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BRAP

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Plasmid
pGEX6P HALO Thr-BRAP
Parent Plasmid
pGEX 3C HALO Thr
Expressed
GST-HALO-BRAP
DU Number
DU28603
Genbank
NP_006759.3 (uni: Q7Z569-1)
Tag
GST
HALO
Species
Human
Sequence of Insert
ggatccATGAGTGTGTCACTGGTTGTTATCCGATTGGAGCTCGCGGAACA
CTCGCCTGTCCCCGCCGGCTTCGGCTTCAGCGCCGCGGCCGGGGAAATGT
CTGATGAGGAGATAAAAAAGACGACACTAGCCTCAGCTGTAGCCTGTTTA
GAAGGCAAGTCACCAGGAGAGAAAGTAGCGATTATCCATCAGCATCTCGG
CCGTCGAGAAATGACAGATGTGATCATTGAGACCATGAAGTCCAACCCAG
ATGAACTAAAAACTACAGTGGAAGAAAGGAAGTCTTCAGAAGCCTCCCCC
ACTGCGCAAAGAAGTAAAGATCACAGTAAGGAATGCATAAACGCTGCCCC
AGATTCTCCGTCCAAACAGCTTCCAGACCAGATTTCATTCTTCAGTGGAA
ATCCATCAGTTGAAATAGTTCATGGTATTATGCACCTATATAAGACAAAT
AAGATGACCTCCTTAAAAGAAGATGTGCGGCGCAGTGCCATGCTGTGTAT
TCTCACAGTCCCTGCTGCAATGACCAGTCATGACCTTATGAAGTTTGTTG
CCCCATTTAACGAAGTAATTGAACAAATGAAAATTATCAGAGACTCTACT
CCCAACCAATATATGGTGCTGATAAAGTTTCGTGCACAGGCTGATGCGGA
TAGTTTTTATATGACATGCAATGGCCGCCAGTTCAACTCAATAGAAGATG
ACGTTTGCCAGCTAGTGTATGTGGAAAGAGCTGAAGTGCTCAAATCTGAA
GATGGCGCCAGCCTCCCAGTGATGGACCTGACTGAACTCCCCAAGTGCAC
GGTGTGTCTGGAGCGCATGGACGAGTCTGTGAATGGCATCCTCACAACGT
TATGTAACCACAGCTTCCACAGCCAGTGTCTACAGCGCTGGGACGATACC
ACGTGTCCTGTTTGCCGGTACTGTCAAACGCCCGAGCCAGTAGAAGAAAA
TAAGTGTTTTGAGTGTGGTGTTCAGGAAAATCTTTGGATTTGTTTAATAT
GCGGCCACATAGGATGTGGACGGTATGTCAGTCGACATGCTTATAAGCAC
TTTGAGGAAACGCAGCACACGTATGCCATGCAGCTTACCAACCATCGAGT
CTGGGACTATGCTGGAGATAACTATGTTCATCGACTGGTTGCAAGTAAAA
CAGATGGAAAAATAGTACAGTATGAATGTGAGGGGGATACTTGCCAGGAA
GAGAAAATAGATGCCTTACAGTTAGAGTATTCATATTTACTAACAAGCCA
GCTGGAATCTCAGCGAATCTACTGGGAAAACAAGATAGTTCGGATAGAGA
AGGACACAGCAGAGGAAATTAACAACATGAAGACCAAGTTTAAAGAAACA
ATTGAGAAGTGTGATAATCTAGAGCACAAACTAAATGATCTCCTAAAAGA
AAAGCAGTCTGTGGAAAGAAAGTGCACTCAGCTAAACACAAAAGTGGCCA
AACTCACCAACGAGCTCAAAGAGGAGCAGGAAATGAACAAGTGTTTGCGA
GCCAACCAAGTCCTCCTGCAGAACAAGCTAAAAGAGGAGGAGAGGGTGCT
GAAGGAGACCTGTGACCAAAAAGATCTGCAGATCACCGAGATCCAGGAGC
AGCTGCGTGACGTCATGTTCTACCTGGAGACACAGCAGAAGATCAACCAT
CTGCCTGCCGAGACCCGGCAGGAAATCCAGGAGGGACAGATCAACATCGC
CATGGCCTCGGCCTCGAGCCCTGCCTCTTCGGGGGGCAGTGGGAAGTTGC
CCTCCAGGAAGGGCCGCAGCAAGAGGGGCAAGTGAgcggccgc
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMSVSLVVIRLELAE
HSPVPAGFGFSAAAGEMSDEEIKKTTLASAVACLEGKSPGEKVAIIHQHL
GRREMTDVIIETMKSNPDELKTTVEERKSSEASPTAQRSKDHSKECINAA
PDSPSKQLPDQISFFSGNPSVEIVHGIMHLYKTNKMTSLKEDVRRSAMLC
ILTVPAAMTSHDLMKFVAPFNEVIEQMKIIRDSTPNQYMVLIKFRAQADA
DSFYMTCNGRQFNSIEDDVCQLVYVERAEVLKSEDGASLPVMDLTELPKC
TVCLERMDESVNGILTTLCNHSFHSQCLQRWDDTTCPVCRYCQTPEPVEE
NKCFECGVQENLWICLICGHIGCGRYVSRHAYKHFEETQHTYAMQLTNHR
VWDYAGDNYVHRLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTS
QLESQRIYWENKIVRIEKDTAEEINNMKTKFKETIEKCDNLEHKLNDLLK
EKQSVERKCTQLNTKVAKLTNELKEEQEMNKCLRANQVLLQNKLKEEERV
LKETCDQKDLQITEIQEQLRDVMFYLETQQKINHLPAETRQEIQEGQINI
AMASASSPASSGGSGKLPSRKGRSKRGK*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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