KLHL13

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Plasmid
pACT2.6 KLHL13
Parent Plasmid
pACT2.6
Expressed
GAL4-DNA-AD-HA-KLHL13
DU Number
DU33293
Genbank
NM_033495.2
Tag
GAL4 DNA-AD
HA
Species
Human
Sequence of Insert
AGATCTATGCCATTGAAATGGAAAACGAGCTCTCCTGCTATCTGGAAATTCCCAGTTCCTGTGCTTAAAACATCCAGGTCAACTCCACTTTCTCCAGCATACATATCTCTCGTGGAAGAGGAAGACCAACACATGAAATTGTCCCTTGGAGGCAGCGAAATGGGCCTCTCATCCCATTTGCAGTCTTCCAAGGCAGGACCTACACGCATCTTTACCAGCAATACCCACAGTTCTGTGGTGTTACAGGGCTTTGACCAGCTTCGACTTGAAGGATTGCTTTGTGATGTGACCCTGATGCCAGGTGACACAGATGATGCTTTCCCTGTGCATAGAGTCATGATGGCATCTGCTAGTGATTACTTCAAGGCTATGTTCACAGGTGGAATGAAAGAACAAGATTTAATGTGCATTAAACTTCATGGTGTGAGCAAAGTCGGTCTAAGGAAAATTATTGATTTCATTTATACTGCAAAGCTTTCTCTTAATATGGACAACCTTCAAGACACGCTGGAAGCTGCCAGTTTCCTACAGATTCTGCCAGTTTTGGACTTCTGTAAAGTGTTTCTCATATCTGGGGTCACTTTAGACAACTGTGTTGAAGTTGGACGGATTGCCAACACCTACAATCTAACCGAAGTGGATAAATACGTTAACAGTTTCGTCTTGAAGAATTTTCCTGCATTGCTGAGCACAGGGGAGTTCTTGAAACTCCCTTTTGAGCGTCTTGCCTTCGTGCTTTCCAGTAATAGCCTTAAGCACTGTACTGAACTTGAGCTCTTTAAGGCTACCTGTCGTTGGCTTCGCCTGGAAGAGCCTCGGATGGACTTTGCTGCAAAATTAATGAAGAACATACGATTTCCACTGATGACACCACAGGAGCTCATTAATTACGTGCAAACGGTGGATTTCATGAGAACTGACAATACTTGTGTGAATTTGCTTTTGGAAGCCAGCAATTACCAAATGATGCCATATATGCAGCCAGTTATGCAGTCAGACAGGACTGCCATTAGGTCTGACACCACTCACTTGGTTACACTAGGAGGAGTGCTGAGGCAGCAGCTGGTTGTCAGTAAGGAATTGCGCATGTATGATGAAAAGGCCCATGAGTGGAAATCGTTAGCCCCCATGGATGCCCCAAGGTACCAGCATGGCATCGCCGTCATTGGAAATTTTCTCTATGTGGTTGGCGGACAGAGTAATTATGATACAAAAGGAAAAACGGCAGTTGATACAGTCTTCAGATTTGATCCTCGATACAATAAATGGATGCAAGTTGCATCTTTAAATGAAAAGCGCACCTTCTTCCACCTAAGTGCCCTCAAAGGATATCTGTATGCAGTTGGTGGGCGAAATGCAGCAGGTGAACTGCCCACAGTAGAATGTTACAATCCAAGAACAAATGAATGGACCTATGTTGCCAAAATGAGTGAGCCCCACTATGGCCATGCTGGAACTGTGTATGGAGGAGTGATGTATATTTCAGGAGGAATTACTCATGATACTTTCCAAAAGGAGCTCATGTGCTTTGACCCTGATACTGACAAATGGATCCAGAAGGCGCCAATGACCACTGTCAGAGGTCTGCATTGCATGTGTACAGTGGGAGAAAGGCTCTATGTCATTGGTGGCAATCACTTCAGAGGAACAAGTGATTATGATGATGTCCTAAGCTGTGAATACTATTCACCTATCCTTGACCAGTGGACCCCAATTGCTGCCATGTTAAGAGGGCAGAGTGATGTTGGGGTCGCTGTCTTCGAAAATAAAATCTATGTGGTTGGGGGGTATTCTTGGAATAATCGTTGTATGGTAGAGATAGTGCAGAAATATGATCCAGATAAAGATGAATGGCATAAGGTTTTTGATCTGCCAGAATCCCTTGGTGGCATTCGTGCTTGCACACTCACAGTTTTTCCACCAGAAGAAACCACACCATCACCTTCTAGAGAGTCCCCTCTTTCTGCACCTTAAGAATTC
Amino Acid Sequence
MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYASLGGHMAMGGSMPLKWKTSSPAIWKFPVPVLKTSRSTPLSPAYISLVEEEDQHMKLSLGGSEMGLSSHLQSSKAGPTRIFTSNTHSSVVLQGFDQLRLEGLLCDVTLMPGDTDDAFPVHRVMMASASDYFKAMFTGGMKEQDLMCIKLHGVSKVGLRKIIDFIYTAKLSLNMDNLQDTLEAASFLQILPVLDFCKVFLISGVTLDNCVEVGRIANTYNLTEVDKYVNSFVLKNFPALLSTGEFLKLPFERLAFVLSSNSLKHCTELELFKATCRWLRLEEPRMDFAAKLMKNIRFPLMTPQELINYVQTVDFMRTDNTCVNLLLEASNYQMMPYMQPVMQSDRTAIRSDTTHLVTLGGVLRQQLVVSKELRMYDEKAHEWKSLAPMDAPRYQHGIAVIGNFLYVVGGQSNYDTKGKTAVDTVFRFDPRYNKWMQVASLNEKRTFFHLSALKGYLYAVGGRNAAGELPTVECYNPRTNEWTYVAKMSEPHYGHAGTVYGGVMYISGGITHDTFQKELMCFDPDTDKWIQKAPMTTVRGLHCMCTVGERLYVIGGNHFRGTSDYDDVLSCEYYSPILDQWTPIAAMLRGQSDVGVAVFENKIYVVGGYSWNNRCMVEIVQKYDPDKDEWHKVFDLPESLGGIRACTLTVFPPEETTPSPSRESPLSAP*
Vector Type
Other
Antibiotic
Amp
Comments
Expression of fragment with an N-ter GAL4 Activating Domain and an N-ter HA tag for dual-hybrid screening.

Estimated MW of expressed protein : 91196.36 Daltons
100042 R 1000 1001 1047 19007
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