USP43 | Ubiquitylation Resource

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Plasmid

pcDNA FRT TO HA USP43 BirA R118G

Expressed

HA USP43 BirA R118G

DU Number

DU59767

Tag

BirA

Sequence of Insert

View Sequence

GAATTCATGGACCTGGGCCCCGGGGACGCGGCAGGAGGGGGACCGCTCGCGCCCCGGCCCCGCCGCCGCCGCTCCCTGCGCCGCCTGTTCAGCCGCTTCCTGCTGGCGCTGGGCAGCCGCTCACGCCCCGGGGACTCACCGCCCCGGCCCCAGCCGGGACACTGTGATGGCGACGGTGAGGGGGGCTTCGCCTGCGCCCCGGGCCCAGTTCCAGCGGCCCCCGGGAGCCCCGGGGAGGAACGCCCGCCCGGACCCCAGCCCCAGCTCCAGCTCCCCGCCGGCGATGGGGCTCGGCCTCCGGGCGCTCAGGGCTTGAAGAACCACGGCAACACCTGTTTCATGAACGCGGTGGTGCAGTGTCTCAGCAACACCGACCTGCTGGCCGAGTTCCTGGCGCTGGGGCGCTACCGGGCGGCTCCGGGCCGCGCCGAGGTCACCGAGCAGCTGGCGGCGCTGGTGCGCGCGCTCTGGACTCGCGAATACACGCCCCAACTTTCCGCGGAGTTCAAGAATGCAGTTTCCAAGTACGGCTCTCAGTTCCAAGGCAATTCCCAGCACGACGCCCTGGAGTTCCTGCTCTGGTTGCTGGATCGTGTACATGAGGACCTGGAGGGTTCATCCCGAGGGCCGGTGTCGGAGAAGCTTCCGCCTGAAGCCACTAAAACCTCTGAGAACTGCCTGTCACCATCAGCTCAGCTTCCTCTAGGTCAAAGCTTTGTGCAAAGCCACTTTCAAGCACAATATAGATCTTCCTTGACTTGTCCCCACTGCCTGAAACAGAGCAACACCTTTGATCCTTTCCTGTGTGTGTCCCTACCTATCCCCTTGCGCCAGACGAGGTTCTTGAGTGTCACCTTGGTCTTCCCCTCTAAGAGCCAGCGGTTCCTGCGGGTTGGCCTGGCCGTGCCGATCCTCAGCACAGTGGCAGCCCTGAGGAAGATGGTTGCAGAGGAAGGAGGCGTCCCTGCAGATGAGGTGATCTTGGTTGAACTGTATCCCAGTGGATTCCAGCGGTCTTTCTTTGATGAAGAGGACCTGAATACCATCGCAGAGGGAGATAATGTGTATGCCTTTCAAGTTCCTCCCTCACCCAGCCAGGGGACTCTCTCAGCTCATCCACTGGGTCTGTCGGCCTCCCCACGCCTGGCAGCCCGTGAGGGCCAGCGATTCTCCCTCTCTCTCCACAGTGAGAGCAAGGTGCTAATCCTCTTCTGTAACTTGGTGGGGTCAGGGCAGCAGGCTAGCAGGTTTGGGCCACCCTTCCTGATAAGGGAAGACAGAGCTGTTTCCTGGGCCCAGCTCCAGCAGTCTATCCTCAGCAAGGTCCGCCATCTTATGAAGAGTGAGGCCCCTGTACAGAACCTGGGGTCTCTGTTCTCCATCCGTGTTGTGGGACTCTCTGTGGCCTGCAGCTATTTGTCTCCGAAGGACAGTCGGCCCCTCTGTCACTGGGCAGTTGACAGGGTTTTGCATCTCAGGAGGCCAGGAGGCCCTCCACATGTCAAGCTGGCGGTGGAGTGGGATAGCTCTGTCAAGGAGCGCCTGTTCGGGAGCCTCCAGGAGGAGCGAGCGCAGGATGCCGACAGTGTGTGGCAGCAGCAGCAGGCGCATCAGCAGCACAGCTGTACCTTGGATGAATGTTTTCAGTTCTACACCAAGGAGGAGCAGCTGGCCCAGGATGACGCCTGGAAGTGTCCTCACTGCCAAGTCCTGCAGCAGGGGATGGTGAAGCTGAGTTTGTGGACGCTGCCTGACATCCTCATCATCCACCTCAAAAGGTTCTGCCAGGTGGGCGAGAGAAGAAACAAGCTCTCCACGCTGGTGAAGTTTCCGCTCTCTGGACTCAACATGGCTCCCCATGTGGCCCAGAGAAGCACCAGCCCTGAGGCAGGACTGGGCCCCTGGCCTTCCTGGAAGCAGCCGGACTGCCTGCCCACCAGTTACCCGCTGGACTTCCTGTACGACCTGTATGCCGTCTGCAACCACCATGGCAACCTGCAAGGTGGGCATTACACAGCCTACTGCCGGAACTCTCTGGATGGCCAGTGGTACAGTTATGATGACAGCACGGTGGAACCGCTTCGAGAAGATGAGGTCAACACCAGAGGGGCTTATATCCTGTTCTATCAGAAGCGGAACAGCATCCCTCCCTGGTCAGCCAGCAGCTCCATGAGAGGCTCTACCAGCTCCTCCCTGTCTGATCACTGGCTCTTACGGCTCGGGAGCCACGCTGGCAGCACAAGGGGAAGCCTGCTGTCCTGGAGCTCTGCCCCCTGCCCCTCCCTGCCCCAGGTTCCTGACTCTCCCATCTTCACCAACAGCCTCTGCAATCAGGAAAAGGGAGGGTTGGAGCCCAGGCGTTTGGTACGGGGCGTGAAAGGCAGAAGCATTAGCATGAAGGCACCCACCACTTCCCGAGCCAAGCAGGGACCATTCAAGACCATGCCTCTGCGGTGGTCCTTTGGATCCAAGGAGAAACCACCAGGTGCCTCCGTCGAGTTGGTGGAGTACTTGGAATCCAGACGAAGACCTCGGTCCACGAGCCAGTCCATTGTGTCGCTGTTGACGGGCACTGCGGGTGAGGATGAGAAGTCAGCATCGCCGAGGTCCAACGTCGCCCTTCCTGCTAACAGCGAAGATGGTGGGCGGGCCATTGAAAGAGGTCCAGCCGGGGTGCCCTGTCCCTCGGCTCAACCCAACCACTGTCTGGCCCCTGGAAACTCAGATGGTCCAAACACAGCAAGGAAACTCAAGGAAAATGCAGGGCAGGACATCAAGCTTCCCAGAAAGTTTGACCTGCCTCTCACTGTGATGCCTTCAGTGGAGCATGAGAAACCAGCTCGACCGGAGGGCCAGAAGGCCATGAACTGGAAGGAGAGCTTCCAGATGGGAAGCAAAAGCAGCCCACCCTCCCCCTATATGGGATTCTCTGGAAACAGCAAAGACAGTCGCCGAGGCACCTCTGAGCTAGACAGACCCCTGCAGGGGACACTCACCCTTCTGAGGTCCGTGTTTCGGAAGAAGGAGAACAGGAGGAATGAGAGGGCAGAGGTCTCTCCACAGGTGCCCCCCGTCTCCCTGGTGAGTGGCGGGCTGAGCCCTGCCATGGACGGGCAGGCTCCAGGCTCACCTCCTGCCCTCAGGATCCCAGAGGGCCTGGCCAGGGGCCTGGGCAGCCGGCTCGAGAGGGATGTCTGGTCAGCCCCCAGCTCTCTCCGCCTCCCTCGTAAAGCCAGCAGGGCCCCGAGAGGCAGTGCACTGGGCATGTCACAAAGGACTGTTCCAGGGGAGCAGGCTTCTTATGGCACCTTTCAGAGAGTCAAATATCACACTCTTTCTTTAGGTCGAAAGAAAACCTTACCGGAGTCCAGCTTTGCGGCCGC

