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Plasmid
pCMV5 FLAG1 IRAK2 R485E
Parent Plasmid
pCMV5 FLAG1
Expressed
FLAG IRAK2 R485E
DU Number
DU29707
Genbank
NM_001570.3 GI:58530885
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
ggatccatggcctgctacatctaccagctgccctcctgggtgctggacga
cctgtgccgcaacatggacgcgctcagcgagtgggactggatggagttcg
cctcctacgtgatcacagacctgacccagctgcggaagatcaagtccatg
gagcgggtgcagggtgtgagcatcacgcgggagctgctgtggtggtgggg
catgcggcaggccaccgtccagcaacttgtggacctcctgtgccgcctgg
agctctaccgggctgcccagatcatcctgaactggaaaccggctcctgaa
atcaggtgtcccattccagccttccctgactctgtgaagccagaaaagcc
tttggcagcttctgtaagaaaggctgaggatgaacaggaagaggggcagc
ctgtgaggatggccacctttccaggcccagggtcctctccagccagagcc
caccagccggcctttctccagcctcctgaagaagatgcccctcattcctt
gagaagcgacctccccacttcgtctgattcaaaggacttcagcacctcca
ttcctaagcaggaaaaacttttgagcttggctggagacagccttttctgg
agtgaggcagacgtggtccaggcaaccgatgacttcaatcaaaaccgcaa
aatcagccaggggacctttgctgacgtctacagagggcacaggcacggga
agccattcgtcttcaagaagctcagagagacagcctgttcaagtccagga
tcaatcgaaagattcttccaggcagagttgcagatttgtcttagatgctg
ccaccccaatgtcttacctgtgctgggcttctgtgctgcaagacagtttc
acagcttcatctacccctacatggcaaatggttccctacaggacagactg
cagggtcagggtggctcggaccccctcccctggccccagcgtgtcagcat
ctgctcagggctgctctgtgccgtcgagtacctgcatggtctggagatca
tccacagcaacgtcaagagctctaatgtcttgctggaccaaaatctcacc
cccaaacttgctcacccaatggctcatctgtgtcctgtcaacaaaaggtc
aaaatacaccatgatgaagactcacctgctccggacgtcagccgcgtatc
tgccagaggatttcatccgggtggggcagctgacaaagcgagtggacatc
ttcagctgtggaatagtgttggccgaggtcctcacgggcatccctgcaat
ggataacaaccgaagcccggtttacctgaaggacttactcctcagtgata
ttccaagcagcaccgcctcgctctgctccaggaagacgggcgtggagaac
gtgatggcaaaggagatctgccagaagtacctggagaagggcgcagggag
gcttccggaggactgcgccgaggccctggccacggctgcctgcctgtgcc
tgcggaggGAGaacaccagcctgcaggaggtgtgtggctctgtggctgct
gtggaagagcggctccgaggtcgggagacgttgctcccttggagtgggct
ttctgagggtacaggctcttcttccaacaccccagaggaaacagacgacg
ttgacaattccagccttgatgcctcctcctccatgagtgtggcaccctgg
gcaggggctgccaccccacttctccccacagagaatggggaaggaaggct
gcgggtcatcgtgggaagggaggctgactcctcctctgaggcctgtgttg
gcctggagcctccccaggatgttacagaaacttcgtggcaaattgagatc
aatgaggccaaaaggaaactgatggagaatattctgctctacaaagagga
aaaagtggacagcattgagctctttggcccctgagcggccgc
cctgtgccgcaacatggacgcgctcagcgagtgggactggatggagttcg
cctcctacgtgatcacagacctgacccagctgcggaagatcaagtccatg
gagcgggtgcagggtgtgagcatcacgcgggagctgctgtggtggtgggg
catgcggcaggccaccgtccagcaacttgtggacctcctgtgccgcctgg
agctctaccgggctgcccagatcatcctgaactggaaaccggctcctgaa
atcaggtgtcccattccagccttccctgactctgtgaagccagaaaagcc
tttggcagcttctgtaagaaaggctgaggatgaacaggaagaggggcagc
ctgtgaggatggccacctttccaggcccagggtcctctccagccagagcc
caccagccggcctttctccagcctcctgaagaagatgcccctcattcctt
gagaagcgacctccccacttcgtctgattcaaaggacttcagcacctcca
ttcctaagcaggaaaaacttttgagcttggctggagacagccttttctgg
