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Plasmid
pGEX6P-3-RNF25 C159A
Parent Plasmid
pGEX6P-3
Expressed
GST-RNF25 C159A
DU Number
DU65187
Genbank
NM_022453.3 → NP_071898.2
Tag
GST
Species
Human
Sequence of Insert
View Sequence
gtcgacATGGCGGCGTCTGCGTCTGCAGCTGCAGGGGAGGAGGACTGGGT
CCTTCCCTCTGAAGTTGAAGTATTGGAGTCCATCTATCTAGATGAACTAC
AGGTGATTAAAGGAAATGGCAGAACTTCACCATGGGAGATCTACATCACT
TTGCATCCTGCCACTGCAGAGGACCAGGATTCACAGTATGTCTGCTTCAC
TCTGGTGCTTCAGGTCCCAGCAGAGTATCCCCATGAGGTGCCACAGATCT
CTATCCGAAATCCCCGAGGACTTTCAGATGAACAGATCCACACGATCTTA
CAGGTGCTGGGCCACGTGGCCAAGGCTGGGCTGGGCACTGCCATGCTGTA
TGAACTCATTGAGAAAGGGAAGGAAATTCTCACAGATAACAACATCCCTC
ATGGCCAGTGTGTCATCTGCCTCTATGGTTTCCAGGAGAAGGAGGCCTTT
ACCAAAACACCCTGTTACCACTACTTCCACGCCCACTGCCTTGCTCGGTA
CATCCAGCACATGGAGCAAGAGCTGAAGGCACAAGGACAGGAGCAGGAAC
AGGAACGGCAGCATGCTACAACCAAACAGAAGGCAGTCGGTGTGCAGTGT
CCAGTGTGCAGAGAGCCCCTCGTGTATGATCTTGCCTCACTGAAAGCAGC
CCCTGAACCCCAACAGCCCATGGAGCTGTACCAGCCCAGTGCAGAGAGCT
TGCGCCAGCAAGAAGAACGCAAGCGGCTCTACCAGAGGCAGCAGGAGCGG
GGGGGAATCATTGACCTTGAGGCTGAGCGAAACCGATACTTCATCAGCCT
TCAGCAGCCTCCTGCCCCTGCGGAACCTGAGTCAGCTGTAGATGTCTCCA
AAGGATCCCAACCACCCAGCACCCTTGCAGCAGAACTATCCACCTCACCA
GCCGTCCAATCCACTTTGCCACCTCCTCTGCCTGTGGCGACCCAGCACAT
ATGTGAGAAGATTCCAGGGACCAGGTCAAATCAGCAAAGGTTGGGCGAAA
CCCAGAAAGCTATGCTAGATCCCCCCAAGCCCAGTCGAGGTCCCTGGCGA
CAGCCCGAACGGAGGCACCCAAAGGGAGGGGAGTGCCACGCCCCTAAAGG
TACCCGTGACACCCAGGAACTGCCACCTCCTGAGGGGCCCCTCAAGGAGC
CCATGGACCTAAAGCCAGAACCCCATAGCCAAGGAGTTGAAGGTCCTCCA
CAAGAGAAGGGGCCTGGCAGCTGGCAGGGGCCCCCACCCCGCAGGACTCG
GGACTGTGTTCGCTGGGAGCGCTCTAAAGGCCGGACACCCGGTTCTTCCT
ACCCTCGCCTGCCTCGGGGCCAGGGAGCATACCGGCCTGGTACTCGGAGG
GAGTCCCTGGGCCTGGAATCTAAGGATGGTTCCTAGgcggccgc
CCTTCCCTCTGAAGTTGAAGTATTGGAGTCCATCTATCTAGATGAACTAC
AGGTGATTAAAGGAAATGGCAGAACTTCACCATGGGAGATCTACATCACT
TTGCATCCTGCCACTGCAGAGGACCAGGATTCACAGTATGTCTGCTTCAC
TCTGGTGCTTCAGGTCCCAGCAGAGTATCCCCATGAGGTGCCACAGATCT
CTATCCGAAATCCCCGAGGACTTTCAGATGAACAGATCCACACGATCTTA
CAGGTGCTGGGCCACGTGGCCAAGGCTGGGCTGGGCACTGCCATGCTGTA
TGAACTCATTGAGAAAGGGAAGGAAATTCTCACAGATAACAACATCCCTC
ATGGCCAGTGTGTCATCTGCCTCTATGGTTTCCAGGAGAAGGAGGCCTTT
ACCAAAACACCCTGTTACCACTACTTCCACGCCCACTGCCTTGCTCGGTA
