MYCBP2 | Ubiquitylation Resource

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Plasmid

pcDNA5-FRT/TO-MYCBP2 Leu3348-Phe4640 C4561S-myc

Parent Plasmid

pcDNA5-FRT/TO

Expressed

MYCBP2 Leu3348-Phe4640 C4561S-myc

DU Number

DU28910

Tag

Myc

Species

Human

Sequence of Insert

View Sequence

GGATCCatgCTTCCTGTGAAAATGCCTTGTCTCTACCTGCAGACATTAGC
TAGGCATCATCATGAAAATTTTGTGGGCTATCAAGATGACAATCTATTCC
AGGATGAAATGAGATATCTACGTTCAACATCTGTACCTGCCCCGTATATA
TCAGTAACTCCTGATGCAAGTCCTAATGTATTTGAAGAGCCAGAGAGCAA
TATGAAGTCTATGCCACCAAGTTTAGAAACCAGTCCCATAACTGATACTG
ATCTTGCAAAGAGAACTGTCTTCCAAAGATCATACTCAGTTGTTGCTTCC
GAATATGATAAACAACACTCCATTTTACCTGCACGAGTTAAAGCTATTCC
TAGAAGAAGAGTTAACAGTGGAGACACTGAAGTTGGTTCTTCCCTTTTGA
GACATCCGTCTCCTGAGCTTTCTCGGCTAATCTCAGCCCACAGCTCTCTT
TCTAAAGGAGAACGAAATTTCCAGTGGCCAGTTTTAGCTTTTGTTATACA
ACATCATGATCTAGAAGGTCTTGAAATAGCAATGAAACAGGCCCTAAGGA
AATCTGCTTGTCGAGTTTTTGCTATGGAGGCTTTCAACTGGCTTCTGTGT
AATGTCATCCAAACCACTTCTCTCCATGATATTCTGTGGCATTTTGTGGC
ATCACTGACTCCTGCACCAGTGGAACCAGAGGAAGAAGAGGATGAAGAAA
ATAAAACAAGCAAAGAAAATTCAGAACAAGAGAAAGATACAAGAGTATGT
GAACATCCACTCTCAGACATAGTGATTGCCGGGGAAGCTGCTCATCCTTT
ACCACACACCTTTCACCGCTTGCTGCAGACCATCTCAGACCTTATGATGT
CTCTCCCCAGCGGCAGTTCATTACAGCAAATGGCCCTGAGGTGCTGGAGT
CTCAAATTCAAGCAATCTGATCACCAGTTCCTTCATCAGAGCAACGTCTT
TCATCACATTAACAATATTTTGTCAAAGTCAGATGATGGCGATAGTGAAG
AGAGTTTTAGCATCAGTATACAGTCTGGCTTTGAAGCTATGAGTCAGGAA
TTATGCATAGTAATGTGCTTAAAGGACTTAACCAGCATTGTTGACATAAA
AACTTCAAGCCGACCTGCCATGATTGGCAGTTTGACAGACGGCTCCACAG
AAACCTTTTGGGAATCAGGAGATGAAGATAAAAACAAAACTAAGAACATC
ACCATCAACTGTGTAAAAGGAATCAATGCCCGCTATGTGTCTGTTCACGT
GGACAATTCCCGAGATCTTGGGAATAAAGTTACCTCAATGACCTTCTTAA
CTGGCAAAGCAGTAGAAGATTTGTGCAGAATAAAGCAGGTTGATCTGGAT
TCCAGGCACATTGGCTGGGTAACAAGTGAACTTCCAGGAGGGGATAATCA
CATCATAAAAATTGAATTAAAAGGCCCAGAAAATACACTGAGAGTTCGAC
AAGTCAAAGTCCTGGGCTGGAAAGATGGTGAAAGCACAAAAATAGCTGGC
CAGATTTCAGCCAGTGTGGCCCAGCAGAGGAACTGTGAAGCTGAGACTCT
GCGAGTATTCAGACTGATTACGTCTCAAGTATTTGGAAAGCTCATCTCTG
GAGATGCTGAACCTACACCAGAACAAGAGGAAAAAGCACTATTGTCATCA
CCTGAAGGAGAAGAAAAAGTATACAATGCAACATCAGATGCTGACCTGAA
AGAACATATGGTTGGAATCATATTCAGCAGGAGTAAGCTGACTAACTTAC
AAAAACAGGTGTGTGCTCATATTGTCCAAGCTATTCGCATGGAAGCTACC
