MINDY2 (KIAA1164)

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Plasmid
pGEX6P1 KIAA1164 P267A Y319A M323A R342A E350A Y351A D359A 241-504
Parent Plasmid
pGEX6P1
Expressed
GST KIAA1164 P267A Y319A M323A R342A E350A Y351A D359A A392E 241-504
DU Number
DU59697
Tag
GST
Sequence of Insert
AGATCTATGTCTGTGTATCACATCAAGTGGATCCAGTGGAAGGAAGAGAACACACCCATCATCACCCAGAATGAGAACGGACCCTGCGCCTTGCTGGCCATCCTCAATGTTTTGCTCCTGGCCTGGAAGGTGAAACTTCCACCGATGATGGAAATCATAACTGCTGAGCAGCTGATGGAATATTTAGGAGATTACATGCTTGATGCAAAGCCAAAAGAAATTTCAGAAATTCAACGTTTAAATGCTGAACAGAATGCGAGTGATGCCATGGCAATTTTGCACAAACTACAGACAGGCCTGGATGTAAATGTAGCATTCACTGGTGTTCGAGTGTTTGCAGCTACACCAGAATGCATAGTATTTGCTCTTCTTGATATTCCTTTGTACCATGGGTGGTTAGTAGACCCTCAGATTGATGACATTGTAAAAGCTGTTGGTAACTGCAGCTACAACCAACTAGTGGAGAAGATCATCTCTTGTAAACAGTCAGACAATAGTGAGCTGGTTAGTGAAGGCTTTGTAGCTGAGCAGTTTCTAAATAACACAGCCACTCAACTGACATACCATGGATTATGTGAACTAACTTCAACGGTTCAGGAAGGAGAACTTTGTGTGTTCTTTCGGAATAATCATTTTAGCACCATGACCAAATACAAGGGTCAACTGTATTTGTTGGTAACGGACCAGGGGTTTCTTACTGAAGAGAAAGTTGTTTGGGAAAGCCTACACAACGTAGATGGTGATGGAAATTTCTGTGACTCAGAATTTCATCTTCGACCTCCTTCAGATCCTGAAACTGTATAAGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSVYHIKWIQWKEENTPIITQNENGPCALLAILNVLLLAWKVKLPPMMEIITAEQLMEYLGDYMLDAKPKEISEIQRLNAEQNASDAMAILHKLQTGLDVNVAFTGVRVFAATPECIVFALLDIPLYHGWLVDPQIDDIVKAVGNCSYNQLVEKIISCKQSDNSELVSEGFVAEQFLNNTATQLTYHGLCELTSTVQEGELCVFFRNNHFSTMTKYKGQLYLLVTDQGFLTEEKVVWESLHNVDGDGNFCDSEFHLRPPSDPETV*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Please mutate P267 to alanine and A392 to glutamate in 59473
100042 R 1000 1001 1047 19007
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