MYCBP2 | Ubiquitylation Resource

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Plasmid

pcDNA5-FRT/TO-MYCBP2 Leu3348-Phe4640 C4520S-myc

Parent Plasmid

pcDNA5-FRT/TO

DU Number

DU28859

Tag

Myc

Species

Human

Sequence of Insert

View Sequence

ggatccCTTCCTGTGAAAATGCCTTGTCTCTACCTGCAGACATTAGCTAG
GCATCATCATGAAAATTTTGTGGGCTATCAAGATGACAATCTATTCCAGG
ATGAAATGAGATATCTACGTTCAACATCTGTACCTGCCCCGTATATATCA
GTAACTCCTGATGCAAGTCCTAATGTATTTGAAGAGCCAGAGAGCAATAT
GAAGTCTATGCCACCAAGTTTAGAAACCAGTCCCATAACTGATACTGATC
TTGCAAAGAGAACTGTCTTCCAAAGATCATACTCAGTTGTTGCTTCCGAA
TATGATAAACAACACTCCATTTTACCTGCACGAGTTAAAGCTATTCCTAG
AAGAAGAGTTAACAGTGGAGACACTGAAGTTGGTTCTTCCCTTTTGAGAC
ATCCGTCTCCTGAGCTTTCTCGGCTAATCTCAGCCCACAGCTCTCTTTCT
AAAGGAGAACGAAATTTCCAGTGGCCAGTTTTAGCTTTTGTTATACAACA
TCATGATCTAGAAGGTCTTGAAATAGCAATGAAACAGGCCCTAAGGAAAT
CTGCTTGTCGAGTTTTTGCTATGGAGGCTTTCAACTGGCTTCTGTGTAAT
GTCATCCAAACCACTTCTCTCCATGATATTCTGTGGCATTTTGTGGCATC
ACTGACTCCTGCACCAGTGGAACCAGAGGAAGAAGAGGATGAAGAAAATA
AAACAAGCAAAGAAAATTCAGAACAAGAGAAAGATACAAGAGTATGTGAA
CATCCACTCTCAGACATAGTGATTGCCGGGGAAGCTGCTCATCCTTTACC
ACACACCTTTCACCGCTTGCTGCAGACCATCTCAGACCTTATGATGTCTC
TCCCCAGCGGCAGTTCATTACAGCAAATGGCCCTGAGGTGCTGGAGTCTC
AAATTCAAGCAATCTGATCACCAGTTCCTTCATCAGAGCAACGTCTTTCA
TCACATTAACAATATTTTGTCAAAGTCAGATGATGGCGATAGTGAAGAGA
GTTTTAGCATCAGTATACAGTCTGGCTTTGAAGCTATGAGTCAGGAATTA
TGCATAGTAATGTGCTTAAAGGACTTAACCAGCATTGTTGACATAAAAAC
TTCAAGCCGACCTGCCATGATTGGCAGTTTGACAGACGGCTCCACAGAAA
CCTTTTGGGAATCAGGAGATGAAGATAAAAACAAAACTAAGAACATCACC
ATCAACTGTGTAAAAGGAATCAATGCCCGCTATGTGTCTGTTCACGTGGA
CAATTCCCGAGATCTTGGGAATAAAGTTACCTCAATGACCTTCTTAACTG
GCAAAGCAGTAGAAGATTTGTGCAGAATAAAGCAGGTTGATCTGGATTCC
AGGCACATTGGCTGGGTAACAAGTGAACTTCCAGGAGGGGATAATCACAT
CATAAAAATTGAATTAAAAGGCCCAGAAAATACACTGAGAGTTCGACAAG
TCAAAGTCCTGGGCTGGAAAGATGGTGAAAGCACAAAAATAGCTGGCCAG
ATTTCAGCCAGTGTGGCCCAGCAGAGGAACTGTGAAGCTGAGACTCTGCG
AGTATTCAGACTGATTACGTCTCAAGTATTTGGAAAGCTCATCTCTGGAG
ATGCTGAACCTACACCAGAACAAGAGGAAAAAGCACTATTGTCATCACCT
GAAGGAGAAGAAAAAGTATACAATGCAACATCAGATGCTGACCTGAAAGA
ACATATGGTTGGAATCATATTCAGCAGGAGTAAGCTGACTAACTTACAAA
AACAGGTGTGTGCTCATATTGTCCAAGCTATTCGCATGGAAGCTACCAGA
GTCCGTGAAGAATGGGAACATGCTATATCAAGCAAAGAAAATGCCAATTC