CATCCAGCACATGGAGCAAGAGCTGAAGGCACAAGGACAGGAGCAGGAAC
AGGAACGGCAGCATGCTACAACCAAACAGAAGGCAGTCGGTGTGCAGTGT
CCAGTGTGCAGAGAGCCCCTCGTGTATGATCTTGCCTCACTGAAAGCAGC
CCCTGAACCCCAACAGCCCATGGAGCTGTACCAGCCCAGTGCAGAGAGCT
TGCGCCAGCAAGAAGAACGCAAGCGGCTCTACCAGAGGCAGCAGGAGCGG
GGGGGAATCATTGACCTTGAGGCTGAGCGAAACCGATACTTCATCAGCCT
TCAGCAGCCTCCTGCCCCTGCGGAACCTGAGTCAGCTGTAGATGTCTCCA
AAGGATCCCAACCACCCAGCACCCTTGCAGCAGAACTATCCACCTCACCA
GCCGTCCAATCCACTTTGCCACCTCCTCTGCCTGTGGCGACCCAGCACAT
ATGTGAGAAGATTCCAGGGACCAGGTCAAATCAGCAAAGGTTGGGCGAAA
CCCAGAAAGCTATGCTAGATCCCCCCAAGCCCAGTCGAGGTCCCTGGCGA
CAGCCCGAACGGAGGCACCCAAAGGGAGGGGAGTGCCACGCCCCTAAAGG
TACCCGTGACACCCAGGAACTGCCACCTCCTGAGGGGCCCCTCAAGGAGC
CCATGGACCTAAAGCCAGAACCCCATAGCCAAGGAGTTGAAGGTCCTCCA
CAAGAGAAGGGGCCTGGCAGCTGGCAGGGGCCCCCACCCCGCAGGACTCG
GGACTGTGTTCGCTGGGAGCGCTCTAAAGGCCGGACACCCGGTTCTTCCT
ACCCTCGCCTGCCTCGGGGCCAGGGAGCATACCGGCCTGGTACTCGGAGG
GAGTCCCTGGGCCTGGAATCTAAGGATGGTTCCTAGgcggccgc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMAASASAAAGEED
WVLPSEVEVLESIYLDELQVIKGNGRTSPWEIYITLHPATAEDQDSQYVC
FTLVLQVPAEYPHEVPQISIRNPRGLSDEQIHTILQVLGHVAKAGLGTAM
LYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHAHCLA
RYIQHMEQELKAQGQEQEQERQHATTKQKAVGVQCPVCREPLVYDLASLK
AAPEPQQPMELYQPSAESLRQQEERKRLYQRQQERGGIIDLEAERNRYFI
SLQQPPAPAEPESAVDVSKGSQPPSTLAAELSTSPAVQSTLPPPLPVATQ
HICEKIPGTRSNQQRLGETQKAMLDPPKPSRGPWRQPERRHPKGGECHAP
KGTRDTQELPPPEGPLKEPMDLKPEPHSQGVEGPPQEKGPGSWQGPPPRR
TRDCVRWERSKGRTPGSSYPRLPRGQGAYRPGTRRESLGLESKDGS*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMAASASAAAGEED
WVLPSEVEVLESIYLDELQVIKGNGRTSPWEIYITLHPATAEDQDSQYVC
FTLVLQVPAEYPHEVPQISIRNPRGLSDEQIHTILQVLGHVAKAGLGTAM
LYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHAHCLA
RYIQHMEQELKAQGQEQEQERQHATTKQKAVGVQCPVCREPLVYDLASLK
AAPEPQQPMELYQPSAESLRQQEERKRLYQRQQERGGIIDLEAERNRYFI
SLQQPPAPAEPESAVDVSKGSQPPSTLAAELSTSPAVQSTLPPPLPVATQ
HICEKIPGTRSNQQRLGETQKAMLDPPKPSRGPWRQPERRHPKGGECHAP
KGTRDTQELPPPEGPLKEPMDLKPEPHSQGVEGPPQEKGPGSWQGPPPRR
TRDCVRWERSKGRTPGSSYPRLPRGQGAYRPGTRRESLGLESKDGS*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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