UBD (FAT10) | Ubiquitylation Resource

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Synonyms

Plasmid

pTXB1-FAT10 aa1-162 (C7S C9S C134S C160S C162S)-T3C Mxe intein CBD

Parent Plasmid

pTXB1

Expressed

FAT10 aa1-162 (C7S C9S C134S C160S C162S)-T3C Mxe intein CBD

DU Number

DU29485

Genbank

O15205

Tag

Intein

Species

Human

Sequence of Insert

View Sequence

GCTAGCATGGGTTGCTCTTCCGGCGCTCCCAATGCTTCCTCTCTCTCTGTGCATGTCCGTTCCGAGGAATGGGATTTAATGACCTTTGATGCCAACCCATATGACAGCGTGAAAAAAATCAAAGAACATGTCCGGTCTAAGACCAAGGTTCCTGTGCAGGACCAGGTTCTTTTGCTGGGCTCCAAGATCTTAAAGCCACGGAGAAGCCTCTCATCTTATGGCATTGACAAAGAGAAGACCATCCACCTTACCCTGAAAGTGGTGAAGCCCAGTGATGAGGAGCTGCCCTTGTTTCTTGTGGAGTCAGGTGATGAGGCAAAGAGGCACCTCCTCCAGGTGCGAAGGTCCAGCTCAGTGGCACAAGTGAAAGCAATGATCGAGACTAAGACGGGTATAATCCCTGAGACCCAGATTGTGACTTCCAATGGAAAGAGACTGGAAGATGGGAAGATGATGGCAGATTATGGCATCAGAAAGGGCAACTTACTCTTCCTGGCATCTTATTCTTGCATCTGCGGAGATGCACTAGT

Amino Acid Sequence

View Sequence

MASMGCSSGAPNASSLSVHVRSEEWDLMTFDANPYDSVKKIKEHVRSKTK
VPVQDQVLLLGSKILKPRRSLSSYGIDKEKTIHLTLKVVKPSDEELPLFL
VESGDEAKRHLLQVRRSSSVAQVKAMIETKTGIIPETQIVTSNGKRLEDG
KMMADYGIRKGNLLFLASYSCICGDALVALPEGESVRIADIVPGARPNSD
NAIDLKVLDRHGNPVLADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCL
VDVAGVPTLLWKLIDEIKPGD*

Vector Type

Bacterial

Antibiotic

Amp

Comments

additional N-terminal residues (MAS) derived from translation initiation at the pTXB1 vector derived Nde1 site. Native FAT10 Met1 = Met4 of translation product (with GCSSG inserted between native Met1 and Ala2)
Intein T3C mutant

Contains silent changes at Gly120 of the Mxe/Intein (GGA>GGG) and Tyr148 of FAT10 (TAT>TAC)

PARENTAL 45876

100042 R 1000 1001 1047 19007

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