agtgaggcagacgtggtccaggcaaccgatgacttcaatcaaaaccgcaa
aatcagccaggggacctttgctgacgtctacagagggcacaggcacggga
agccattcgtcttcaagaagctcagagagacagcctgttcaagtccagga
tcaatcgaaagattcttccaggcagagttgcagatttgtcttagatgctg
ccaccccaatgtcttacctgtgctgggcttctgtgctgcaagacagtttc
acagcttcatctacccctacatggcaaatggttccctacaggacagactg
cagggtcagggtggctcggaccccctcccctggccccagcgtgtcagcat
ctgctcagggctgctctgtgccgtcgagtacctgcatggtctggagatca
tccacagcaacgtcaagagctctaatgtcttgctggaccaaaatctcacc
cccaaacttgctcacccaatggctcatctgtgtcctgtcaacaaaaggtc
aaaatacaccatgatgaagactcacctgctccggacgtcagccgcgtatc
tgccagaggatttcatccgggtggggcagctgacaaagcgagtggacatc
ttcagctgtggaatagtgttggccgaggtcctcacgggcatccctgcaat
ggataacaaccgaagcccggtttacctgaaggacttactcctcagtgata
ttccaagcagcaccgcctcgctctgctccaggaagacgggcgtggagaac
gtgatggcaaaggagatctgccagaagtacctggagaagggcgcagggag
gcttccggaggactgcgccgaggccctggccacggctgcctgcctgtgcc
tgcggaggGAGaacaccagcctgcaggaggtgtgtggctctgtggctgct
gtggaagagcggctccgaggtcgggagacgttgctcccttggagtgggct
ttctgagggtacaggctcttcttccaacaccccagaggaaacagacgacg
ttgacaattccagccttgatgcctcctcctccatgagtgtggcaccctgg
gcaggggctgccaccccacttctccccacagagaatggggaaggaaggct
gcgggtcatcgtgggaagggaggctgactcctcctctgaggcctgtgttg
gcctggagcctccccaggatgttacagaaacttcgtggcaaattgagatc
aatgaggccaaaaggaaactgatggagaatattctgctctacaaagagga
aaaagtggacagcattgagctctttggcccctgagcggccgc
Amino Acid Sequence
View Sequence
MDYKDDDDKGSMACYIYQLPSWVLDDLCRNMDALSEWDWMEFASYVITDL
TQLRKIKSMERVQGVSITRELLWWWGMRQATVQQLVDLLCRLELYRAAQI
ILNWKPAPEIRCPIPAFPDSVKPEKPLAASVRKAEDEQEEGQPVRMATFP
GPGSSPARAHQPAFLQPPEEDAPHSLRSDLPTSSDSKDFSTSIPKQEKLL
SLAGDSLFWSEADVVQATDDFNQNRKISQGTFADVYRGHRHGKPFVFKKL
RETACSSPGSIERFFQAELQICLRCCHPNVLPVLGFCAARQFHSFIYPYM
ANGSLQDRLQGQGGSDPLPWPQRVSICSGLLCAVEYLHGLEIIHSNVKSS
NVLLDQNLTPKLAHPMAHLCPVNKRSKYTMMKTHLLRTSAAYLPEDFIRV
GQLTKRVDIFSCGIVLAEVLTGIPAMDNNRSPVYLKDLLLSDIPSSTASL
CSRKTGVENVMAKEICQKYLEKGAGRLPEDCAEALATAACLCLRRENTSL
QEVCGSVAAVEERLRGRETLLPWSGLSEGTGSSSNTPEETDDVDNSSLDA
SSSMSVAPWAGAATPLLPTENGEGRLRVIVGREADSSSEACVGLEPPQDV
TETSWQIEINEAKRKLMENILLYKEEKVDSIELFGP*
TQLRKIKSMERVQGVSITRELLWWWGMRQATVQQLVDLLCRLELYRAAQI
ILNWKPAPEIRCPIPAFPDSVKPEKPLAASVRKAEDEQEEGQPVRMATFP
GPGSSPARAHQPAFLQPPEEDAPHSLRSDLPTSSDSKDFSTSIPKQEKLL
SLAGDSLFWSEADVVQATDDFNQNRKISQGTFADVYRGHRHGKPFVFKKL
RETACSSPGSIERFFQAELQICLRCCHPNVLPVLGFCAARQFHSFIYPYM
ANGSLQDRLQGQGGSDPLPWPQRVSICSGLLCAVEYLHGLEIIHSNVKSS
NVLLDQNLTPKLAHPMAHLCPVNKRSKYTMMKTHLLRTSAAYLPEDFIRV
GQLTKRVDIFSCGIVLAEVLTGIPAMDNNRSPVYLKDLLLSDIPSSTASL
CSRKTGVENVMAKEICQKYLEKGAGRLPEDCAEALATAACLCLRRENTSL
QEVCGSVAAVEERLRGRETLLPWSGLSEGTGSSSNTPEETDDVDNSSLDA
SSSMSVAPWAGAATPLLPTENGEGRLRVIVGREADSSSEACVGLEPPQDV
TETSWQIEINEAKRKLMENILLYKEEKVDSIELFGP*
Vector Type
Mammalian
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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