CHFR | Ubiquitylation Resource

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Synonyms

E3 ubiquitin-protein ligase CHFR isoform 1, RNF116, RNF196, checkpoint with forkhead and ring finger domains

Plasmid

pGEX6P-1-CHFR isoform 1

Parent Plasmid

pGEX6P-1

Expressed

GST-CHFR isoform 1

DU Number

DU29294

Genbank

NM_001161344.1 → NP_001154816.1

Tag

GST

Species

Human

Sequence of Insert

View Sequence

ggatccATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCC
CTGGGGACGGCTCCTGCGTCTGGGCGCGGAGGAGGGCGAGCCGCACGTCC
TCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGCGACCTT
TCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGT
GGATGAAAAATCAGGTCAGGTGACACTGGAAGATACCAGCACCAGTGGAA
CAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGACATGCCCTTTACAG
ACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAA
CGTGGCATACCTCTATGAATCTTTAAGTGAAAAGCAAGGCATGACACAAG
AATCCTTTGAAGCTAACAAGGAAAATGTGTTCCATGGGACCAAAGATACC
TCAGGTGCAGGTGCAGGGCGAGGGGCCGATCCCCGGGTCCCTCCGTCGTC
GCCCGCCACTCAGGTGTGCTTTGAGGAACCACAGCCATCAACATCGACGT
CAGACCTCTTCCCCACAGCCTCGGCCTCTTCCACGGAGCCTTCTCCTGCA
GGGCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGTGGTGGCATCTCCCC
TAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGTCTCCAGCTTTGCCT
CAGCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGGAACCCCAG
GATCAGGAGGATTTGGAGCCCGTGAAGAAGAAAATGAGAGGAGATGGGGA
CCTTGACCTGAACGGGCAGTTGTTGGTCGCACAACCGCGTAGAAATGCCC
AAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGAAGCCAGACAAGATG
GAGGAGACGCTGACATGCATCATCTGCCAGGACCTGCTGCACGACTGCGT
GAGTTTGCAGCCCTGCATGCACACGTTCTGCGCGGCTTGCTACTCGGGCT
GGATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCTGTCCCGTGGAGCGG
ATCTGTAAAAACCACATCCTCAACAACCTCGTGGAAGCATACCTCATCCA
GCATCCAGACAAGAGTCGCAGTGAAGAAGATGTGCAAAGTATGGATGCCA
GGAATAAAATCACTCAAGACATGCTGCAGCCCAAAGTCAGGCGGTCTTTT
TCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCAGACGTTGA
CAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTC
CTGAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACTGCCCAGCACCCGAG
GGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCACCCTCCACGTCCGT
CAGCCTGACGACAGCAGTCCAGGATTACGTGTGCCCTCTGCAAGGAAGCC
ACGCCCTGTGCACCTGCTGCTTCCAGCCCATGCCCGACCGGAGAGCGGAG
CGCGAGCAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCGGTCTGCCTGCA
GCCTTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGGCTGCTACGGCT
GCCTGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGTGTCTGGACGGC
GTGCTGAACAACAACAGCTACGAGTCAGACATCCTGAAGAATTACCTGGC
AACCAGAGGTTTGACATGGAAAAACATGTTGACCGAGAGCCTCGTGGCTC
TCCAGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCACGGGAGACACC
GTTCTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTGACCTATCA
GTATCGGCAGAACATTCCTGCTTCCGAGTTGCCAGTGGCCGTAACATCCC
GTCCTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGGTGAAAGCTCAC
CACGCCATGAAATTCAATCATATCTGTGAACAGACAAGGTTCAAAAACTA
Agcggccgc

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMERPEEGKQSPPPQPWGRL
LRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKS
GQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYL
YESLSEKQGMTQESFEANKENVFHGTKDTSGAGAGRGADPRVPPSSPATQ
VCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSG
PSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLN
GQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQP
CMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDK
SRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESS
DISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTT
AVQDYVCPLQGSHALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCH
LYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGL
TWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQN
IPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Silent change at R382 (AGA > AGG) compared to NCBI ref

100042 R 1000 1001 1047 19007

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