AGAGTCCGTGAAGAATGGGAACATGCTATATCAAGCAAAGAAAATGCCAA
TTCTCAGCCAAATGATGAAGATGCCTCCTCTGATGCCTACTGCTTTGAGC
TGCTCTCTATGGTTTTAGCACTGAGTGGCTCTAACGTTGGCCGGCAATAT
CTGGCTCAACAGCTAACCCTGCTTCAGGATCTCTTCTCGCTGCTTCACAC
AGCCTCTCCTAGAGTCCAGAGACAGGTAACCTCTTTACTAAGAAGAGTTT
TGCCTGAAGTAACCCCTAGTCGTCTGGCCAGCATCATAGGAGTGAAATCC
CTCCCCCCAGCAGATATCAGTGATATCATTCACTCAACAGAGAAAGGAGA
CTGGAATAAGCTGGGTATCTTGGACATGTTTCTAGGATGCATTGCCAAAG
CACTCACTGTACAGCTAAAAGCCAAAGGAACCACCATCACTGGAACAGCT
GGTACCACTGTGGGCAAAGGAGTTACAACAGTTACTCTTCCGATGATTTT
CAATTCCAGTTATCTCCGACGAGGTGAAAGTCATTGGTGGATGAAGGGCT
CAACCCCTACCCAGATCTCAGAGATCATCATTAAACTTATCAAGGATATG
GCAGCAGGTCATCTGTCAGAAGCTTGGTCCCGAGTGACAAAAAATGCTAT
TGCAGAAACCATCATTGCCTTGACCAAGATGGAAGAAGAATTTAGGTCTC
CAGTGAGATGTATTGCAACAACTAGACTCTGGCTTGCTCTCGCATCCCTA
TGTGTTCTTGATCAGGACCACGTAGATCGTCTCTCCTCGGGGAGATGGAT
GGGAAAGGATGGACAACAAAAACAAATGCCTATGTGTGATAACCATGATG
ATGGTGAAACTGCAGCAATCATTTTATGCAATGTCTGTGGAAATTTATGT
ACAGACTGTGACAGATTCCTTCACCTTCATCGAAGAACCAAAACTCATCA
AAGACAGGTCTTCAAAGAAGAAGAAGAAGCTATAAAGGTTGACCTTCATG
AAGGTTGTGGTAGAACCAAATTGTTCTGGTTGATGGCACTGGCAGATTCT
AAAACAATGAAGGCAATGGTGGAATTCCGAGAACACACAGGCAAACCCAC
CACGAGTAGCTCAGAAGCATGTCGCTTCTGTGGTTCCAGGAGTGGAACAG
AGTTATCTGCTGTTGGCAGTGTTTGTTCTGATGCAGATTGCCAGGAATAC
GCTAAGATAGCCTGTAGTAAGACGCATCCTTGTGGCCATCCATGCGGGGG
TGTTAAAAACGAAGAGCACTGTCTGCCCTGTCTACACGGCTGTGACAAAA
GTGCCACAAGCCTGAAGCAAGACGCCGATGACATGTGCATGATATGTTTC
ACCGAAGCGCTCTCGGCAGCACCAGCCATTCAGCTGGATTGTAGTCACAT
ATTCCACTTACAGTGCTGTCGGCGAGTATTAGAAAATCGATGGCTTGGCC
CAAGGATAACATTTGGATTTATATCTTGTCCCATTTGCAAGAACAAAATT
AATCACATAGTACTAAAAGACCTACTTGATCCAATAAAAGAACTCTATGA
GGATGTCAGAAGAAAAGCCTTAATGAGATTGGAATATGAAGGTCTGCATA
AGAGTGAAGCTATCACAACTCCTGGTGTGAGGTTTTATAATGACCCAGCT
GGCTATGCAATGAATAGATATGCATATTATGTGTGCTACAAATGCAGAAA
GGCATATTTTGGTGGTGAAGCTCGCTGCGATGCTGAGGCTGGACGGGGAG
ATGATTATGATCCCAGAGAGCTCATTTGTGGTGCCTGTTCTGATGTTTCC
AGGGCTCAGATGTGTCCCAAACATGGCACAGACTTTTTGGAATATAAATC
TCGCTACTGCTGTTCAGTGGCTGTTTTTTTCTGTTTTGGAACAACACATT
TTTGTAATGCTTGTCATGATGATTTTCAAAGAATGACTAGCATTCCTAAG
GAAGAACTACCACACTGTCCTGCAGGTCCCAAAGGCAAGCAGTTAGAAGG
AACTGAATGTCCACTCCATGTTGTTCATCCACCCACTGGGGAAGAGTTTG
CTCTGGGATGTGGAGTGTGCAGAAATGCCCACACTTTTGAACAAAAATTA
ATCTCAGAAGAAGACTTGTAGGCGGCCGC

