CHFR | Ubiquitylation Resource

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Synonyms

E3 ubiquitin-protein ligase CHFR isoform 1, RNF116, RNF196, checkpoint with forkhead and ring finger domains

Plasmid

pS-B-CHFR isoform 3

Parent Plasmid

pS-B

DU Number

DU29234

Genbank

NM_001161346.1 → NP_001154818.1

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCC
CTGGGGACGGCTCCTGCGTCTGGGCGCGGAGGAGGGCGAGCCGCACGTCC
TCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGCGACCTT
TCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGT
GGATGAAAAATCAGGTCAGGTGACACTGGAAGATACCAGCACCAGTGGAA
CAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGACATGCCCTTTACAG
ACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAA
CGTGGCATACCTCTATGAATCTTTAAGTGAAAAGCAAGGCATGACACAAG
AATCCTTTGATACCTCAGGTGCAGGTGCAGGGCGAGGGGCCGATCCCCGG
GTCCCTCCGTCGTCGCCCGCCACTCAGGTGTGCTTTGAGGAACCACAGCC
ATCAACATCGACGTCAGACCTCTTCCCCACAGCCTCGGCCTCTTCCACGG
AGCCTTCTCCTGCAGGGCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGT
GGTGGCATCTCCCCTAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGT
CTCCAGCTTTGCCTCAGCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGT
CGTTGGAACCCCAGGATCAGGAGGATTTGGAGCCCGTGAAGAAGAAAATG
AGAGGAGATGGGGACCTTGACCTGAACGGGCAGTTGTTGGTCGCACAACC
GCGTAGAAATGCCCAAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGA
AGCCAGACAAGATGGAGGAGACGCTGACATGCATCATCTGCCAGGACCTG
CTGCACGACTGCGTGAGTTTGCAGCCCTGCATGCACACGTTCTGCGCGGC
TTGCTACTCGGGCTGGATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCT
GTCCCGTGGAGCGGATCTGTAAAAACCACATCCTCAACAACCTCGTGGAA
GCATACCTCATCCAGCATCCAGACAAGAGTCGCAGTGAAGAAGATGTGCA
AAGTATGGATGCCAGGAATAAAATCACTCAAGACATGCTGCAGCCCAAAG
TCAGGCGGTCTTTTTCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAG
CTGTCAGACGTTGACAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGT
GTGCCGGCAGTGTCCTGAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACT
GCCCAGCACCCGAGGGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCA
CCCTCCACGTCCGTCAGCCTGACGACAGTCCAGGATTACGTGTGCCCTCT
GCAAGGAAGCCACGCCCTGTGCACCTGCTGCTTCCAGCCCATGCCCGACC
GGAGAGCGGAGCGCGAGCAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCG
GTCTGCCTGCAGCCTTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGG
CTGCTACGGCTGCCTGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGT
GTCTGGACGGCGTGCTGAACAACAACAGCTACGAGTCAGACATCCTGAAG
AATTACCTGGCAACCAGAGGTTTGACATGGAAAAACATGTTGACCGAGAG
CCTCGTGGCTCTCCAGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCA
CGGGAGACACCGTTCTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAG
CTGACCTATCAGTATCGGCAGAACATTCCTGCTTCCGAGTTGCCAGTGGC
CGTAACATCCCGTCCTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGG
TGAAAGCTCACCACGCCATGAAATTCAATCATATCTGTGAACAGACAAGG
TTCAAAAACTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSF
PSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTG
DVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFDTSGAGAGRGADPRVP
PSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGG
ISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRG
DGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLH
DCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAY
LIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELS
DVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPS
TSVSLTTVQDYVCPLQGSHALCTCCFQPMPDRRAEREQDPRVAPQQCAVC
LQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNY
LATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELT
YQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFK
N*

Vector Type

Bacterial

Antibiotic

Amp; Kan

Comments

Silent change at R382 (AGA > AGG) and delta Ala470 (isoform 1 numbering)
isoform 3 = delta E135-K146 compared to isoform 1 NCBI ref

100042 R 1000 1001 1047 19007

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