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SENP1

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Plasmid
pFBDual6P-1 SENP1
Parent Plasmid
pFastbacDual 6P1
Expressed
GST SENP1
DU Number
DU32820
Genbank
AK292060.1
Tag
GST
Species
Human
Sequence of Insert
GAATTCATGGATGATATTGCTGATAGGATGAGGATGGATGCTGGAGAAGT
GACTTTAGTGAACCACAACTCCGTATTCAAAACCCACCTCCTGCCACAAA
CAGGTTTTCCAGAGGACCAGCTTTCGCTTTCTGACCAGCAGATTTTATCT
TCCAGGCAAGGACATTTGGACCGATCTTTTACATGTTCCACAAGAAGTGC
AGCTTATAATCCAAGCTATTACTCAGATAATCCTTCCTCAGACAGTTTTC
TTGGCTCAGGCGATTTAAGAACCTTTGGCCAGAGTGCAAATGGCCAATGG
AGAAATTCTACCCCATCGTCAAGCTCATCTTTACAAAAATCAAGAAACAG
CCGAAGTCTTTACCTCGAAACCCGAAAGACCTCAAGTGGATTATCAAACA
GTTTTGCGGGAAAGTCAAACCATCACTGCCATGTATCTGCATATGAAAAA
TCTTTTCCTATTAAACCTGTTCCAAGTCCATCTTGGAGTGGTTCATGTCG
TCGAAGTCTCTTGAGCCCCAAGAAAACTCAGAGGCGACATGTTAGTACAG
CAGAAGAGACAGTTCAAGAAGAAGAAAGAGAGATTTACAGACAGCTGCTA
CAGATGGTCACAGGGAAACAGTTTACTATAGCCAAACCCACCACACATTT
TCCTTTACACCTGTCTCGATGTCTTAGTTCCAGTAAAAATACTTTGAAAG
ACTCACTGTTTAAAAATGGAAACTCTTGTGCATCTCAGATCATTGGCTCT
GATACTTCATCATCTGGATCTGCCAGCATTTTAACTAACCAGGAACAGCT
GTCCCACAGTGTATATTCCCTATCTTCTTATACCCCAGATGTTGCATTTG
GATCCAAAGATTCTGGTACTCTTCATCATCCCCATCATCACCACTCTGTT
CCACATCAGCCAGATAACTTAGCAGCTTCAAATACACAATCTGAAGGATC
AGACTCTGTGATTTTACTGAAAGTGAAAGATTCCCAGACTCCAACTCCCA
GTTCTACTTTCTTCCAGGCAGAGCTGTGGATCAAAGAATTAACTAGTGTT
TATGATTCTCGAGCACGAGAAAGATTGCGCCAGATTGACGAACAGAAGGC
ATTGGCCTTACAGCTTCAAAACCAGAGATTGCAGGAGCGGGAACATTCAG
TACATGATTCAGTAGAACTACATCTTCGTGTACCTCTTGAAAAGGAGATT
CCTGTTACTGTTGTCCAAGAAACACAAAAAAAAGGTCATAAATTAACTGA
TAGTGAAGATGAATTTCCTGAAATTACAGAGGAAATGGAGAAAGAAATAA
AGAATGTATTTCGTAATGGGAATCAGGATGAAGTTCTCAGTGAAGCATTT
CGCCTGACCATTACACGCAAAGATATTCAAACTCTAAACCATCTGAATTG
GCTCAATGATGAGATCATCAATTTCTACATGAATATGCTGATGGAGCGAA
GTAAAGAGAAGGGCTTGCCAAGTGTGCATGCATTTAATACCTTTTTCTTC
ACTAAATTAAAAACGGCTGGTTATCAGGCAGTGAAACGTTGGACAAAGAA
AGTAGATGTATTTTCTGTTGACATTCTTTTGGTGCCCATTCACCTGGGAG
TACACTGGTGTCTAGCTGTTGTGGACTTTAGAAAGAAGAATATTACCTAT
TACGACTCCATGGGTGGGATAAACAATGAAGCCTGCAGAATACTCTTGCA
ATACCTAAAGCAAGAAAGCATTGACAAGAAAAGGAAAGAGTTTGACACCA
ATGGCTGGCAGCTTTTCAGCAAGAAAAGCCAGGAGATTCCTCAGCAGATG
AATGGAAGTGACTGTGGGATGTTTGCCTGCAAATATGCTGACTGTATTAC
CAAAGACAGACCAATCAACTTCACACAGCAACACATGCCATACTTCCGGA
AGCGGATGGTCTGGGAGATCCTCCACCGAAAACTCTTGTGAGTCGAC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMDDIADRMRMDAGEVT
LVNHNSVFKTHLLPQTGFPEDQLSLSDQQILSSRQGHLDRSFTCSTRSAA
YNPSYYSDNPSSDSFLGSGDLRTFGQSANGQWRNSTPSSSSSLQKSRNSR
SLYLETRKTSSGLSNSFAGKSNHHCHVSAYEKSFPIKPVPSPSWSGSCRR
SLLSPKKTQRRHVSTAEETVQEEEREIYRQLLQMVTGKQFTIAKPTTHFP
LHLSRCLSSSKNTLKDSLFKNGNSCASQIIGSDTSSSGSASILTNQEQLS
HSVYSLSSYTPDVAFGSKDSGTLHHPHHHHSVPHQPDNLAASNTQSEGSD
SVILLKVKDSQTPTPSSTFFQAELWIKELTSVYDSRARERLRQIDEQKAL
ALQLQNQRLQEREHSVHDSVELHLRVPLEKEIPVTVVQETQKKGHKLTDS
EDEFPEITEEMEKEIKNVFRNGNQDEVLSEAFRLTITRKDIQTLNHLNWL
NDEIINFYMNMLMERSKEKGLPSVHAFNTFFFTKLKTAGYQAVKRWTKKV
DVFSVDILLVPIHLGVHWCLAVVDFRKKNITYYDSMGGINNEACRILLQY
LKQESIDKKRKEFDTNGWQLFSKKSQEIPQQMNGSDCGMFACKYADCITK
DRPINFTQQHMPYFRKRMVWEILHRKLL*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
N ter GST for insect expression. Sequence agrees with genbank except for E361D change which occurs in the original Hay construct.

Estimated MW of expressed protein : 100662 Daltons
100042 R 1000 1001 1047 19007
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