Amino Acid Sequence

View Sequence

MDLGPGDAAGGGPLAPRPRRRRSLRRLFSRFLLALGSRSRPGDSPPRPQPGHCDGDGEGGFACAPGPVPAAPGSPGEERPPGPQPQLQLPAGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRAAPGRAEVTEQLAALVRALWTREYTPQLSAEFKNAVSKYGSQFQGNSQHDALEFLLWLLDRVHEDLEGSSRGPVSEKLPPEATKTSENCLSPSAQLPLGQSFVQSHFQAQYRSSLTCPHCLKQSNTFDPFLCVSLPIPLRQTRFLSVTLVFPSKSQRFLRVGLAVPILSTVAALRKMVAEEGGVPADEVILVELYPSGFQRSFFDEEDLNTIAEGDNVYAFQVPPSPSQGTLSAHPLGLSASPRLAAREGQRFSLSLHSESKVLILFCNLVGSGQQASRFGPPFLIREDRAVSWAQLQQSILSKVRHLMKSEAPVQNLGSLFSIRVVGLSVACSYLSPKDSRPLCHWAVDRVLHLRRPGGPPHVKLAVEWDSSVKERLFGSLQEERAQDADSVWQQQQAHQQHSCTLDECFQFYTKEEQLAQDDAWKCPHCQVLQQGMVKLSLWTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAQRSTSPEAGLGPWPSWKQPDCLPTSYPLDFLYDLYAVCNHHGNLQGGHYTAYCRNSLDGQWYSYDDSTVEPLREDEVNTRGAYILFYQKRNSIPPWSASSSMRGSTSSSLSDHWLLRLGSHAGSTRGSLLSWSSAPCPSLPQVPDSPIFTNSLCNQEKGGLEPRRLVRGVKGRSISMKAPTTSRAKQGPFKTMPLRWSFGSKEKPPGASVELVEYLESRRRPRSTSQSIVSLLTGTAGEDEKSASPRSNVALPANSEDGGRAIERGPAGVPCPSAQPNHCLAPGNSDGPNTARKLKENAGQDIKLPRKFDLPLTVMPSVEHEKPARPEGQKAMNWKESFQMGSKSSPPSPYMGFSGNSKDSRRGTSELDRPLQGTLTLLRSVFRKKENRRNERAEVSPQVPPVSLVSGGLSPAMDGQAPGSPPALRIPEGLARGLGSRLERDVWSAPSSLRLPRKASRAPRGSALGMSQRTVPGEQASYGTFQRVKYHTLSLGRKKTLPESSFAAAMKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Please clone DU50016 with BirA R118G at C terminus
Thanks

100042 R 1000 1001 1047 19007

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