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UBD (FAT10)

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Plasmid
pSc-B-FAT10 aa1-162 (C7S C9S C160S C162S)-T3C intein overlap
Parent Plasmid
pSC-B
DU Number
DU29457
Genbank
O15205
Species
Human
Sequence of Insert
GCTAGCATGGGTTGCTCTTCCGGCGCTCCCAATGCTTCCTCTCTCTCTGTGCATGTCCGTTCCGAGGAATGGGATTTAATGACCTTTGATGCCAACCCATATGACAGCGTGAAAAAAATCAAAGAACATGTCCGGTCTAAGACCAAGGTTCCTGTGCAGGACCAGGTTCTTTTGCTGGGCTCCAAGATCTTAAAGCCACGGAGAAGCCTCTCATCTTATGGCATTGACAAAGAGAAGACCATCCACCTTACCCTGAAAGTGGTGAAGCCCAGTGATGAGGAGCTGCCCTTGTTTCTTGTGGAGTCAGGTGATGAGGCAAAGAGGCACCTCCTCCAGGTGCGAAGGTCCAGCTCAGTGGCACAAGTGAAAGCAATGATCGAGACTAAGACGGGTATAATCCCTGAGACCCAGATTGTGACTTGCAATGGAAAGAGACTGGAAGATGGGAAGATGATGGCAGATTACGGCATCAGAAAGGGCAACTTACTCTTCCTGGCATCTTATTCTTGCATCTGCGGAGATGCACTAGT
Amino Acid Sequence
MGCSSGAPNASSLSVHVRSEEWDLMTFDANPYDSVKKIKEHVRSKTKVPV
QDQVLLLGSKILKPRRSLSSYGIDKEKTIHLTLKVVKPSDEELPLFLVES
GDEAKRHLLQVRRSSSVAQVKAMIETKTGIIPETQIVTCNGKRLEDGKMM
ADYGIRKGNLLFLASYSCICGDAL
Vector Type
Other
Antibiotic
Amp; Kan
Comments
GCSSG inserted after Met1 and 3' end overlaps intein (to Spe1 site) and includes T3C mutation. No stop. For cloning into Nhe1 Spe1 sites of pTXB1
100042 R 1000 1001 1047 19007
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