TCAGCCAAATGATGAAGATGCCTCCTCTGATGCCTACTGCTTTGAGCTGC
TCTCTATGGTTTTAGCACTGAGTGGCTCTAACGTTGGCCGGCAATATCTG
GCTCAACAGCTAACCCTGCTTCAGGATCTCTTCTCGCTGCTTCACACAGC
CTCTCCTAGAGTCCAGAGACAGGTAACCTCTTTACTAAGAAGAGTTTTGC
CTGAAGTAACCCCTAGTCGTCTGGCCAGCATCATAGGAGTGAAATCCCTC
CCCCCAGCAGATATCAGTGATATCATTCACTCAACAGAGAAAGGAGACTG
GAATAAGCTGGGTATCTTGGACATGTTTCTAGGATGCATTGCCAAAGCAC
TCACTGTACAGCTAAAAGCCAAAGGAACCACCATCACTGGAACAGCTGGT
ACCACTGTGGGCAAAGGAGTTACAACAGTTACTCTTCCGATGATTTTCAA
TTCCAGTTATCTCCGACGAGGTGAAAGTCATTGGTGGATGAAGGGCTCAA
CCCCTACCCAGATCTCAGAGATCATCATTAAACTTATCAAGGATATGGCA
GCAGGTCATCTGTCAGAAGCTTGGTCCCGAGTGACAAAAAATGCTATTGC
AGAAACCATCATTGCCTTGACCAAGATGGAAGAAGAATTTAGGTCTCCAG
TGAGATGTATTGCAACAACTAGACTCTGGCTTGCTCTCGCATCCCTATGT
GTTCTTGATCAGGACCACGTAGATCGTCTCTCCTCGGGGAGATGGATGGG
AAAGGATGGACAACAAAAACAAATGCCTATGTGTGATAACCATGATGATG
GTGAAACTGCAGCAATCATTTTATGCAATGTCTGTGGAAATTTATGTACA
GACTGTGACAGATTCCTTCACCTTCATCGAAGAACCAAAACTCATCAAAG
ACAGGTCTTCAAAGAAGAAGAAGAAGCTATAAAGGTTGACCTTCATGAAG
GTTGTGGTAGAACCAAATTGTTCTGGTTGATGGCACTGGCAGATTCTAAA
ACAATGAAGGCAATGGTGGAATTCCGAGAACACACAGGCAAACCCACCAC
GAGTAGCTCAGAAGCATGTCGCTTCTGTGGTTCCAGGAGTGGAACAGAGT
TATCTGCTGTTGGCAGTGTTTGTTCTGATGCAGATTGCCAGGAATACGCT
AAGATAGCCTGTAGTAAGACGCATCCTTGTGGCCATCCATGCGGGGGTGT
TAAAAACGAAGAGCACTGTCTGCCCTGTCTACACGGCTGTGACAAAAGTG
CCACAAGCCTGAAGCAAGACGCCGATGACATGTGCATGATATGTTTCACC
GAAGCGCTCTCGGCAGCACCAGCCATTCAGCTGGATTGTAGTCACATATT
CCACTTACAGTGCTGTCGGCGAGTATTAGAAAATCGATGGCTTGGCCCAA
GGATAACATTTGGATTTATATCTTGTCCCATTTGCAAGAACAAAATTAAT
CACATAGTACTAAAAGACCTACTTGATCCAATAAAAGAACTCTATGAGGA
TGTCAGAAGAAAAGCCTTAATGAGATTGGAATATGAAGGTCTGCATAAGA
GTGAAGCTATCACAACTCCTGGTGTGAGGTTTTATAATGACCCAGCTGGC
TATGCAATGAATAGATATGCATATTATGTGTGCTACAAATGCAGAAAGGC
ATATTTTGGTGGTGAAGCTCGCTcCGATGCTGAGGCTGGACGGGGAGATG
ATTATGATCCCAGAGAGCTCATTTGTGGTGCCTGTTCTGATGTTTCCAGG
GCTCAGATGTGTCCCAAACATGGCACAGACTTTTTGGAATATAAATGTCG
CTACTGCTGTTCAGTGGCTGTTTTTTTCTGTTTTGGAACAACACATTTTT
GTAATGCTTGTCATGATGATTTTCAAAGAATGACTAGCATTCCTAAGGAA
GAACTACCACACTGTCCTGCAGGTCCCAAAGGCAAGCAGTTAGAAGGAAC
TGAATGTCCACTCCATGTTGTTCATCCACCCACTGGGGAAGAGTTTGCTC
TGGGATGTGGAGTGTGCAGAAATGCCCACACTTTTGAACAAAAATTAATC
TCAGAAGAAGACTTGTAGGCGGCCGC

