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Plasmid
pGEX6P-2-HOIL1 C460A C506A
Parent Plasmid
pGEX6P-2
Expressed
GST-HOIL1 [Cys460Ala Cys506Ala]
DU Number
DU29737
Genbank
Q9BYM8
Tag
GST
Species
Human
Sequence of Insert
View Sequence
gcggccgcgatggacgagaagaccaagaaagcagaggaaatggccctgag
cctcacccgagcagtggcgggcggggatgaacaggtggcaatgaagtgtg
ccatctggctggcagagcaacgggtgcccctgagtgtgcaactgaagcct
gaggtctccccaacgcaggacatcaggctgtgggtgagcgtggaggatgc
tcagatgcacaccgtcaccatctggctcacagtgcgccctgatatgacag
tggcgtctctcaaggacatggtttttctggactatggcttcccaccagtc
ttgcagcagtgggtgattgggcagcggctggcacgagaccaggagaccct
gcactcccatggggtgcggcagaatggggacagtgcctacctctatctgc
tgtcagcccgcaacacctccctcaaccctcaggagctgcagcgggagcgg
cagctgcggatgctggaagatctgggcttcaaggacctcacgctgcagcc
gcggggccctctggagccaggccccccaaagcccggggtcccccaggaac
ccggacgggggcagccagatgcagtgcctgagcccccaccggtgggctgg
cagtgccccgggtgcaccttcatcaacaagcccacgcggcctggctgtga
gatgtgctgccgggcgcgccccgaggcctaccaggtccccgcctcatacc
agcccgacgaggaggagcgagcgcgcctggcgggcgaggaggaggcgctg
cgtcagtaccagcagcggaagcagcagcagcaggaggggaactacctgca
gcacgtccagctggaccagaggagcctggtgctgaacacggagcccgccg
agtgccccgtgtgctactcggtgctggcgcccggcgaggccgtggtgctg
cgtgagtgtctgcacaccttctgcagggagtgcctgcagggcaccatccg
caacagccaggaggcggaggtctcctgccccttcattgacaacacctact
cgtgctcgggcaagctgctggagagggagatcaaggcgctcctgacccct
gaggattaccagcgatttctagacctgggcatctccattgctgaaaaccg
cagtgccttcagctaccattgcaagaccccagattgcaagggatggtgct
tctttgaggatgatgtcaatgagttcacctgccctgtgtgtttccacgtc
aactgcctgctctgcaaggccatccatgagcagatgaactgcaaggagta
tcaggaggacctggccctgcgggctcagaacgatgtggctgcccggcaga
cgacagagatgctgaaggtgatgctgcagcagggcgaggccatgcgctgc
ccccagtgccagatcgtggtacagaagaaggacggcGCcgactggatccg
ctgcaccgtctgccacaccgagatctgctgggtcaccaagggcccacgct
ggggccctgggggcccaggagacaccagcgggggctgccgctgcagggta
aatgggattccttgccacccaagcGCtcagaactgccactgagcggccgc
cctcacccgagcagtggcgggcggggatgaacaggtggcaatgaagtgtg
ccatctggctggcagagcaacgggtgcccctgagtgtgcaactgaagcct
gaggtctccccaacgcaggacatcaggctgtgggtgagcgtggaggatgc
tcagatgcacaccgtcaccatctggctcacagtgcgccctgatatgacag
tggcgtctctcaaggacatggtttttctggactatggcttcccaccagtc
ttgcagcagtgggtgattgggcagcggctggcacgagaccaggagaccct
gcactcccatggggtgcggcagaatggggacagtgcctacctctatctgc
tgtcagcccgcaacacctccctcaaccctcaggagctgcagcgggagcgg
cagctgcggatgctggaagatctgggcttcaaggacctcacgctgcagcc
