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Plasmid
pGEX6P1 YPL191C 1-270 C28A
Parent Plasmid
pGEX6P-1
Expressed
GST-YPL191C 1-270 C28A
DU Number
DU55338
Tag
GST
Sequence of Insert
View Sequence
GGATCCCCCGGGCTGCAGCGACCAATGTGGAATTCGCCCTTGGATCTGATGGATTTAAGTTTCACGACTAAAAGTGTCAAAATTAATGGTCAAAACCATCGAATATTACTGCAAAACGAAAACGGACCTGCTGCCTTGTTAGCACTTGCAAATATTCTAATACTTTCACCAGATCATACACGTTTTTCCAATGAGTTAATAAGGTTGGTAAATAAGGGAAGTCAAATATCTTTAAAAGAACTTATTGAAGTCTTAGCAGATATCGCTTTACAAGTTACAGATAAACCTAGTACTGATATAAGTGAGTTACTAAGTTTGTTACCTAGATTACATGAAGGTTTAAATATTAACCCTGAGTTTAATGGGTCTTTTGAAAATACTAAAGAGATGTCAATATTTCGATTGTTTAACGTTGATGTTGTCCATGGCTGGGTTATCAACAGTTTCATAAATGAAAACATTGATGAGAAACTATCTCATTATTCTTATGAATCTGCGCAGAGAATCTTAACACAAGCAGCTGATATAAATTGTGGAATTTCCCAAGATGAAAACTCGGACGAAGTTTTACGTGATGCCATGCATTTGGGTCTGTTTTTGAACGAATCACCGACTCAGTTGACCGCCTTTGGACTCCTACGATTGAGAGAAAAGCTGCTACACAACAAATTCTCCATTTTATTCAGAAATGACCATTTTTCTACATTGTTCAAATACGAAGATCGATTATACACATTGGTTACGGACTTTGGTTATAAAAATTGTAAAGATATTGTATGGCAATCCCTAGATTCGGTGGACGGCTCCTGCGATGCTTTTTTTGCCGGTAACTTTAGTGCAGCAGAGGTAAATGGACAGTAACAATTGTCAACTGACATTGAACGTGATTTTGGAACTGGAAATTTGCTACTTGAAGAAATTCAGCAAATTGAAAACGATAAAGAACTAGCCAAACAGTTACAGGAACAAGAACAAGAACGTGTTACGAAATTTGAAGCAAAAAGGAAAATCCATTCTCACAAAAAAAACTCAGAAATACACGCACCAGTCAAAAAAGACAAGTTTAAGCGAAGAAGCAGCCTTCTCAATGCAAAAGCTTCTGAAAAAGAGAAAAGTGAGTGCGTTGTTATGTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGLQRPMWNSPLDLMDLSFTTKSVKINGQNHRILLQNENGPAALLALANILILSPDHTRFSNELIRLVNKGSQISLKELIEVLADIALQVTDKPSTDISELLSLLPRLHEGLNINPEFNGSFENTKEMSIFRLFNVDVVHGWVINSFINENIDEKLSHYSYESAQRILTQAADINCGISQDENSDEVLRDAMHLGLFLNESPTQLTAFGLLRLREKLLHNKFSILFRNDHFSTLFKYEDRLYTLVTDFGYKNCKDIVWQSLDSVDGSCDAFFAGNFSAAEVNGQ*
Vector Type
Bacterial
Comments
please mutate Cys 28 to Ala in 55257
100042 R 1000 1001 1047 19007
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