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Plasmid
pcDNA5D FRT/TO- NMNAT2 C-ThrLess-C-FLAG
Parent Plasmid
pcDNA5D FRT/TO-C-FLAG
Expressed
NMNAT2-FLAG Thr to Ala (10 C-terminal)
DU Number
DU29083
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
CTCGAGGCCGCCATGACCGAGACCACCAAGACCCACGTTATCTTGCTCGC
CTGCGGCAGCTTCAATCCCATCACCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAACTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACACCTGGCAGACGACCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGGCTGGCTGCAT
CCTCTCCAATGTCAACGCTCCTTCCATGGCTCCTGTGATCGGACAGCCAC
AAAACGAGGCCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCGCCAAG
CCCGCTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCGCCTTTGTAGATGAGAATG
CCAATCTGGGCGCGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACGCTGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAGCCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC
CTGCGGCAGCTTCAATCCCATCACCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAACTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACACCTGGCAGACGACCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGGCTGGCTGCAT
CCTCTCCAATGTCAACGCTCCTTCCATGGCTCCTGTGATCGGACAGCCAC
AAAACGAGGCCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCGCCAAG
CCCGCTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCGCCTTTGTAGATGAGAATG
CCAATCTGGGCGCGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACGCTGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAGCCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC
Amino Acid Sequence
View Sequence
MTETTKTHVILLACGSFNPITKGHIQMFERARDYLHKTGRFIVIGGIVSP
VHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWECYQDTWQTTCSVLE
HHRDLMKRVAGCILSNVNAPSMAPVIGQPQNEAPQPIYQNSNVAAKPAAA
KILGKVGESLSRICCVRPPVERFAFVDENANLGAVMRYEEIELRILLLCG
SDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADADRIMNHSSILRKY
KNNIMVVKDDINHPMSVVSSAKSRLALQHGDGHVVDYLSQPVIDYILKSQ
LYINASGAAADYKDDDDK*
VHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWECYQDTWQTTCSVLE
HHRDLMKRVAGCILSNVNAPSMAPVIGQPQNEAPQPIYQNSNVAAKPAAA
KILGKVGESLSRICCVRPPVERFAFVDENANLGAVMRYEEIELRILLLCG
SDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADADRIMNHSSILRKY
KNNIMVVKDDINHPMSVVSSAKSRLALQHGDGHVVDYLSQPVIDYILKSQ
LYINASGAAADYKDDDDK*
Vector Type
Mammalian
Antibiotic
Amp
Comments
C-terminal 10 Thr residues mutated to Ala. Parental 41573
100042 R 1000 1001 1047 19007
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