGTCATCATTCCAAG
ATACCTGACCCTGTCATCTCATTGCACTGAAAATACAAAACCACCTTTTA
CCCTTGGTTGGAGTGCACATATGGCAATTTCTAGACCATTGAAAGCCTCT
CAAATGGTGAATTCCTGCATCACCAGCCCTTCTACACCTTCAAAGAAATT
CACCTTCAAATCCAAGAGCTCCTTGGCTTTATGCCTTGATTCAGACAGTG
AGGATGAGCTAAAACGTTCTGTGGCCCTCAGCCAGAGACTTTGTGAAATG
TTAGGCAACGAACAGCAGCAGGAAGACCTGGAAAAAGATTCAAAATTATG
CCCAATAGAGCCTGACAAGTCTGAATTGGAAAACTCAGGATTTGACAGAA
TGAGCGAAGAAGAGCTTCTAGCAGCTGTCTTGGAGATAAGTAAGAGAGAT
GCTTCACCATCTCTGAGTCATGAAGATGATGATAAGCCAACTAGCAGCCC
AGATACCGGATTTGCAGAAGATGATATTCAAGAAATGCCAGAAAATCCAG
ACACTATGGAAACTGAGAAGCCCAAAACAATCACAGAGCTGGATCCTGCC
AGTTTTACTGAGATAACTAAAGACTGTGATGAGAATAAAGAAAACAAAAC
TCCAGAAGGATCTCAGGGAGAAGTTGATTGGCTCCAGCAGTATGATATGG
AGCGTGAAAGGGAAGAGCAAGAGCTTCAGCAGGCACTGGCTCAGAGCCTT
CAAGAGCAAGAGGCTTGGGAACAGAAAGAAGATGATGACCTCAAAAGAGC
TACCGAGTTAAGTCTTCAAGAGTTTAACAACTCCTTTGTGGATGCATTGG
GTTCTGATGAGGACTCTGGAAATGAGGATGTTTTTGATATGGAGTACACA
GAAGCTGAAGCTGAGGAACTGAAAAGAAATGCTGAGACAGGAAATCTGCC
TCATTCGTACCGGCTCATCAGTGTTGTCAGTCACATTGGTAGCACTTCTT
CTTCAGGTCATTACATTAGTGATGTATATGACATTAAGAAGCAAGCGTGG
TTTACTTACAATGACCTGGAGGTATCAAAAATCCAAGAGGCTGCCGTGCA
GAGTGATCGAGATCGGAGTGGCTACATCTTCTTTTATATGCACAAGGAGA
TCTTTGATGAGCTGCTGGAAACAGAAAAGAACTCTCAGTCACTTAGCACG
GAAGTGGGGAAGACTACCCGTCAGGCCTCGTGAGCGGCCGC
Amino Acid Sequence
MDYKDDDDKGIPGIPGSTRAAAMLTLQDNSFLSIDKVPSKDAEEMRLFLD
AVHQNRLPAAMKPSQGSGSFGAILGSRTSQKETSRQLSYSDNQASAKRGS
LETKDDIPFRKVLGNPGRGSIKTVAGSGIARTIPSLTSTSTPLRSGLLEN
RTEKRKRMISTGSELNEDYPKENDSSSNNKAMTDPSRKYLTSSREKQLSL
KQSEENRTSGLLPLQSSSFYGSRAGSKEHSSGGTNLDRTNVSSQTPSAKR
SLGFLPQPVPLSVKKLRCNQDYTGWNKPRVPLSSHQQQQLQGFSNLGNTC
YMNAILQSLFSLQSFANDLLKQGIPWKKIPLNALIRRFAHLLVKKDICNS
ETKKDLLKKVKNAISATAERFSGYMQNDAHEFLSQCLDQLKEDMEKLNKT
WKTEPVSGEENSPDISATRAYTCPVITNLEFEVQHSIICKACGEIIPKRE
QFNDLSIDLPRRKKPLPPRSIQDSLDLFFRAEELEYSCEKCGGKCALVRH
KFNRLPRVLILHLKRYSFNVALSLNNKIGQQVIIPRYLTLSSHCTENTKP
PFTLGWSAHMAISRPLKASQMVNSCITSPSTPSKKFTFKSKSSLALCLDS
DSEDELKRSVALSQRLCEMLGNEQQQEDLEKDSKLCPIEPDKSELENSGF
DRMSEEELLAAVLEISKRDASPSLSHEDDDKPTSSPDTGFAEDDIQEMPE
NPDTMETEKPKTITELDPASFTEITKDCDENKENKTPEGSQGEVDWLQQY
DMEREREEQELQQALAQSLQEQEAWEQKEDDDLKRATELSLQEFNNSFVD
ALGSDEDSGNEDVFDMEYTEAEAEELKRNAETGNLPHSYRLISVVSHIGS
TSSSGHYISDVYDIKKQAWFTYNDLEVSKIQEAAVQSDRDRSGYIFFYMH
KEIFDELLETEKNSQSLSTEVGKTTRQAS*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter FLAG for mammalian expression.

Estimated MW of expressed protein : 104282 Daltons
100042 R 1000 1001 1047 19007
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