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Plasmid
pFBDMb 6His TEVG mouse USP1 delta E222-Q419 and L607-V735/mouse WDR48
Parent Plasmid
pFBDMb
Expressed
6His TEVG mouse USP1 delta E222-Q419 and L607-V735
DU Number
DU58112
Genbank
NM_001301414.1
Tag
6His
Species
Mouse
Sequence of Insert
View Sequence
GGATCCATGCATCACCATCACCATCACGAAAACCTGTATTTTCAGGGCATGCCTGGCGTCATACCTAGTGAAAGTAATGGGCTTTCAAGAGGCAGTCCATCCAAGAAAAACAGACTGTCTCTGAAGTTTTTTCAGAAAAAGGAAACTAAAAGAGCCTTAGACTTTACTGATTCTCAAGAAAATGAAGAAAAAACTTCTGAATATAGAGGATCTGAGATTGACCAAGTTGTTCCTGCTGCACAGTCCTCCCCAGTAAGCTGCGAGAAGAGAGAGAACTTGTTACCATTTGTGGGATTGAATAACCTTGGCAACACTTGTTATCTGAATAGTATTCTTCAGGTGTTATACTTTTGTCCTGGCTTTAAGACTGGAGTGAAGCACTTATTTAATATTATTTCAAGGAAGAAAGAAGCTCTGAAGGATGACTCTAATCAGAAGGATAAGGGAAGCTGCAAAGAAGAGTCTTTGGCAAGTTATGAGCTTATATGTAGTTTACAGTCCTTAATAATTTCAGTTGAACAGCTTCAGGCTAGTTTTCTCTTAAATCCAGAGAAATATACAGATGAACTTGCTACACAGCCAAGGCGACTGCTGAACACACTCAGGGAACTCAACCCTATGTATGAAGGATTTCTACAGCATGATGCACAGGAAGTGCTACAGTGTATTCTGGGAAACATTCAAGAAACATGCCAACTCCTAAAAAAAGAAATTGGCTTTGAGCTAGTAGAGAAACTGTTTCAAGGTCAGCTCGTGTTAAGGACTCGGTGTTTGGAATGTGAAAGCTTAACAGAAAGAAGAGAAGATTTCCAGGATATTAGTGTTCCAGTGCAAGAAGATGAGCTTTCCAAGGTCGAGGAGAGCTCTGAAATTTCTCCAGAGCCAAAAACAGAAATGAAGACCTTGAGATGGGCAATTTCACAGTTTGCTTCAGTGGAGAGAATTGTAGGAGAGGATAAATACTTCTGTGAAAATTGCCATCATTATACGGAAGCAGAGCGAAGTCTCCTGTTTGATAAAATGCCTGAAGTTATCACTATTCATCTGAAGTGCTTTGCTGCTAGTGGCTTGGAGTTTGACTGTTACGGTGGTGGACTTTCCAAGATCAACACTCCTTTATTGACACCTCTTAAACTGTCACTAGAAGAATGGAGCACAAAGCCGACGAATGACAGCTATGGATTATTTGCCGTTGTGATGCACAGTGGCATTACTATTAGTAGTGGGCACTATACCGCATCTGTTAAAGTCACTGACCTTAACAGTCTAGAGGTGCAAAGCTTAAAGGAGTACGAGGGGAAGTGGTTGCTCTTTGACGATTCTGAGGTAAAAGTGACAGAAGAGAAAGACTTTCTGAATTCTCTCTCCCCTTCTACATCCCCTACGTCTACTCCCTACTTGCTATTCTATAAAAAATTATAGCGGCCGC
Amino Acid Sequence
View Sequence
MHHHHHHENLYFQGMPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKTSEYRGSEIDQVVPAAQSSPVSCEKRENLLPFVGLNNL
GNTCYLNSILQVLYFCPGFKTGVKHLFNIISRKKEALKDDSNQKDKGSCKEESLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLR
ELNPMYEGFLQHDAQEVLQCILGNIQETCQLLKKEIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTL
RWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHS
GITISSGHYTASVKVTDLNSLEVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL*
GNTCYLNSILQVLYFCPGFKTGVKHLFNIISRKKEALKDDSNQKDKGSCKEESLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLR
ELNPMYEGFLQHDAQEVLQCILGNIQETCQLLKKEIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTL
RWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHS
GITISSGHYTASVKVTDLNSLEVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
mouse WDR48 XhoI/KpnI into DU58087
100042 R 1000 1001 1047 19007
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