This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Plasmid
pYES2 His YPL191c
Parent Plasmid
pYES
Expressed
Gal promoter- 6His-YPL191c
DU Number
DU55483
Genbank
NM_001184005.1.
Sequence of Insert
View Sequence
GGATCCATGCATCATCACCATCACCATGATTTAAGTTTCACGACTAAAAGTGTCAAAATTAATGGTCAAAACCATCGAATATTACTGCAAAACGAAAACGGACCTTGTGCCTTGTTAGCACTTGCAAATATTCTAATACTTTCACCAGATCATACACGTTTTTCCAATGAGTTAATAAGGTTGGTAAATAAGGGAAGTCAAATATCTTTAAAAGAACTTATTGAAGTCTTAGCAGATATCGCTTTACAAGTTACAGATAAACCTAGTACTGATATAAGTGAGTTACTAAGTTTGTTACCTAGATTACATGAAGGTTTAAATATTAACCCTGAGTTTAATGGGTCTTTTGAAAATACTAAAGAGATGTCAATATTTCGATTGTTTAACGTTGATGTTGTCCATGGCTGGGTTATCAACAGTTTCATAAATGAAAACATTGATGAGAAACTATCTCATTATTCTTATGAATCTGCGCAGAGAATCTTAACACAAGCAGCTGATATAAATTGTGGAATTTCCCAAGATGAAAACTCGGACGAAGTTTTACGTGATGCCATGCATTTGGGTCTGTTTTTGAACGAATCACCGACTCAGTTGACCGCCTTTGGACTCCTACGATTGAGAGAAAAGCTGCTACACAACAAATTCTCCATTTTATTCAGAAATGACCATTTTTCTACATTGTTCAAATACGAAGATCGATTATACACATTGGTTACGGACTTTGGTTATAAAAATTGTAAAGATATTGTATGGCAATCCCTAGATTCGGTGGACGGCTCCTGCGATGCTTTTTTTGCCGGTAACTTTAGTGCAGCAGAGGTAAATGGACAGCAATTGTCAACTGACATTGAACGTGATTTTGGAACTGGAAATTTGCTACTTGAAGAAATTCAGCAAATTGAAAACGATAAAGAACTAGCCAAACAGTTACAGGAACAAGAACAAGAACGTGTTACGAAATTTGAAGCAAAAAGGAAAATCCATTCTCACAAAAAAAACTCAGAAATACACGCACCAGTCAAAAAAGACAAGTTTAAGCGAAGAAGCAGCCTTCTCAATGCAAAAGCTTCTGAAAAAGAGAAAAGTGAGTGCGTTGTTATGTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MHHHHHHDLSFTTKSVKINGQNHRILLQNENGPCALLALANILILSPDHTRFSNELIRLVNKGSQISLKELIEVLADIALQVTDKPSTDISELLSLLPRLHEGLNINPEFNGSFENTKEMSIFRLFNVDVVHGWVINSFINENIDEKLSHYSYESAQRILTQAADINCGISQDENSDEVLRDAMHLGLFLNESPTQLTAFGLLRLREKLLHNKFSILFRNDHFSTLFKYEDRLYTLVTDFGYKNCKDIVWQSLDSVDGSCDAFFAGNFSAAEVNGQQLSTDIERDFGTGNLLLEEIQQIENDKELAKQLQEQEQERVTKFEAKRKIHSHKKNSEIHAPVKKDKFKRRSSLLNAKASEKEKSECVVM*
Vector Type
Yeast
100042 R 1000 1001 1047 19007
Download datasheet
Could you clone the YPL191c gene under the Gal promoter, with a 6His tag at the N-terminal of YPL191c. The selection marker can be any auxotrophy (leu, ura, his, trp or ade), or antibiotic except for hygromycin.
Thank you
Marjorie