Amino Acid Sequence

View Sequence

MLPVKMPCLYLQTLARHHHENFVGYQDDNLFQDEMRYLRSTSVPAPYISV
TPDASPNVFEEPESNMKSMPPSLETSPITDTDLAKRTVFQRSYSVVASEY
DKQHSILPARVKAIPRRRVNSGDTEVGSSLLRHPSPELSRLISAHSSLSK
GERNFQWPVLAFVIQHHDLEGLEIAMKQALRKSACRVFAMEAFNWLLCNV
IQTTSLHDILWHFVASLTPAPVEPEEEEDEENKTSKENSEQEKDTRVCEH
PLSDIVIAGEAAHPLPHTFHRLLQTISDLMMSLPSGSSLQQMALRCWSLK
FKQSDHQFLHQSNVFHHINNILSKSDDGDSEESFSISIQSGFEAMSQELC
IVMCLKDLTSIVDIKTSSRPAMIGSLTDGSTETFWESGDEDKNKTKNITI
NCVKGINARYVSVHVDNSRDLGNKVTSMTFLTGKAVEDLCRIKQVDLDSR
HIGWVTSELPGGDNHIIKIELKGPENTLRVRQVKVLGWKDGESTKIAGQI
SASVAQQRNCEAETLRVFRLITSQVFGKLISGDAEPTPEQEEKALLSSPE
GEEKVYNATSDADLKEHMVGIIFSRSKLTNLQKQVCAHIVQAIRMEATRV
REEWEHAISSKENANSQPNDEDASSDAYCFELLSMVLALSGSNVGRQYLA
QQLTLLQDLFSLLHTASPRVQRQVTSLLRRVLPEVTPSRLASIIGVKSLP
PADISDIIHSTEKGDWNKLGILDMFLGCIAKALTVQLKAKGTTITGTAGT
TVGKGVTTVTLPMIFNSSYLRRGESHWWMKGSTPTQISEIIIKLIKDMAA
GHLSEAWSRVTKNAIAETIIALTKMEEEFRSPVRCIATTRLWLALASLCV
LDQDHVDRLSSGRWMGKDGQQKQMPMCDNHDDGETAAIILCNVCGNLCTD
CDRFLHLHRRTKTHQRQVFKEEEEAIKVDLHEGCGRTKLFWLMALADSKT
MKAMVEFREHTGKPTTSSSEACRFCGSRSGTELSAVGSVCSDADCQEYAK
IACSKTHPCGHPCGGVKNEEHCLPCLHGCDKSATSLKQDADDMCMICFTE
ALSAAPAIQLDCSHIFHLQCCRRVLENRWLGPRITFGFISCPICKNKINH
IVLKDLLDPIKELYEDVRRKALMRLEYEGLHKSEAITTPGVRFYNDPAGY
AMNRYAYYVCYKCRKAYFGGEARCDAEAGRGDDYDPRELICGACSDVSRA
QMCPKHGTDFLEYKSRYCCSVAVFFCFGTTHFCNACHDDFQRMTSIPKEE
LPHCPAGPKGKQLEGTECPLHVVHPPTGEEFALGCGVCRNAHTFEQKLIS
EEDL*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Parental 41459

100042 R 1000 1001 1047 19007

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