Amino Acid Sequence

View Sequence

LPVKMPCLYLQTLARHHHENFVGYQDDNLFQDEMRYLRSTSVPAPYISVT
PDASPNVFEEPESNMKSMPPSLETSPITDTDLAKRTVFQRSYSVVASEYD
KQHSILPARVKAIPRRRVNSGDTEVGSSLLRHPSPELSRLISAHSSLSKG
ERNFQWPVLAFVIQHHDLEGLEIAMKQALRKSACRVFAMEAFNWLLCNVI
QTTSLHDILWHFVASLTPAPVEPEEEEDEENKTSKENSEQEKDTRVCEHP
LSDIVIAGEAAHPLPHTFHRLLQTISDLMMSLPSGSSLQQMALRCWSLKF
KQSDHQFLHQSNVFHHINNILSKSDDGDSEESFSISIQSGFEAMSQELCI
VMCLKDLTSIVDIKTSSRPAMIGSLTDGSTETFWESGDEDKNKTKNITIN
CVKGINARYVSVHVDNSRDLGNKVTSMTFLTGKAVEDLCRIKQVDLDSRH
IGWVTSELPGGDNHIIKIELKGPENTLRVRQVKVLGWKDGESTKIAGQIS
ASVAQQRNCEAETLRVFRLITSQVFGKLISGDAEPTPEQEEKALLSSPEG
EEKVYNATSDADLKEHMVGIIFSRSKLTNLQKQVCAHIVQAIRMEATRVR
EEWEHAISSKENANSQPNDEDASSDAYCFELLSMVLALSGSNVGRQYLAQ
QLTLLQDLFSLLHTASPRVQRQVTSLLRRVLPEVTPSRLASIIGVKSLPP
ADISDIIHSTEKGDWNKLGILDMFLGCIAKALTVQLKAKGTTITGTAGTT
VGKGVTTVTLPMIFNSSYLRRGESHWWMKGSTPTQISEIIIKLIKDMAAG
HLSEAWSRVTKNAIAETIIALTKMEEEFRSPVRCIATTRLWLALASLCVL
DQDHVDRLSSGRWMGKDGQQKQMPMCDNHDDGETAAIILCNVCGNLCTDC
DRFLHLHRRTKTHQRQVFKEEEEAIKVDLHEGCGRTKLFWLMALADSKTM
KAMVEFREHTGKPTTSSSEACRFCGSRSGTELSAVGSVCSDADCQEYAKI
ACSKTHPCGHPCGGVKNEEHCLPCLHGCDKSATSLKQDADDMCMICFTEA
LSAAPAIQLDCSHIFHLQCCRRVLENRWLGPRITFGFISCPICKNKINHI
VLKDLLDPIKELYEDVRRKALMRLEYEGLHKSEAITTPGVRFYNDPAGYA
MNRYAYYVCYKCRKAYFGGEARSDAEAGRGDDYDPRELICGACSDVSRAQ
MCPKHGTDFLEYKCRYCCSVAVFFCFGTTHFCNACHDDFQRMTSIPKEEL
PHCPAGPKGKQLEGTECPLHVVHPPTGEEFALGCGVCRNAHTFEQKLISE
EDL*

Vector Type

Mammalian

Antibiotic

Amp

Comments

ORF has no iniating methionine. Parental 41459

100042 R 1000 1001 1047 19007

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