HUWE1 | Ubiquitylation Resource

This reagent and more can be found at our sister site MRC PPU Reagents & Services

Request Now

Plasmid

pcDNA FRT TP GFP HUWE1

Parent Plasmid

HUWE1

Expressed

GFP-HUWE1

DU Number

DU66232

Genbank

NM-031407.6

Tag

GFP

Sequence of Insert

View Sequence

GGATCTATGAAAGTAGACAGGACTAAACTGAAGAAGACACCTACTGAGGCTCCTGCAGACTGCAGAGCCTTAATAGACAAACTCAAAGTTTGTAATGATGAGCAACTTCTCTTGGAACTGCAGCAGATCAAAACATGGAACATTGGAAAGTGCGAGTTATATCACTGGGTGGACCTGTTGGACCGCTTCGATGGAATACTGGCAGATGCTGGACAGACAGTGGAGAATATGTCATGGATGCTCGTATGTGATAGGCCAGAAAGAGAGCAACTGAAAATGCTTCTCTTGGCTGTGTTGAACTTCACAGCCTTGCTCATTGAGTACAGCTTTTCCCGGCATCTGTACAGTTCCATAGAGCATTTGACAACTTTATTGGCTTCCTCTGATATGCAAGTGGTGCTGGCAGTCCTCAATCTCCTATATGTATTTAGCAAAAGATCAAACTACATCACTCGTCTGGGATCTGACAAGAGGACCCCGCTGCTAACTCGGCTACAACATTTGGCAGAGAGCTGGGGTGGAAAGGAGAATGGCTTTGGACTTGCAGAATGTTGCAGAGACTTGCATATGATGAAATATCCACCCAGTGCAACTACACTACACTTTGAATTCTACGCAGATCCTGGGGCCGAGGTCAAAATTGAGAAAAGGACAACTAGTAACACACTACATTATATTCACATAGAGCAACTTGACAAGATTTCAGAAAGCCCTTCTGAAATCATGGAATCTCTTACCAAAATGTACAGCATTCCTAAGGATAAGCAGATGCTGTTATTTACACACATACGACTGGCCCATGGCTTTTCTAATCACAGGAAGCGATTGCAGGCAGTTCAGGCCAGACTGCATGCAATATCTATATTAGTGTATTCCAATGCCTTGCAGGAATCAGCAAACAGTATCTTGTATAATGGCTTGATAGAGGAGTTGGTAGATGTCCTTCAGATAACGGATAAGCAGCTTATGGAGATTAAAGCAGCTTCTTTACGAACATTAACATCAATTGTCCACTTGGAGAGAACTCCCAAACTCAGCAGTATTATTGACTGTACTGGAACTGCCTCCTACCATGGATTTTTGCCAGTGCTTGTAAGGAACTGTATCCAGGCCATGATTGATCCTTCCATGGATCCATACCCTCACCAGTTTGCCACTGCTCTCTTCTCTTTTTTATACCATCTGGCCAGCTACGATGCTGGTGGTGAAGCCTTGGTCTCCTGTGGAATGATGGAAGCCTTATTGAAGGTCATAAAGTTTCTTGGCGATGAACAGGACCAGATAACATTTGTCACCAGAGCCGTCAGAGTGGTTGACCTTATCACCAACCTGGATATGGCAGCTTTTCAATCCCATAGTGGACTTTCTATCTTCATTTATAGACTTGAGCATGAAGTAGATTTGTGCCGAAAAGAATGTCCGTTTGTGATCAAGCCAAAGATCCAGAGACCCAATACTACACAAGAAGGAGAGGAAATGGAAACTGATATGGATGGAGTCCAGTGTATTCCACAACGAGCAGTACTTCTGAAATCCATGTTGAATTTCCTCAAGAAGGCCATCCAAGACCCTACTTTCTCAGATGGCATACGACATGTGATGGATGGTTCTCTGCCTACCTCCCTGAAACACATCATCAGCAATGCAGAATACTATGGCCCATCACTCTTCCTCCTAGCTACTGAAGTGGTGACTGTGTTTGTATTTCAAGAACCATCACTGCTCTCCTCACTCCAGGACAATGGATTGACAGATGTCATGCTGCATGCACTGCTTATCAAAGATGTTCCTGCTACCCGTGAAGTCCTTGGCTCCCTCCCAAATGTATTCAGTGCACTCTGTTTGAATGCCCGAGGTCTTCAGTCTTTTGTTCAGTGTCAGCCTTTTGAACGCCTCTTCAAAGTTCTTCTGTCTCCAGATTACCTCCCAGCCATGCGGAGGAGGAGAAGTTCTGATCCCCTTGGGGATACTGCATCCAACCTGGGGAGTGCTGTCGATGAGCTCATGAGACATCAGCCCACCCTTAAAACAGATGCAACGACTGCCATCATCAAGTTACTTGAAGAAATCTGTAATCTTGGAAGGGACCCCAAATACATCTGTCAGAAGCCATCAATCCAGAAGGCAGATGGCACTGCCACTGCTCCTCCCCCAAGGTCTAATCATGCCGCAGAAGAAGCCTCTAGTGAGGATGAGGAGGAAGAGGAAGTACAGGCCATGCAGAGCTTTAATTCTACCCAGCAAAATGAAACTGAGCCTAATCAGCAGGTTGTTGGTACAGAGGAACGTATTCCTATTCCCCTCATGGATTACATCCTTAATGTGATGAAATTTGCGGAATCTATTCTGAGCAACAATACAACAGATGACCACTGCCAGGAATTTGTGAATCAGAAAGGACTGTTGCCTTTGGTTACCATTTTGGGTCTTCCCAATCTGCCCATTGACTTTCCCACATCTGCTGCCTGTCAGGCTGTTGCAGGTGTCTGCAAATCCATATTGACACTGTCACATGAACCCAAAGTCCTTCAAGAGGGTCTCCTTCAGTTGGACTCCATCCTCTCCTCCCTGGAGCCCTTACACCGCCCCATTGAATCCCCTGGGGGCTCAGTGTTGTTGCGAGAACTGGCTTGCGCAGGCAATGTTGCTGGTGCTACCCTCTCAGCCCAGGCCACACCTCTGCTGCATGCACTCACTGCTGCCCATGCCTACATCATGATGTTTGTTCATACTTGCAGAGTTGGACAGAGTGAAATTCGTTCCATCTCCGTAAACCAGTGGGGCTCTCAATTGGGTCTGAGTGTTTTGAGCAAGCTGAGCCAGTTATACTGTTCCCTGGTGTGGGAAAGCACTGTCCTCCTCTCTCTGTGTACCCCAAACAGCCTACCATCTGGGTGTGAATTTGGCCAGGCAGATATGCAGAAACTGGTTCCAAAGGATGAGAAGGCAGGTACGACCCAGGGTGGAAAAAGATCAGATGGGGAACAGGATGGAGCAGCTGGAAGTATGGATGCTTCTACCCAGGGCTTATTAGAAGGCATTGGGCTAGATGGTGACACATTGGCTCCCATGGAGACAGATGAACCTACTGCTTCAGACTCTAAGGGCAAATCTAAAATCACACCAGCAATGGCTGCCAGAATTAAGCAAATCAAGCCTTTGTTATCAGCTTCCTCCAGATTAGGCCGAGCACTTGCTGAGCTATTTGGACTTCTTGTTAAACTTTGTGTGGGATCTCCTGTCCGCCAGAGAAGGAGCCATCATGCTGCCAGCACCACTACAGCACCGACACCTGCCGCGCGATCAACAGCCTCAGCTCTCACTAAGCTCTTGACTAAGGGGTTATCTTGGCAGCCCCCACCATATACACCTACTCCCCGATTCAGGCTGACATTCTTCATCTGTTCAGTTGGTTTCACATCCCCAATGCTGTTTGATGAGAGGAAGTATCCCTACCACCTCATGCTGCAAAAATTTCTCTGCTCCGGAGGCCACAATGCTCTTTTTGAAACTTTCAACTGGGCTCTGTCCATGGGAGGTAAAGTTCCTGTTTCTGAGGGATTGGAACACTCAGACTTGCCTGATGGCACAGGAGAATTCCTAGATGCCTGGCTTATGCTGGTGGAGAAGATGGTGAATCCCACCACGGTGCTTGAATCTCCACATTCGCTGCCTGCCAAATTGCCTGGAGGTGTCCAGAACTTTCCCCAGTTCAGTGCACTGCGCTTCCTTGTGGTAACTCAGAAAGCAGCCTTTACTTGCATCAAAAACTTATGGAACCGGAAACCCCTGAAGGTATATGGTGGACGAATGGCTGAATCGATGCTGGCCATTCTATGCCACATCCTCCGAGGAGAACCTGTGATTCGAGAGAGACTAAGCAAGGAGAAGGAGGGGTCTCGAGGAGAAGAGGATACAGGGCAAGAGGAAGGTGGCTCCCGCCGGGAACCTCAAGTCAACCAGCAACAACTACAACAGCTCATGGACATGGGCTTCACAAGGGAACATGCAATGGAGGCACTGTTGAACACCAGCACCATGGAGCAGGCCACAGAGTACCTTTTAACCCACCCTCCTCCAATCATGGGAGGAGTTGTTCGGGATCTCAGCATGTCTGAAGAGGACCAGATGATGAGAGCAATTGCTATGTCTCTGGGACAGGATATTCCAATGGATCAAAGGGCAGAGTCACCTGAGGAAGTTGCTTGCCGGAAGGAGGAAGAGGAACGGAAAGCTCGGGAAAAGCAGGAGGAGGAAGAGGCTAAATGTCTAGAGAAGTTCCAGGATGCTGACCCGTTGGAACAAGATGAGCTCCACACTTTCACAGATACTATGTTGCCAGGCTGCTTCCACCTTCTTGATGAGCTGCCGGACACAGTATACCGTGTGTGTGACCTGATCATGACAGCAATCAAACGTAATGGAGCAGATTATCGTGACATGATTCTGAAGCAAGTAGTCAATCAGGTGTGGGAAGCTGCTGATGTATTGATCAAAGCTGCTCTTCCCCTGACAACAAGTGACACAAAAACCGTGTCAGAGTGGATAAGTCAGATGGCCACACTGCCCCAGGCCTCCAATTTGGCTACTAGAATCTTGCTTTTAACGCTACTTTTTGAGGAGTTGAAGCTACCTTGTGCTTGGGTGGTTGAATCAAGTGGCATCCTTAATGTCCTAATCAAACTCTTGGAAGTGGTTCAGCCCTGCCTCCAGGCAGCCAAGGAGCAGAAGGAAGTCCAGACCCCAAAGTGGATCACACCAGTGTTGCTCCTGATTGATTTCTATGAAAAGACAGCCATCTCCTCAAAAAGGAGAGCCCAGATGACTAAGTACCTGCAATCCAACAGCAACAACTGGCGCTGGTTTGATGATCGCTCTGGGCGTTGGTGTAGTTACAGTGCAAGCAACAATAGCACTATTGATTCTGCCTGGAAATCTGGAGAGACAAGCGTGCGATTCACTGCAGGCCGAAGAAGATACACGGTCCAATTCACTACAATGGTGCAGGTTAATGAGGAAACAGGGAACCGACGCCCTGTGATGCTGACTCTCCTCAGGGTACCTCGGCTGAATAAAAATTCAAAAAACAGCAATGGACAGGAACTAGAGAAGACGCTGGAAGAAAGCAAAGAAATGGATATCAAACGTAAAGAAAATAAAGGCAATGATACCCCTTTGGCCCTAGAGAGTACAAACACTGAAAAGGAGACAAGCCTGGAGGAAACAAAAATCGGGGAGATCCTGATCCAGGGCTTGACAGAAGATATGGTGACTGTTTTAATCCGGGCCTGCGTGAGCATGCTGGGAGTCCCTGTGGACCCAGATACTTTGCATGCCACCCTTCGTCTCTGTCTGAGGCTCACCCGGGACCACAAATATGCCATGATGTTTGCAGAACTGAAGAGTACCCGCATGATCTTGAATTTGACCCAGAGCTCAGGCTTCAATGGGTTTACTCCCCTGGTCACCCTTCTCTTAAGACACATCGTTGAGGACCCCTGTACCCTTCGTCATACCATGGAAAAGGTCGTTCGCTCAGCAGCTACAAGTGGAGCTGGTAGCACTACCTCTGGTGTTGTGTCTGGCAGCCTCGGCTCTCGGGAGATCAACTACATCCTTCGTGTCCTTGGGCCAGCCGCATGCCGCAATCCAGACATATTCACAGAAGTGGCCAACTGCTGTATCCGCATCGCCCTTCCTGCCCCTCGAGGCTCAGGAACTGCTTCAGATGATGAATTTGAGAATCTTAGAATTAAAGGCCCTAATGCTGTACAGCTGGTGAAGACCACCCCTTTGAAGCCCTCACCTCTGCCTGTCATCCCTGATACTATCAAGGAAGTGATCTATGATATGCTGAATGCTCTGGCTGCATACCATGCTCCAGAGGAAGCAGATAAATCTGATCCTAAACCTGGGGTTATGACCCAAGAGGTTGGCCAGCTCCTGCAAGACATGGGTGATGATGTATACCAGCAGTACCGGTCACTTACGCGTCAGAGCAGTGACTTTGATACGCAGTCAGGTTTTTCCATTAATAGTCAGGTCTTTGCTGCAGATGGTGCCTCCACTGAGACTTCCGCATCTGGGACCTCCCAAGGAGAGGCTTCAACTCCAGAGGAGTCTCGAGATGGGAAGAAAGATAAAGAAGGGGACCGGGCCTCTGAGGAAGGCAAACAGAAAGGCAAGGGCAGCAAACCTTTAATGCCTACCTCCACTATCCTTCGTCTTCTGGCAGAGTTGGTGAGGTCCTATGTTGGTATTGCTACCCTGATTGCCAACTACAGCTACACTGTGGGCCAGTCTGAACTGATCAAAGAGGACTGCAGTGTGCTAGCTTTTGTTCTGGACCACCTGCTCCCACATACCCAGAATGCAGAAGACAAGGACACCCCTGCCTTGGCCCGCCTGTTCCTCGCAAGCCTGGCTGCTGCAGGGAGTGGCACAGATGCCCAGGTGGCCCTAGTGAATGAAGTAAAAGCAGCCCTTGGACGGGCACTGGCTATGGCTGAGAGTACAGAGAAACATGCCAGGCTTCAGGCAGTGATGTGTATCATCAGTACTATCATGGAGTCCTGCCCCTCCACCTCCAGCTTCTACAGCAGTGCCACAGCGAAGACCCAGCACAATGGCATGAACAACATCATTCGGCTTTTCCTGAAGAAGGGACTGGTTAATGACCTGGCCAGAGTACCTCACAGCTTAGACCTGTCCAGTCCCAACATGGCCAACACAGTCAATGCTGCTCTGAAGCCTTTGGAAACACTTTCCCGGATTGTGAACCAGCCCAGTAGCCTTTTTGGCAGCAAGAGTGCTTCTAGCAAGAACAAGTCTGAGCAGGATGCCCAAGGAGCCTCTCAAGATTCCAGTAGCAACCAGCAGGACCCAGGCGAGCCTGGGGAAGCAGAAGTGCAGGAGGAGGATCATGATGTCACTCAGACAGAGGTGGCAGATGGGGATATCATGGATGGGGAGGCTGAAACCGACTCAGTGGTGATTGCTGGGCAGCCTGAGGTGCTCAGTTCACAAGAGATGCAGGTTGAGAATGAGCTGGAGGACCTGATAGATGAGTTGCTTGAGAGGGATGGCGGATCTGGGAACAGTACAATTATAGTGAGCAGAAGTGGAGAGGATGAATCACAAGAGGACGTGCTGATGGATGAAGCTCCTTCCAACCTCAGCCAAGCTTCCACCTTGCAGGCCAACCGAGAAGATTCCATGAATATCCTGGACCCTGAGGATGAGGAGGAGCACACTCAGGAAGAGGACAGCAGTGGCAGTAACGAGGATGAGGATGATAGTCAGCATGAAGAGGAGGAGGAGGAGGAAGATGAGGAAGATGATCAGGAGGATGATGAAGGTGAAGAGGGAGATGAAGACGATGACGACGATGGCTCTGAGATGGAATTGGATGAGGATTATCCTGATATGAACGCTTCTCCCTTGGTCCGATTTGAGCGCTTTGACCGGGAGGATGATCTCATCATTGAGTTTGACAACATGTTCTCCAGTGCTACAGACATCCCCCCATCCCCAGGAAATATCCCTACCACCCATCCACTGATGGTGTGCCATGCAGACCACAGTTCTCTGACACTGGGCAGTGGCTCTTCAACAACTCGTCTCACCCAGGGCATCGGGCGCAGTCAGAGGACCCTAAGGCAGCTGACGGCCAATACTGGCCACACCATTCATGTTCACTACCCTGGGAATCGCCAGCCCAACCCTCCTCTTATACTGCAGAGGTTGCTTGGTCCCTCAGCTGCTGCTGACATCCTTCAGCTGAGCAGCAGCCTTCCCCTACAAAGCCGGGGTCGGGCCCGCCTCCTGGTAGGCAACGATGACGTCCACATCATCGCCCGTTCTGATGATGAGCTGCTGGATGACTTTTTCCATGATCAGAGCACAGCTACCAGCCAAGCAGGAACCCTGTCCAGCATCCCCACAGCCCTGACCCGCTGGACAGAAGAATGCAAAGTTCTCGATGCTGAGAGCATGCATGACTGTGTTTCAGTGGTTAAAGTGTCCATTGTCAATCACCTGGAATTCCTGAGGGATGAGGAGCTGGAAGAAAGGCGAGAGAAGCGCAGGAAACAACTGGCTGAGGAAGAAACAAAGATAACTGATAAAGGCAAAGAAGATAAGGAGAACAGGGATCAGAGTGCACAGTGTACTGCATCTAAGTCAAATGACTCCACTGAACAGAATCTCTCAGATGGGACGCCTATGCCTGACAGCTACCCAACAACCCCATCTTCAACTGATGCAGCTACATCTGAGTCCAAGGAGACCCTTGGCACTCTGCAATCCTCACAACAGCAACCAACACTCCCAACCCCACCAGCTTTGGGAGAGGTTCCTCAGGAGCTGCAGTCTCCAGCTGGAGAAGGGGGCAGCTCTACACAGCTATTGATGCCTGTAGAGCCAGAGGAATTGGGTCCCACAAGGCCAAGTGGGGAAGCAGAAACAACTCAGATGGAGTTATCCCCAGCTCCCACTATAACCTCACTTTCCCCAGAGAGAGCTGAGGATTCTGATGCACTGACGGCTGTCAGCAGTCAGCTAGAAGGCTCTCCTATGGATACAAGCAGCCTGGCTTCCTGTACCTTAGAGGAGGCTGTGGGTGACACTTCAGCAGCTGGCAGTTCTGAGCAGCCCAGAGCAGGCAGCTCCACTCCTGGGGATGCCCCACCAGCTGTGGCGGAAGTGCAAGGCAGGAGTGATGGGTCAGGGGAATCTGCCCAGCCACCTGAGGACAGCTCCCCACCTGCATCCTCTGAGAGCTCTTCCACCAGAGATTCTGCCGTGGCCATTTCTGGAGCAGATTCCCGAGGAATCCTAGAAGGGCCGTTGCCTTCAACAAGCAGTGAAGAAGAAGATCCCCTTGCGGGTATCAGTCTCCCTGAAGGTGTGGACCCCTCTTTTCTGGCTGCCCTGCCTGATGACATCCGTCGGGAAGTTCTACAGAACCAGCTAGGCATTCGTCCACCAACCCGGACTGCCCCCTCCACAAATAGCTCAGCGCCTGCAGTGGTGGGGAATCCTGGTGTGACTGAAGTGAGCCCTGAGTTTCTGGCTGCCCTGCCTCCAGCCATTCAGGAGGAAGTACTGGCACAGCAGAGAGCTGAGCAGCAGCGACGAGAACTAGCACAGAATGCCAGCTCAGACACCCCTATGGACCCTGTGACCTTCATCCAGACTCTGCCCTCAGACCTGCGCCGTAGTGTCCTAGAGGATATGGAGGACAGTGTGTTAGCTGTGATGCCACCTGACATTGCAGCTGAGGCTCAAGCCCTGAGACGAGAGCAAGAAGCCCGGCAGCGACAGCTCATGCATGAGCGTCTGTTTGGGCACAGTAGCACCTCCGCACTCTCTGCTATTCTCCGAAGCCCGGCTTTCACCAGTCGCTTAAGTGGCAACCGTGGGGTCCAGTATACTCGCCTTGCTGTGCAGAGAGGTGGCACCTTCCAGATGGGGGGTAGCAGCAGCCATAACAGGCCTTCTGGCAGTAATGTAGATACTCTCCTCCGCCTCCGAGGACGGCTCCTTCTGGACCACGAAGCCCTTTCTTGTCTCTTGGTCCTACTTTTTGTGGATGAGCCAAAGCTCAATACTAGCCGTCTACACCGAGTACTGAGAAATCTCTGCTACCATGCCCAGACCCGCCACTGGGTCATCCGCAGTCTGCTCTCCATCTTGCAGCGCAGCAGTGAGAGTGAGCTATGCATTGAAACACCCAAACTCACTACAAGTGAGGAAAAGGGCAAAAAGTCGAGCAAGAGCTGTGGGTCAAGTAGCCATGAGAACCGTCCCCTGGACCTGCTACACAAGATGGAGTCAAAGAGCTCCAACCAGCTTTCCTGGCTCTCAGTATCCATGGATGCAGCCCTAGGCTGCAGGACTAATATATTTCAGATCCAGCGTTCAGGGGGGCGTAAACATACCGAGAAGCATGCAAGCGGTGACTCCACCGTCCACATCCATCCCCAAGCTGCACCTGTTGTCTGCAGACACGTTTTGGATACACTCATTCAATTGGCCAAGGTATTTCCCAGCCACTTCACACAGCAGCGGACCAAAGAAACAAACTGTGAGAGTGATCGGGAAAGGGGCAATAAGGCCTGTAGCCCATGCTCCTCACAGTCCTCCAGCAGTGGCATTTGCACAGACTTCTGGGACTTATTGGTAAAACTGGACAACATGAATGTCAGCCGGAAAGGCAAGAACTCCGTGAAGTCAGTGCCAGTGAGCGCTGGCGGTGAGGGGGAAACCTCTCCATACAGCCTCGAGGCCTCTCCACTGGGGCAGCTCATGAACATGTTGTCACACCCAGTCATCCGCCGGAGCTCTCTCTTAACTGAGAAACTCCTCAGACTCCTTTCTCTCATCTCAATTGCTCTCCCAGAAAACAAGGTGTCAGAAGCACAGGCTAATTCTGGCAGCGGTGCTTCCTCCACCACCACTGCCACCTCAACCACATCTACCACCACCACCACTGCCGCCTCCACCACGCCCACACCCCCTACTGCACCCACCCCTGTCACTTCTGCTCCAGCCCTGGTTGCTGCCACGGCTATTTCCACCATTGTCGTAGCTGCTTCGACCACAGTGACTACCCCCACGACTGCTACCACTACTGTTTCAATTTCTCCCACTACTAAGGGCAGCAAATCTCCAGCGAAGGTGAGTGATGGGGGCAGCAGCAGTACAGACTTTAAGATGGTGTCCTCTGGCCTCACTGAAAACCAGCTACAGCTCTCTGTAGAGGTGTTGACATCCCACTCTTGTTCTGAGGAAGGCTTAGAGGATGCAGCCAACGTACTACTGCAGCTCTCCCGGGGGGACTCTGGGACCCGGGACACTGTTCTCAAGCTGCTACTGAATGGAGCCCGCCATCTGGGTTATACCCTTTGTAAACAAATAGGTACCCTGCTGGCCGAGCTGCGGGAATACAACCTCGAGCAGCAGCGGCGAGCCCAATGTGAAACCCTCTCTCCTGATGGCCTGCCTGAGGAGCAGCCACAGACCACCAAGCTGAAGGGCAAAATGCAGAGCAGGTTTGACATGGCTGAGAATGTGGTAATTGTGGCATCTCAGAAGCGACCTTTGGGTGGCCGGGAGCTCCAGCTGCCTTCTATGTCCATGTTGACATCCAAGACATCTACCCAGAAGTTCTTCTTGAGGGTACTACAGGTCATCATCCAGCTCCGGGACGACACGCGCCGGGCTAACAAGAAAGCCAAGCAGACAGGCAGGCTAGGTTCCTCCGGTTTAGGCTCAGCTAGCAGCATCCAGGCAGCTGCTCGGCAGCTGGAGGCTGAGGCTGATGCCATTATACAAATGGTACGTGAGGGTCAAAGGGCGCGGAGACAGCAACAAGCAGCAACGTCGGAGTCTAGCCAGTCAGAGGCGTCTGTCCGGAGGGAGGAATCACCCATGGATGTGGACCAGCCATCTCCCAGTGCTCAAGATACTCAATCCATTGCCTCCGATGGAACCCCACAGGGGGAGAAGGAAAAGGAAGAAAGACCACCTGAGTTACCCCTGCTCAGCGAGCAGCTGAGTTTGGACGAGCTGTGGGACATGCTTGGGGAGTGTCTAAAGGAACTAGAGGAATCCCATGACCAGCATGCGGTGCTAGTGCTACAGCCTGCTGTCGAGGCCTTCTTTCTGGTCCATGCCACAGAGCGGGAGAGCAAGCCTCCTGTCCGAGACACCCGTGAGAGCCAGCTGGCACACATCAAGGACGAGCCTCCTCCACTCTCCCCTGCCCCCTTAACCCCAGCCACGCCTTCCTCCCTTGACCCATTCTTCTCCCGGGAGCCCTCATCTATGCACATCTCCTCAAGCCTGCCCCCTGACACACAGAAGTTCCTTCGCTTTGCAGAGACTCACCGCACTGTGTTAAACCAGATCCTACGGCAGTCCACGACCCACCTTGCTGATGGGCCTTTTGCTGTCCTGGTAGACTACATTCGTGTCCTCGACTTTGATGTCAAGCGCAAATATTTCCGCCAAGAGCTGGAGCGTTTAGATGAGGGGCTCCGGAAAGAAGACATGGCTGTGCATGTCCGTCGTGACCATGTGTTTGAAGACTCCTATCGTGAGCTGCATCGCAAATCCCCCGAAGAAATGAAGAATCGATTGTATATAGTATTTGAAGGAGAAGAAGGGCAGGATGCTGGCGGGCTCCTGCGGGAGTGGTATATGATCATCTCTCGAGAGATGTTTAACCCTATGTATGCCTTGTTCCGTACCTCACCTGGTGATCGAGTCACCTACACCATCAATCCATCTTCCCACTGCAACCCCAACCACCTCAGCTACTTCAAGTTTGTCGGACGCATTGTGGCCAAAGCTGTATATGACAACCGTCTTCTGGAGTGCTACTTTACTCGATCCTTTTACAAACACATCTTGGGCAAGTCAGTCAGATATACAGATATGGAGAGTGAAGATTACCACTTCTACCAAGGTCTGGTTTATCTGCTGGAAAATGATGTCTCCACACTAGGCTATGACCTCACCTTCAGCACTGAGGTCCAAGAGTTTGGAGTTTGTGAAGTTCGTGACCTCAAACCCAATGGGGCCAACATCTTGGTAACAGAGGAGAATAAGAAGGAGTATGTACACCTGGTATGCCAGATGAGAATGACAGGAGCCATCCGCAAGCAGTTGGCGGCTTTCTTAGAAGGCTTCTATGAGATCATTCCAAAGCGCCTCATTTCCATCTTCACTGAGCAGGAGTTAGAGCTGCTTATATCAGGACTGCCCACCATTGACATCGATGATCTGAAATCCAACACTGAATACCACAAGTACCAGTCCAACTCTATTCAGATCCAGTGGTTCTGGAGAGCATTGCGTTCTTTCGATCAAGCTGACCGTGCCAAGTTCCTCCAGTTTGTCACGGGTACTTCCAAGGTACCCCTGCAAGGCTTTGCTGCCCTCGAAGGCATGAATGGCATTCAGAAGTTTCAGATCCATCGAGATGACAGGTCCACAGATCGCCTGCCTTCAGCTCACACATGTTTTAATCAGCTGGATCTGCCTGCCTATGAGAGCTTTGAGAAGCTCCGCCACATGCTACTGTTGGCTATCCAGGAGTGCTCTGAAGGCTTTGGGCTGGCCTAGGCGGCCGC

