HOIL1 | Ubiquitylation Resource

Plasmid

pCMV-HA-1-HOIL1 (M1-G478)

Parent Plasmid

pCMV-HA-1

Expressed

HA-HOIL1 (M1-G478)

DU Number

DU65413

Genbank

NM_031229

Tag

HA

Species

Human

Sequence of Insert

View Sequence

gaattcATGGACGAGAAGACCAAGAAAGCAGAGGAAATGGCCCTGAGCCT
CACCCGAGCAGTGGCGGGCGGGGATGAACAGGTGGCAATGAAGTGTGCCA
TCTGGCTGGCAGAGCAACGGGTGCCCCTGAGTGTGCAACTGAAGCCTGAG
GTCTCCCCAACGCAGGACATCAGGCTGTGGGTGAGCGTGGAGGATGCTCA
GATGCACACCGTCACCATCTGGCTCACAGTGCGCCCTGATATGACAGTGG
CGTCTCTCAAGGACATGGTTTTTCTGGACTATGGCTTCCCACCAGTCTTG
CAGCAGTGGGTGATTGGGCAGCGGCTGGCACGAGACCAGGAGACCCTGCA
CTCCCATGGGGTGCGGCAGAATGGGGACAGTGCCTACCTCTATCTGCTGT
CAGCCCGCAACACCTCCCTCAACCCTCAGGAGCTGCAGCGGGAGCGGCAG
CTGCGGATGCTGGAAGATCTGGGCTTCAAGGACCTCACGCTGCAGCCGCG
GGGCCCTCTGGAGCCAGGCCCCCCAAAGCCCGGGGTCCCCCAGGAACCCG
GACGGGGGCAGCCAGATGCAGTGCCTGAGCCCCCACCGGTGGGCTGGCAG
TGCCCCGGGTGCACCTTCATCAACAAGCCCACGCGGCCTGGCTGTGAGAT
GTGCTGCCGGGCGCGCCCCGAGGCCTACCAGGTCCCCGCCTCATACCAGC
CCGACGAGGAGGAGCGAGCGCGCCTGGCGGGCGAGGAGGAGGCGCTGCGT
CAGTACCAGCAGCGGAAGCAGCAGCAGCAGGAGGGGAACTACCTGCAGCA
CGTCCAGCTGGACCAGAGGAGCCTGGTGCTGAACACGGAGCCCGCCGAGT
GCCCCGTGTGCTACTCGGTGCTGGCGCCCGGCGAGGCCGTGGTGCTGCGT
GAGTGTCTGCACACCTTCTGCAGGGAGTGCCTGCAGGGCACCATCCGCAA
CAGCCAGGAGGCGGAGGTCTCCTGCCCCTTCATTGACAACACCTACTCGT
GCTCGGGCAAGCTGCTGGAGAGGGAGATCAAGGCGCTCCTGACCCCTGAG
GATTACCAGCGATTTCTAGACCTGGGCATCTCCATTGCTGAAAACCGCAG
TGCCTTCAGCTACCATTGCAAGACCCCAGATTGCAAGGGATGGTGCTTCT
TTGAGGATGATGTCAATGAGTTCACCTGCCCTGTGTGTTTCCACGTCAAC
TGCCTGCTCTGCAAGGCCATCCATGAGCAGATGAACTGCAAGGAGTATCA
GGAGGACCTGGCCCTGCGGGCTCAGAACGATGTGGCTGCCCGGCAGACGA
CAGAGATGCTGAAGGTGATGCTGCAGCAGGGCGAGGCCATGCGCTGCCCC
CAGTGCCAGATCGTGGTACAGAAGAAGGACGGCTGCGACTGGATCCGCTG
CACCGTCTGCCACACCGAGATCTGCTGGGTCACCAAGGGCtgagcggccg
c

Amino Acid Sequence

View Sequence

MYPYDVPDYAGSPEFMDEKTKKAEEMALSLTRAVAGGDEQVAMKCAIWLA
EQRVPLSVQLKPEVSPTQDIRLWVSVEDAQMHTVTIWLTVRPDMTVASLK
DMVFLDYGFPPVLQQWVIGQRLARDQETLHSHGVRQNGDSAYLYLLSARN
TSLNPQELQRERQLRMLEDLGFKDLTLQPRGPLEPGPPKPGVPQEPGRGQ
PDAVPEPPPVGWQCPGCTFINKPTRPGCEMCCRARPEAYQVPASYQPDEE
ERARLAGEEEALRQYQQRKQQQQEGNYLQHVQLDQRSLVLNTEPAECPVC
YSVLAPGEAVVLRECLHTFCRECLQGTIRNSQEAEVSCPFIDNTYSCSGK
LLEREIKALLTPEDYQRFLDLGISIAENRSAFSYHCKTPDCKGWCFFEDD
VNEFTCPVCFHVNCLLCKAIHEQMNCKEYQEDLALRAQNDVAARQTTEML
KVMLQQGEAMRCPQCQIVVQKKDGCDWIRCTVCHTEICWVTKG*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Truncation. N-term HA tag. Mammalian expression.

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