gcggggccctctggagccaggccccccaaagcccggggtcccccaggaac
ccggacgggggcagccagatgcagtgcctgagcccccaccggtgggctgg
cagtgccccgggtgcaccttcatcaacaagcccacgcggcctggctgtga
gatgtgctgccgggcgcgccccgaggcctaccaggtccccgcctcatacc
agcccgacgaggaggagcgagcgcgcctggcgggcgaggaggaggcgctg
cgtcagtaccagcagcggaagcagcagcagcaggaggggaactacctgca
gcacgtccagctggaccagaggagcctggtgctgaacacggagcccgccg
agtgccccgtgtgctactcggtgctggcgcccggcgaggccgtggtgctg
cgtgagtgtctgcacaccttctgcagggagtgcctgcagggcaccatccg
caacagccaggaggcggaggtctcctgccccttcattgacaacacctact
cgtgctcgggcaagctgctggagagggagatcaaggcgctcctgacccct
gaggattaccagcgatttctagacctgggcatctccattgctgaaaaccg
cagtgccttcagctaccattgcaagaccccagattgcaagggatggtgct
tctttgaggatgatgtcaatgagttcacctgccctgtgtgtttccacgtc
aactgcctgctctgcaaggccatccatgagcagatgaactgcaaggagta
tcaggaggacctggccctgcgggctcagaacgatgtggctgcccggcaga
cgacagagatgctgaaggtgatgctgcagcagggcgaggccatgcgctgc
ccccagtgccagatcgtggtacagaagaaggacggcGCcgactggatccg
ctgcaccgtctgccacaccgagatctgctgggtcaccaagggcccacgct
ggggccctgggggcccaggagacaccagcgggggctgccgctgcagggta
aatgggattccttgccacccaagcGCtcagaactgccactgagcggccgc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMDEKTKKA
EEMALSLTRAVAGGDEQVAMKCAIWLAEQRVPLSVQLKPEVSPTQDIRLW
VSVEDAQMHTVTIWLTVRPDMTVASLKDMVFLDYGFPPVLQQWVIGQRLA
RDQETLHSHGVRQNGDSAYLYLLSARNTSLNPQELQRERQLRMLEDLGFK
DLTLQPRGPLEPGPPKPGVPQEPGRGQPDAVPEPPPVGWQCPGCTFINKP
TRPGCEMCCRARPEAYQVPASYQPDEEERARLAGEEEALRQYQQRKQQQQ
EGNYLQHVQLDQRSLVLNTEPAECPVCYSVLAPGEAVVLRECLHTFCREC
LQGTIRNSQEAEVSCPFIDNTYSCSGKLLEREIKALLTPEDYQRFLDLGI
SIAENRSAFSYHCKTPDCKGWCFFEDDVNEFTCPVCFHVNCLLCKAIHEQ
MNCKEYQEDLALRAQNDVAARQTTEMLKVMLQQGEAMRCPQCQIVVQKKD
GADWIRCTVCHTEICWVTKGPRWGPGGPGDTSGGCRCRVNGIPCHPSAQN
CH*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMDEKTKKA
EEMALSLTRAVAGGDEQVAMKCAIWLAEQRVPLSVQLKPEVSPTQDIRLW
VSVEDAQMHTVTIWLTVRPDMTVASLKDMVFLDYGFPPVLQQWVIGQRLA
RDQETLHSHGVRQNGDSAYLYLLSARNTSLNPQELQRERQLRMLEDLGFK
DLTLQPRGPLEPGPPKPGVPQEPGRGQPDAVPEPPPVGWQCPGCTFINKP
TRPGCEMCCRARPEAYQVPASYQPDEEERARLAGEEEALRQYQQRKQQQQ
EGNYLQHVQLDQRSLVLNTEPAECPVCYSVLAPGEAVVLRECLHTFCREC
LQGTIRNSQEAEVSCPFIDNTYSCSGKLLEREIKALLTPEDYQRFLDLGI
SIAENRSAFSYHCKTPDCKGWCFFEDDVNEFTCPVCFHVNCLLCKAIHEQ
MNCKEYQEDLALRAQNDVAARQTTEMLKVMLQQGEAMRCPQCQIVVQKKD
GADWIRCTVCHTEICWVTKGPRWGPGGPGDTSGGCRCRVNGIPCHPSAQN
CH*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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