Amino Acid Sequence

View Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEGEEMETDMDGVQCIPQRAVLLKSMLNFLKKAIQDPTFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFAESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVAGATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGKRSDGEQDGAAGSMDASTQGLLEGIGLDGDTLAPMETDEPTASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIVEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEAEVQEEDHDVTQTEVADGDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDGGSGNSTIIVSRSGEDESQEDVLMDEAPSNLSQASTLQANREDSMNILDPEDEEEHTQEEDSSGSNEDEDDSQHEEEEEEEDEEDDQEDDEGEEGDEDDDDDGSEMELDEDYPDMNASPLVRFERFDREDDLIIEFDNMFSSATDIPPSPGNIPTTHPLMVCHADHSSLTLGSGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKSNDSTEQNLSDGTPMPDSYPTTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCTLEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDGSGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEGPLPSTSSEEEDPLAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESELCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGDSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRTKETNCESDRERGNKACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSAGGEGETSPYSLEASPLGQLMNMLSHPVIRRSSLLTEKLLRLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPTAPTPVTSAPALVAATAISTIVVAASTTVTTPTTATTTVSISPTTKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAARQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Fragment M1-P337 cut Bgl/BamHI and ligated into 66167 to create final product, GROW AT 30C (14kb insert) Please express GFP-HUWE1 in FRT/TO 5.1 expression
Changes from NM_031407.6 CDS: C506V A523T V777A D882G I1828V R2521C E2946G G3319D V3765A

100042 R 1000 1001 1047 19007

Download datasheet