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Synonyms
E3 ubiquitin-protein ligase CHFR isoform 1, RNF116, RNF196, checkpoint with forkhead and ring finger domains
Plasmid
pS-B-CHFR isoform 1 precursor
Parent Plasmid
pS-B
DU Number
DU29265
Genbank
NM_001161346.1 → NP_001154818.1
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCC
CTGGGGACGGCTCCTGCGTCTGGGCGCGGAGGAGGGCGAGCCGCACGTCC
TCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGCGACCTT
TCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGT
GGATGAAAAATCAGGTCAGGTGACACTGGAAGATACCAGCACCAGTGGAA
CAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGACATGCCCTTTACAG
ACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAA
CGTGGCATACCTCTATGAATCTTTAAGTGAAAAGCAAGGCATGACACAAG
AATCCTTTGAAGCTAACAAGGAAAATGTGTTCCATGGGACCAAAGATACC
TCAGGTGCAGGTGCAGGGCGAGGGGCCGATCCCCGGGTCCCTCCGTCGTC
GCCCGCCACTCAGGTGTGCTTTGAGGAACCACAGCCATCAACATCGACGT
CAGACCTCTTCCCCACAGCCTCGGCCTCTTCCACGGAGCCTTCTCCTGCA
GGGCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGTGGTGGCATCTCCCC
TAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGTCTCCAGCTTTGCCT
CAGCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGGAACCCCAG
GATCAGGAGGATTTGGAGCCCGTGAAGAAGAAAATGAGAGGAGATGGGGA
CCTTGACCTGAACGGGCAGTTGTTGGTCGCACAACCGCGTAGAAATGCCC
AAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGAAGCCAGACAAGATG
GAGGAGACGCTGACATGCATCATCTGCCAGGACCTGCTGCACGACTGCGT
GAGTTTGCAGCCCTGCATGCACACGTTCTGCGCGGCTTGCTACTCGGGCT
GGATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCTGTCCCGTGGAGCGG
ATCTGTAAAAACCACATCCTCAACAACCTCGTGGAAGCATACCTCATCCA
GCATCCAGACAAGAGTCGCAGTGAAGAAGATGTGCAAAGTATGGATGCCA
GGAATAAAATCACTCAAGACATGCTGCAGCCCAAAGTCAGGCGGTCTTTT
TCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCAGACGTTGA
CAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTC
CTGAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACTGCCCAGCACCCGAG
GGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCACCCTCCACGTCCGT
CAGCCTGACGACAGTCCAGGATTACGTGTGCCCTCTGCAAGGAAGCCACG
CCCTGTGCACCTGCTGCTTCCAGCCCATGCCCGACCGGAGAGCGGAGCGC
GAGCAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCGGTCTGCCTGCAGCC
TTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGGCTGCTACGGCTGCC
TGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGTGTCTGGACGGCGTG
CTGAACAACAACAGCTACGAGTCAGACATCCTGAAGAATTACCTGGCAAC
CAGAGGTTTGACATGGAAAAACATGTTGACCGAGAGCCTCGTGGCTCTCC
AGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCACGGGAGACACCGTT
CTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTGACCTATCAGTA
TCGGCAGAACATTCCTGCTTCCGAGTTGCCAGTGGCCGTAACATCCCGTC
CTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGGTGAAAGCTCACCAC
GCCATGAAATTCAATCATATCTGTGAACAGACAAGGTTCAAAAACTAAGC
GGCCGC
CTGGGGACGGCTCCTGCGTCTGGGCGCGGAGGAGGGCGAGCCGCACGTCC
TCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGCGACCTT
TCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGT
GGATGAAAAATCAGGTCAGGTGACACTGGAAGATACCAGCACCAGTGGAA
CAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGACATGCCCTTTACAG
ACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAA
CGTGGCATACCTCTATGAATCTTTAAGTGAAAAGCAAGGCATGACACAAG
AATCCTTTGAAGCTAACAAGGAAAATGTGTTCCATGGGACCAAAGATACC
TCAGGTGCAGGTGCAGGGCGAGGGGCCGATCCCCGGGTCCCTCCGTCGTC
GCCCGCCACTCAGGTGTGCTTTGAGGAACCACAGCCATCAACATCGACGT
CAGACCTCTTCCCCACAGCCTCGGCCTCTTCCACGGAGCCTTCTCCTGCA
GGGCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGTGGTGGCATCTCCCC
TAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGTCTCCAGCTTTGCCT
CAGCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGGAACCCCAG
GATCAGGAGGATTTGGAGCCCGTGAAGAAGAAAATGAGAGGAGATGGGGA
CCTTGACCTGAACGGGCAGTTGTTGGTCGCACAACCGCGTAGAAATGCCC
AAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGAAGCCAGACAAGATG
GAGGAGACGCTGACATGCATCATCTGCCAGGACCTGCTGCACGACTGCGT
GAGTTTGCAGCCCTGCATGCACACGTTCTGCGCGGCTTGCTACTCGGGCT
GGATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCTGTCCCGTGGAGCGG
ATCTGTAAAAACCACATCCTCAACAACCTCGTGGAAGCATACCTCATCCA
GCATCCAGACAAGAGTCGCAGTGAAGAAGATGTGCAAAGTATGGATGCCA
GGAATAAAATCACTCAAGACATGCTGCAGCCCAAAGTCAGGCGGTCTTTT
TCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCAGACGTTGA
CAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTC
CTGAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACTGCCCAGCACCCGAG
GGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCACCCTCCACGTCCGT
CAGCCTGACGACAGTCCAGGATTACGTGTGCCCTCTGCAAGGAAGCCACG
CCCTGTGCACCTGCTGCTTCCAGCCCATGCCCGACCGGAGAGCGGAGCGC
GAGCAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCGGTCTGCCTGCAGCC
TTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGGCTGCTACGGCTGCC
TGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGTGTCTGGACGGCGTG
CTGAACAACAACAGCTACGAGTCAGACATCCTGAAGAATTACCTGGCAAC
CAGAGGTTTGACATGGAAAAACATGTTGACCGAGAGCCTCGTGGCTCTCC
AGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCACGGGAGACACCGTT
CTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTGACCTATCAGTA
TCGGCAGAACATTCCTGCTTCCGAGTTGCCAGTGGCCGTAACATCCCGTC
CTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGGTGAAAGCTCACCAC
GCCATGAAATTCAATCATATCTGTGAACAGACAAGGTTCAAAAACTAAGC
GGCCGC
Amino Acid Sequence
View Sequence
MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSF
PSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTG
DVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFEANKENVFHGTKDTSG
AGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGR
ERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQ
EDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEE
TLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERIC
KNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSD
EEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGE
PGAPQALGDAPSTSVSLTTVQDYVCPLQGSHALCTCCFQPMPDRRAEREQ
DPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLN
NNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLC
YCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAM
KFNHICEQTRFKN*
PSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTG
DVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFEANKENVFHGTKDTSG
AGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGR
ERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQ
EDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEE
TLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERIC
KNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSD
EEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGE
PGAPQALGDAPSTSVSLTTVQDYVCPLQGSHALCTCCFQPMPDRRAEREQ
DPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLN
NNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLC
YCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAM
KFNHICEQTRFKN*
Vector Type
Bacterial
Antibiotic
Amp; Kan
100042 R 1000 1001 1047 19007
Download datasheet
Silent change at R382 (AGA > AGG) and delta Ala470 (isoform 1 numbering) compared to isoform 1 NCBI ref