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HECTD1

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Plasmid
pGEX C3 HALO thr-HECTD1 Met2149-2610 (END)
Parent Plasmid
pGEX C3 HALO thr
Expressed
GST-HALO-HECTD1 Met2149-2610 (END)
DU Number
DU28626
Genbank
Q9ULT8-1 (No genbank)
Tag
GST
HALO
Species
Human
Sequence of Insert
ggatccATGCAAATACATGCAGATCGGAAATCAGTTCTTGAGGTTGAATT
TTTAGGAGAAGAAGGAACTGGCTTGGGACCCACATTAGAGTTTTATGCTC
TGGTGGCAGCAGAATTCCAGAGAACTGACTTGGGAGCTTGGCTTTGTGAT
GATAATTTTCCAGATGATGAATCTCGTCACGTTGATCTTGGAGGTGGATT
GAAACCTCCTGGATATTATGTGCAGAGGTCATGTGGACTGTTCACAGCAC
CATTTCCACAGGATAGTGATGAGCTTGAAAGGATCACGAAACTGTTTCAT
TTCCTTGGAATTTTCTTGGCCAAATGCATTCAAGACAATAGACTTGTGGA
CTTACCTATTTCTAAACCTTTTTTTAAACTTATGTGTATGGGTGACATTA
AAAGCAATATGAGTAAACTGATTTATGAGTCACGAGGTGATAGAGACTTA
CACTGTACTGAAAGTCAGTCTGAAGCTTCTACAGAAGAAGGTCATGATTC
ACTCTCGGTAGGAAGCTTTGAAGAGGATTCAAAATCAGAATTTATTCTTG
ATCCCCCTAAACCAAAACCCCCAGCTTGGTTTAATGGAATTTTGACTTGG
GAAGACTTTGAATTAGTAAACCCACACAGAGCCAGATTTTTAAAAGAAAT
TAAAGACCTTGCTATCAAGAGGCGCCAAATTTTAAGCAACAAAGGTCTTT
CTGAAGATGAGAAGAACACAAAATTACAGGAACTAGTGCTGAAGAATCCA
TCAGGTTCTGGGCCTCCACTTAGCATAGAGGATTTAGGTTTAAATTTCCA
GTTTTGCCCTTCCTCAAGAATATATGGTTTTACAGCTGTGGATCTCAAGC
CAAGTGGTGAAGATGAGATGATAACAATGGATAATGCAGAAGAATATGTG
GATTTGATGTTTGACTTTTGTATGCATACGGGTATTCAGAAACAAATGGA
AGCCTTTAGAGATGGGTTTAATAAAGTTTTTCCAATGGAGAAATTAAGTT
CCTTCAGCCATGAAGAAGTCCAAATGATTCTTTGTGGAAACCAGTCACCA
TCCTGGGCAGCAGAGGATATTATCAATTACACTGAACCTAAGCTGGGTTA
TACACGTGACAGCCCTGGTTTCCTGAGGTTTGTGAGGGTTTTATGTGGCA
TGTCTTCTGATGAAAGGAAAGCATTCTTGCAGTTTACCACTGGTTGTTCA
ACTCTACCCCCAGGTGGACTGGCTAACCTGCATCCCAGGCTCACGGTTGT
ACGCAAGGTTGATGCTACTGATGCAAGCTATCCATCAGTCAATACATGTG
TGCATTACCTTAAGTTGCCTGAATATTCTTCCGAGGAGATCATGAGAGAG
CGCCTGCTAGCTGCTACAATGGAGAAAGGCTTTCATCTCAATTGAgcggc
cgc
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMQIHADRKSVLEVE
FLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGG
LKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLV
DLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHD
SLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKE
IKDLAIKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNF
QFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQM
EAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLG
YTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTV
VRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Grow at or below 30ºC
100042 R 1000 1001 1047 19007
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