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Plasmid
pFBDMb 6His TEVG mouse USP1 delta M1-V69, E222-Q419 and L607-V735 C90A/mouse WDR48
Parent Plasmid
pFBDMb
Expressed
6His TEVG mouse USP1 delta M1-V69, E222-Q419 and L607-V735 C90A
DU Number
DU58262
Genbank
NM_001301414.1
Tag
6His
Species
Mouse
Sequence of Insert
View Sequence
GGATCCGAAACCATGCATCACCATCACCATCACGAAAACCTGTATTTTCAGGGCAGCTGCGAGAAGAGAGAGAACTTGTTACCATTTGTGGGATTGAATAACCTTGGCAACACTGCCTATCTGAATAGTATTCTTCAGGTGTTATACTTTTGTCCTGGCTTTAAGACTGGAGTGAAGCACTTATTTAATATTATTTCAAGGAAGAAAGAAGCTCTGAAGGATGACTCTAATCAGAAGGATAAGGGAAGCTGCAAAGAAGAGTCTTTGGCAAGTTATGAGCTTATATGTAGTTTACAGTCCTTAATAATTTCAGTTGAACAGCTTCAGGCTAGTTTTCTCTTAAATCCAGAGAAATATACAGATGAACTTGCTACACAGCCAAGGCGACTGCTGAACACACTCAGGGAACTCAACCCTATGTATGAAGGATTTCTACAGCATGATGCACAGGAAGTGCTACAGTGTATTCTGGGAAACATTCAAGAAACATGCCAACTCCTAAAAAAAGAAATTGGCTTTGAGCTAGTAGAGAAACTGTTTCAAGGTCAGCTCGTGTTAAGGACTCGGTGTTTGGAATGTGAAAGCTTAACAGAAAGAAGAGAAGATTTCCAGGATATTAGTGTTCCAGTGCAAGAAGATGAGCTTTCCAAGGTCGAGGAGAGCTCTGAAATTTCTCCAGAGCCAAAAACAGAAATGAAGACCTTGAGATGGGCAATTTCACAGTTTGCTTCAGTGGAGAGAATTGTAGGAGAGGATAAATACTTCTGTGAAAATTGCCATCATTATACGGAAGCAGAGCGAAGTCTCCTGTTTGATAAAATGCCTGAAGTTATCACTATTCATCTGAAGTGCTTTGCTGCTAGTGGCTTGGAGTTTGACTGTTACGGTGGTGGACTTTCCAAGATCAACACTCCTTTATTGACACCTCTTAAACTGTCACTAGAAGAATGGAGCACAAAGCCGACGAATGACAGCTATGGATTATTTGCCGTTGTGATGCACAGTGGCATTACTATTAGTAGTGGGCACTATACCGCATCTGTTAAAGTCACTGACCTTAACAGTCTAGAGGTGCAAAGCTTAAAGGAGTACGAGGGGAAGTGGTTGCTCTTTGACGATTCTGAGGTAAAAGTGACAGAAGAGAAAGACTTTCTGAATTCTCTCTCCCCTTCTACATCCCCTACGTCTACTCCCTACTTGCTATTCTATAAAAAATTATAGCGGCCGC
Amino Acid Sequence
View Sequence
MHHHHHHENLYFQGSCEKRENLLPFVGLNNLGNTAYLNSILQVLYFCPGFKTGVKHLFNIISRKKEALKDDSNQKDKGSCKEESLASYELICSLQSLIIS
VEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGFLQHDAQEVLQCILGNIQETCQLLKKEIGFELVEKLFQGQLVLRTRCLECESLTERREDFQ
DISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINT
PLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLEVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYK
KL*
VEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGFLQHDAQEVLQCILGNIQETCQLLKKEIGFELVEKLFQGQLVLRTRCLECESLTERREDFQ
DISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINT
PLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLEVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYK
KL*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
Mutagenesis of DU58257 (RT14180/14181)
100042 R 1000 1001 1047 19007
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