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Plasmid
pYES2 His YGL082W
Parent Plasmid
pYES2
Expressed
Gal promoter-6His-YGL082w
DU Number
DU55456
Genbank
NM_001180947.3
Tag
6His
Sequence of Insert
View Sequence
GAATTCATGCATCATCACCATCACCATGATGTTACATTTCTAACCAAAAATGTTCAAATAAATGGCACTCAGTTTAAAATATTACTTCAAAATGGGCAAGGTGAATGCGCGCTAATTGCACTAGCTAACGTATTATTGATATCACCTGCCCATGCTCGCTATGCCCAAGAAATTTCCAGACTAGTGAGAGGCAAGGAAACTGTTACTTTAAACGAACTTGTGCAAACTCTAGCCGACATGGGCGTTCAAAATCCGAATGGTACAGATGTGGACAAACAACAGCTACTCCAAATCCTACCACAATTATATAGCGGATTGAACATCAACCCAGAATTTAACGGATCTTTTGAGGACGGCGTAGAAATGTCGATTTTCAGGCTCTACAATGTCGGCATCGTTCATGGATGGATAATAGATGGGGACAATGATCCGAACTCTTACGAACATGTGTCCAAATATTCTTATATGGGCGCGCAAAAAGTTTTGGTACAGTCGTATGAGATCCAAAAGAATAATGCCCAATTTGAAAACAGCGAGCAAATCCAATCTGATGCACCTTACTTAAAATCGTTTCTGGCAAGATCTGCCACACAATTGACTGAATATGGTCTAACTCATCTCAGAGAAATTTTAGTAGAAAGATCGTATGCCGTGCTTTTTAGAAATGATCACTTTTGCACATTATATAAAAACAATGGCGAATTGTTTACTTTGGTCACGGATCCTACTTACAGAAATCGCAAGGACATAAACTGGCAGTCGCTTAAATCCGTGAATGGATCGCAGGATTCTTACTACACGGGCAACTTCATTCCCACAAGTCTGGAAAGAACAGAAACTACTGCTACCGGGCAAAATGAGTCCTACATTTCTAACCCATTCAGTGACCAGAATACAGGGCATGTAACTAGCAACCAAGTTAATAGTGGAGCAAGCGGTGTTCAACAAATCGAAGATGATGAGGAACTAGCTAGGAGGTTACAGGAACAAGAAGATATGCGTGCGGCAAATAACATGCAAAATGGTTATGCTAATAACGGTAGAAATCATCAAAGGGAAAGGTTTGAAAGACCTGAAAAAAATTCAAAGAAAAACAAGTTCCTTCCCTTTAATGGGAGTAATAAAGAAAAGAAAAGAGACAAGTTGAAAAAAAACTGTGTAATTATGTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MHHHHHHDVTFLTKNVQINGTQFKILLQNGQGECALIALANVLLISPAHARYAQEISRLVRGKETVTLNELVQTLADMGVQNPNGTDVDKQQLLQILPQLYSGLNINPEFNGSFEDGVEMSIFRLYNVGIVHGWIIDGDNDPNSYEHVSKYSYMGAQKVLVQSYEIQKNNAQFENSEQIQSDAPYLKSFLARSATQLTEYGLTHLREILVERSYAVLFRNDHFCTLYKNNGELFTLVTDPTYRNRKDINWQSLKSVNGSQDSYYTGNFIPTSLERTETTATGQNESYISNPFSDQNTGHVTSNQVNSGASGVQQIEDDEELARRLQEQEDMRAANNMQNGYANNGRNHQRERFERPEKNSKKNKFLPFNGSNKEKKRDKLKKNCVIM*
Vector Type
Yeast
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Could you clone the YGL082w gene under the GAL promoter, with a 6His tag at the N terminal. You can use any selection marker you wish except Hygromycin.
Thanks,
Marjorie