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CHFR

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Synonyms
E3 ubiquitin-protein ligase CHFR isoform 1, RNF116, RNF196, checkpoint with forkhead and ring finger domains
Plasmid
pGEX6P-1-CHFR isoform 3
Parent Plasmid
pS-B
Expressed
GST-CHFR isoform 3
DU Number
DU29270
Genbank
NM_001161346.1 → NP_001154818.1
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCC
CTGGGGACGGCTCCTGCGTCTGGGCGCGGAGGAGGGCGAGCCGCACGTCC
TCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGCGACCTT
TCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGT
GGATGAAAAATCAGGTCAGGTGACACTGGAAGATACCAGCACCAGTGGAA
CAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGACATGCCCTTTACAG
ACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAA
CGTGGCATACCTCTATGAATCTTTAAGTGAAAAGCAAGGCATGACACAAG
AATCCTTTGATACCTCAGGTGCAGGTGCAGGGCGAGGGGCCGATCCCCGG
GTCCCTCCGTCGTCGCCCGCCACTCAGGTGTGCTTTGAGGAACCACAGCC
ATCAACATCGACGTCAGACCTCTTCCCCACAGCCTCGGCCTCTTCCACGG
AGCCTTCTCCTGCAGGGCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGT
GGTGGCATCTCCCCTAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGT
CTCCAGCTTTGCCTCAGCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGT
CGTTGGAACCCCAGGATCAGGAGGATTTGGAGCCCGTGAAGAAGAAAATG
AGAGGAGATGGGGACCTTGACCTGAACGGGCAGTTGTTGGTCGCACAACC
GCGTAGAAATGCCCAAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGA
AGCCAGACAAGATGGAGGAGACGCTGACATGCATCATCTGCCAGGACCTG
CTGCACGACTGCGTGAGTTTGCAGCCCTGCATGCACACGTTCTGCGCGGC
TTGCTACTCGGGCTGGATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCT
GTCCCGTGGAGCGGATCTGTAAAAACCACATCCTCAACAACCTCGTGGAA
GCATACCTCATCCAGCATCCAGACAAGAGTCGCAGTGAAGAAGATGTGCA
AAGTATGGATGCCAGGAATAAAATCACTCAAGACATGCTGCAGCCCAAAG
TCAGGCGGTCTTTTTCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAG
CTGTCAGACGTTGACAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGT
GTGCCGGCAGTGTCCTGAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACT
GCCCAGCACCCGAGGGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCA
CCCTCCACGTCCGTCAGCCTGACGACAGTCCAGGATTACGTGTGCCCTCT
GCAAGGAAGCCACGCCCTGTGCACCTGCTGCTTCCAGCCCATGCCCGACC
GGAGAGCGGAGCGCGAGCAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCG
GTCTGCCTGCAGCCTTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGG
CTGCTACGGCTGCCTGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGT
GTCTGGACGGCGTGCTGAACAACAACAGCTACGAGTCAGACATCCTGAAG
AATTACCTGGCAACCAGAGGTTTGACATGGAAAAACATGTTGACCGAGAG
CCTCGTGGCTCTCCAGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCA
CGGGAGACACCGTTCTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAG
CTGACCTATCAGTATCGGCAGAACATTCCTGCTTCCGAGTTGCCAGTGGC
CGTAACATCCCGTCCTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGG
TGAAAGCTCACCACGCCATGAAATTCAATCATATCTGTGAACAGACAAGG
TTCAAAAACTAAGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMERPEEGKQSPPPQPWGRL
LRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKS
GQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYL
YESLSEKQGMTQESFDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTST
SDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFA
SALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNA
QTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSG
WMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDA
RNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQC
PEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTVQDYVCPLQGSH
ALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGC
LAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVAL
QRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSR
PDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Silent change at R382 (AGA > AGG) and delta Ala470 (isoform 1 numbering)
isoform 3 = delta E135-K146 compared to isoform 1 NCBI ref
100042 R 1000 1001 1047 19007
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