This reagent and more can be found at our sister site MRC PPU Reagents & Services
Request Now
Synonyms
x
Plasmid
pSc-B-DTX3 isoform B
Parent Plasmid
pSC-B
DU Number
DU29258
Genbank
NM_001286245.1 → NP_001273174.1
Species
Human
Sequence of Insert
View Sequence
ggatccATGCCAATTCTAAGCTCTTCAGGATCAAAAATGGCAGCCTGTGG
AGGCACCTGCAAGAACAAAGTGACTGTGTCCAAGCCCGTGTGGGACTTCC
TGAGCAAAGAGACCCCAGCCCGGCTGGCCCGGCTTCGGGAGGAGCACCGT
GTGTCCATCCTCATAGATGGCGAGACTTCTGACATCTATGTTCTCCAGCT
TTCCCCACAGGGTCCTCCCCCGGCCCCTCCAAATGGGCTCTACCTAGCCC
GGAAGGCTCTCAAGGGGCTGCTAAAAGAGGCAGAGAAAGAGCTGAAGAAA
GCTCAGAGGCAGGGGGAGCTGATGGGCTGCCTGGCTCTGGGGGGTGGAGG
GGAGCACCCTGAGATGCACCGCGCAGGCCCACCCCCTCTCCGAGCAGCCC
CACTTCTGCCCCCAGGAGCTCGGGGGCTCCCCCCTCCTCCTCCCCCCCTG
CCCCCACCTCTTCCTCCTCGCCTTCGGGAGGAGGCAGAAGAGCAGGAGAG
CACCTGCCCCATCTGTCTGGGGGAGATCCAGAATGCCAAGACATTGGAGA
AGTGCCGGCATTCATTCTGCGAGGGCTGCATCACCCGGGCTCTGCAGGTG
AAAAAGGCCTGCCCCATGTGCGGCCGCTTCTATGGGCAGCTGGTGGGCAA
CCAGCCCCAGAATGGGCGGATGCTGGTCTCTAAGGACGCCACCCTCCTAC
TGCCCAGCTATGAGAAGTACGGCACCATTGTCATCCAGTACGTCTTCCCG
CCCGGTGTCCAGGGGGCTGAACACCCAAACCCAGGAGTTCGGTATCCTGG
CACCACACGGGTGGCCTACCTCCCGGACTGCCCTGAGGGCAACAAGGTGC
TGACCCTGTTCCGCAAGGCGTTTGACCAGCGTCTCACCTTCACTATCGGC
ACGTCCATGACCACAGGGAGACCGAATGTCATCACCTGGAACGACATCCA
CCACAAGACCAGCTGCACAGGGGGACCCCAGCTGTTTGGGTACCCAGACC
CCACCTACCTGACCCGGGTGCAAGAGGAGCTGAGAGCGAAGGGTATCACA
GATGACTGAgcggccgc
AGGCACCTGCAAGAACAAAGTGACTGTGTCCAAGCCCGTGTGGGACTTCC
TGAGCAAAGAGACCCCAGCCCGGCTGGCCCGGCTTCGGGAGGAGCACCGT
GTGTCCATCCTCATAGATGGCGAGACTTCTGACATCTATGTTCTCCAGCT
TTCCCCACAGGGTCCTCCCCCGGCCCCTCCAAATGGGCTCTACCTAGCCC
GGAAGGCTCTCAAGGGGCTGCTAAAAGAGGCAGAGAAAGAGCTGAAGAAA
GCTCAGAGGCAGGGGGAGCTGATGGGCTGCCTGGCTCTGGGGGGTGGAGG
GGAGCACCCTGAGATGCACCGCGCAGGCCCACCCCCTCTCCGAGCAGCCC
CACTTCTGCCCCCAGGAGCTCGGGGGCTCCCCCCTCCTCCTCCCCCCCTG
CCCCCACCTCTTCCTCCTCGCCTTCGGGAGGAGGCAGAAGAGCAGGAGAG
CACCTGCCCCATCTGTCTGGGGGAGATCCAGAATGCCAAGACATTGGAGA
AGTGCCGGCATTCATTCTGCGAGGGCTGCATCACCCGGGCTCTGCAGGTG
AAAAAGGCCTGCCCCATGTGCGGCCGCTTCTATGGGCAGCTGGTGGGCAA
CCAGCCCCAGAATGGGCGGATGCTGGTCTCTAAGGACGCCACCCTCCTAC
TGCCCAGCTATGAGAAGTACGGCACCATTGTCATCCAGTACGTCTTCCCG
CCCGGTGTCCAGGGGGCTGAACACCCAAACCCAGGAGTTCGGTATCCTGG
CACCACACGGGTGGCCTACCTCCCGGACTGCCCTGAGGGCAACAAGGTGC
TGACCCTGTTCCGCAAGGCGTTTGACCAGCGTCTCACCTTCACTATCGGC
ACGTCCATGACCACAGGGAGACCGAATGTCATCACCTGGAACGACATCCA
CCACAAGACCAGCTGCACAGGGGGACCCCAGCTGTTTGGGTACCCAGACC
CCACCTACCTGACCCGGGTGCAAGAGGAGCTGAGAGCGAAGGGTATCACA
GATGACTGAgcggccgc
Amino Acid Sequence
View Sequence
MPILSSSGSKMAACGGTCKNKVTVSKPVWDFLSKETPARLARLREEHRVS
ILIDGETSDIYVLQLSPQGPPPAPPNGLYLARKALKGLLKEAEKELKKAQ
RQGELMGCLALGGGGEHPEMHRAGPPPLRAAPLLPPGARGLPPPPPPLPP
PLPPRLREEAEEQESTCPICLGEIQNAKTLEKCRHSFCEGCITRALQVKK
ACPMCGRFYGQLVGNQPQNGRMLVSKDATLLLPSYEKYGTIVIQYVFPPG
VQGAEHPNPGVRYPGTTRVAYLPDCPEGNKVLTLFRKAFDQRLTFTIGTS
MTTGRPNVITWNDIHHKTSCTGGPQLFGYPDPTYLTRVQEELRAKGITDD
*
ILIDGETSDIYVLQLSPQGPPPAPPNGLYLARKALKGLLKEAEKELKKAQ
RQGELMGCLALGGGGEHPEMHRAGPPPLRAAPLLPPGARGLPPPPPPLPP
PLPPRLREEAEEQESTCPICLGEIQNAKTLEKCRHSFCEGCITRALQVKK
ACPMCGRFYGQLVGNQPQNGRMLVSKDATLLLPSYEKYGTIVIQYVFPPG
VQGAEHPNPGVRYPGTTRVAYLPDCPEGNKVLTLFRKAFDQRLTFTIGTS
MTTGRPNVITWNDIHHKTSCTGGPQLFGYPDPTYLTRVQEELRAKGITDD
*
Vector Type
Other
Antibiotic
Amp; Kan
100042 R 1000 1001 1047 19007
Download datasheet
amplified product flanked BamHI-Not1 but internal Not1 discovered site at nt615. Can be excised for subcloning with BamHI-Sal1 (using 3' Sal1 site from pSc-B backbone)