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Synonyms
CG13745; Dmel\CG13745; fanci
Plasmid
pFBDM 6His GST 3C Drosophila Fancd2/6His 3C Drosophila FancI
Parent Plasmid
pFBDM
Expressed
6His 3C Drosophila FancI
DU Number
DU53448
Genbank
NM_136585.3 GI:665399661 with changes - see notes
Tag
6His
Species
Drosophila Melanogaster
Sequence of Insert
View Sequence
CCCGGGATGCACCACCATCACCATCACTTGGAGGTCTTGTTTCAAGGACCTATGCGGAGTCTGGGTGAGAAGGGGCGCTTTGATGAGCTGGAACTGCTCATTCAAAACACTCCAATAGAAAAGTTAAAGGAAACCCTTAAATCCACAATGACACGCAGCGAGGGCATAACGTATTGGAACTACCTTCTCCGTGGCTTCAATCCGGAGAGCAAGGATGCGCTAGCCAAGCGATTCGAATGCGTCCGCAGCTTCATGGACGGACTGAGCAGCACGGAACTGAGCTACAAACAGACTTTCGATCTGATCACGCGCCTGTCGCAGGACCTGGCCACCTTTCCGTCCGAGCAGCTGGCCTGGATCGTGGAGCACTGCATGGACGGGATCCGACAAGGAGACGCCAAGTGCGTCGGCTGGAAGGATTTGTTGCCGGACGCGCTGTCGCTGCTTTTGGCCAGACCGCGGTTTCTTCTGAACGGCATATCCATAGATGGAATGGAGTTCAGGGACACTACCGTAAGGAGCATTGCCACCATGCAATGGCCAGCCTCAATCCTCACGCCAATTGCCGACATGTTCAAGACCATGAATCTTAGCAGTGGCGAAATTGTGACAGTACTCAACAAATTCTCTGGCGCCCTGCAGGAGCTATCGCCCATGGAACTCCCAGCGCTGTGCTTCCAGCTCTTTTCGATGTGCAGCACTGCTTCCCAGTTGATCATTACGTTGCTGGCCCTGGAGAAGTATTTCCATCGCAACTACTACAAGCGGCTCTTCTCAGACATGTGTAGCAACTCAACGAACTTGGATAGCATTGACTTGTTCTCGGACAAGGAACTAAGGGAGGCCGAAGAGACTATCCTGCATCACTTGAACTACTGTACTATGTACAAACTTACTGAAAAACATTTGGCCATAATGCTGAGAAACTTTCTGCACATGCCCGACGTGATCCTAACTCCGTTTATGCTTAGCGCCATTATCTCGATGACCAGCATTAATCGCGATCCAGAGTCCGCCAGAATATCTCATTCCATTTTGCTACCTTTTTTGCGCAATGTGATCAAAAACAACGAGGAAGAGCGAACGTTAGCCGACTACTCCGTGTGGTATCGGGATACATTGCAACGCAAGCAAGTCGATTTGCAGCAGGTCCTCACGGTGCTCATTGATCAGAACAAAGACGGCAAGGATGTGATAACTCCTGGGCTGGTTCATCTCGTATTTTACTTGCTGAAATCGCAATCGCCCAAGATGCACACATTGGCCATCACATTTCTAACCAAGTTCATTCGCAAGCGATTCATTTTTGGACAAGGCATCATAAAGCTTATTTCAGAGTGGATGATAGTATACCAGGAACAAAATCAGTTCTCGGAATGCCTCACACTTCTAAGTGTGGCTGATACGTATACGGTCAGCGAGTGCATGAAACCAATTCAATCTGTTTTGGAGCACATGCAATGGCTGTCTGGTGAGCAGTCCATGCGCATGATGAACTTTATTCTACCTTTGCTAAAGATCTCTAATCGCGTCCGTGATGCGCTAATTGATGTACTTTGCAAAGCAATGAGCTCGAGTGGAATTCAAAAACGGCGAATGGCGATTTATGGGTTCTGCATGATCCTCAAACAACTAAACAACAGCAATGCTGTGCGCCAGACCTCCAGCGCCACCAGTTTCTGCACTCAACACAGTATCTCTGGCTACTCCATAATGACCCAGAACACACTGGGAAGTCGTAGTAATCCTCAAAGAAATTTCGACATGCTCACGTTGGAAATTATTGGAATGTTGCGAAACTGCTTGCAACAACAATTCGATATACGGTGCTCCCTGTATGAAAATCTTCAGAGAGCAGTGGAGCTCAATGCTAAACTAGTGCCACATGTGCTGCAAGTCATTGACTGGCACTTTCGCAGCTTCTTCGACACCCCCTCTGCAGAGGATGCTGGTGACGATCTAGACACAGTATTCCGCATTCGCTACGATCAATTGGTTAGCTCTAGCGATGATCAACACATGGAGATTCAGCTTAAAGACAACCTGGGCAAGTTGATTCAGTTCGTGGCCAACTGCTTGGCTGTTTTCGATCGAGCTCCATCGGGCTACGACACTCGTGAGATGAATCGGCTCATGAGTTTCTGTGTGGATCGTATGGTGGCAAACAGATTACCCCTGGAAGAGATAACGCCGCCAGCCACACATCTAAAATGTGCCCTCGTTCTGCAGCAACTGAATATCATTGAAGGAATTATTTGCCACTTATTGCTCAAATCCAAGCCACAAAACAATGCTGTTTGTCAAATATTGCCTCTTTTTAATCAGCATTGTAAGCTTTTGGAGAGTCTTAAGGCATTATCGGATGCATCCAAGAAAGCCCATAAACAGTTGAAGCAAACCGCCAAGAATAATAGCACATCAACGTGCAATTTGACACTGAACGGCGTCCCGGTGAAGACCATGTGTACACAGCCTGATAATATTTGGGACTTGGCCATATTGGATAAGCTGCTCCATCTACTTCTTGATGACGTCGTAGCCTTTGCTGCTCCAGAAAAAACAGTGCTCTTGCGCTCCAACGAGCCCCTCGTCCGGTATGTTTTATCCGTAACGGCCAGCAGAGTGGAGTCTATACGCCTGGAGCCCGATTATAAGCAGTTGGCCTACAGTAAACGCACCTTTAAGCAATTGACGGACATTACAAAGGTCATTTACGAGCGCTGCATTCAACGCTTGCCGGAGCTGTGGAAAAATTTCGACATGCAGAGCGCCGCCCTAGCCACTCAGTGTTTTTCTGAATGCCTAAAAACAGCTCATGAAACGTACTCAAAGAAGTTCGAAGATTTTGTCAAGTCATTTGATTTGGCATCTATAAAGGGAAGCAGAAACATGGAAGTGGTTTACACAATTCAGGATGTTTTAGACGACTTTATGACGGAGTACGGCACTGATGATTCGATTTGTAAAAATGAGTTCGTTTCCAAATTGCCTGTTTACCTCTTGGAGTCGCTTGAAATTCTTTTGGATCACATTGACTACCAGGATCGTGCTGCCACCGAATCGTACACTTGGCTGCTAAATTTTTGCAGCAAAAACGAGATTCTCAACAGTGAAATGGGCCTGGTGCACAGAATGCTATTCGTTCAGCGTCAAAAGACCCACTTAGGTCCCTTCTTCGACAGTGTTGCTCGGCAATTGGGTCACGTACTGGGCGTACAAAATGAAGACGCTCCCTCAGAATCTGTGGAGCTAACTCTAAAGTCCATAAGTACAGTGACCATCGAGAGTTGCCTACAGCATCTGTATGCTGCCATCCAAAAGCAGCTGAACGATGTGGACTACTTTGTGACGAAAGCCAACAATCTAAACTACAAATGCCAAGTGGTTCCCGAAATTGACCAGATATATTGGCGGGGCAACCTGGATTCCATGGATCGTTCCATTTGCACACAAATAATCCACATAAGTCGCACTTTGCTGGTGCTCACAAACGTTTGTATTCCTCTAGGTTCTTTGATGGACGGTCTTATGAAGTTGTTGATTCAGCACTACACTTGTTTGAAAAGCCTGACCAAGCACTACATATCGAGCTACACATCAGATACGTCCATTGAGAACATTAAAAGCACCAAATTTGAACTTCTGCTACGTGCAGTTGGTAAACAACTTCCGGCGAATATTTACGAGCTCATCACGTACATAGAAGCTAATACCCTGGATGAGGAAGCGCAGCAGCATACAAAAAAGCGGAAGGTCCAGGCAGAACGTGCTAAAGTTCTGAGAGAGACTCGGCTAATTCCCAAGGTTATAATGGTTATCGAGGACTTCAATAAGCACATTATTCTCCTGTCCAAAAAGACCAAAAGCAAGAATAGACTTACGGACTACCTCCACTTGGGAACCATGCGCGACTTCGATATCAAGTCGACGGATTTGAGGGCAGCTATAGAGCGTAGTATCTCGGATGGCCGCAACATTTCTACCGAAGACAGTAATGCTGAGGATCGGGATGAGGATGAGGATGAAAATGAGAATGTAGAGGAAGACGATGACGCACAGTCCACCACGTATCCAGAGGACTTGGAGGAGAAGGAAGATGAATGCTTAGTCATAGAGAAGAAGGAAGAAAGGCGCAGGAGGCGTGCACCTAGCAGTGAAGCAGAGGATCCACAACCCAAACGAAAAAGAGGCCGCAAAGCTGCAGAACAGCTGGAAGCTGAAGATGAAGAGCAAGTCGAGGAAGAGCAGCCTGAAACGACTTTAAAATTAAGGCGAAACAAAAAAAGTGGAGCCAAAAACCAAAAGGAGTGTGGCCATTAAGGTACC
Amino Acid Sequence
View Sequence
MHHHHHHLEVLFQGPMRSLGEKGRFDELELLIQNTPIEKLKETLKSTMTRSEGITYWNYLLRGFNPESKDALAKRFECVRSFMDGLSSTELSYKQTFDLI
TRLSQDLATFPSEQLAWIVEHCMDGIRQGDAKCVGWKDLLPDALSLLLARPRFLLNGISIDGMEFRDTTVRSIATMQWPASILTPIADMFKTMNLSSGEI
VTVLNKFSGALQELSPMELPALCFQLFSMCSTASQLIITLLALEKYFHRNYYKRLFSDMCSNSTNLDSIDLFSDKELREAEETILHHLNYCTMYKLTEKH
LAIMLRNFLHMPDVILTPFMLSAIISMTSINRDPESARISHSILLPFLRNVIKNNEEERTLADYSVWYRDTLQRKQVDLQQVLTVLIDQNKDGKDVITPG
LVHLVFYLLKSQSPKMHTLAITFLTKFIRKRFIFGQGIIKLISEWMIVYQEQNQFSECLTLLSVADTYTVSECMKPIQSVLEHMQWLSGEQSMRMMNFIL
PLLKISNRVRDALIDVLCKAMSSSGIQKRRMAIYGFCMILKQLNNSNAVRQTSSATSFCTQHSISGYSIMTQNTLGSRSNPQRNFDMLTLEIIGMLRNCL
QQQFDIRCSLYENLQRAVELNAKLVPHVLQVIDWHFRSFFDTPSAEDAGDDLDTVFRIRYDQLVSSSDDQHMEIQLKDNLGKLIQFVANCLAVFDRAPSG
YDTREMNRLMSFCVDRMVANRLPLEEITPPATHLKCALVLQQLNIIEGIICHLLLKSKPQNNAVCQILPLFNQHCKLLESLKALSDASKKAHKQLKQTAK
NNSTSTCNLTLNGVPVKTMCTQPDNIWDLAILDKLLHLLLDDVVAFAAPEKTVLLRSNEPLVRYVLSVTASRVESIRLEPDYKQLAYSKRTFKQLTDITK
VIYERCIQRLPELWKNFDMQSAALATQCFSECLKTAHETYSKKFEDFVKSFDLASIKGSRNMEVVYTIQDVLDDFMTEYGTDDSICKNEFVSKLPVYLLE
SLEILLDHIDYQDRAATESYTWLLNFCSKNEILNSEMGLVHRMLFVQRQKTHLGPFFDSVARQLGHVLGVQNEDAPSESVELTLKSISTVTIESCLQHLY
AAIQKQLNDVDYFVTKANNLNYKCQVVPEIDQIYWRGNLDSMDRSICTQIIHISRTLLVLTNVCIPLGSLMDGLMKLLIQHYTCLKSLTKHYISSYTSDT
SIENIKSTKFELLLRAVGKQLPANIYELITYIEANTLDEEAQQHTKKRKVQAERAKVLRETRLIPKVIMVIEDFNKHIILLSKKTKSKNRLTDYLHLGTM
RDFDIKSTDLRAAIERSISDGRNISTEDSNAEDRDEDEDENENVEEDDDAQSTTYPEDLEEKEDECLVIEKKEERRRRRAPSSEAEDPQPKRKRGRKAAE
QLEAEDEEQVEEEQPETTLKLRRNKKSGAKNQKECGH*
TRLSQDLATFPSEQLAWIVEHCMDGIRQGDAKCVGWKDLLPDALSLLLARPRFLLNGISIDGMEFRDTTVRSIATMQWPASILTPIADMFKTMNLSSGEI
VTVLNKFSGALQELSPMELPALCFQLFSMCSTASQLIITLLALEKYFHRNYYKRLFSDMCSNSTNLDSIDLFSDKELREAEETILHHLNYCTMYKLTEKH
LAIMLRNFLHMPDVILTPFMLSAIISMTSINRDPESARISHSILLPFLRNVIKNNEEERTLADYSVWYRDTLQRKQVDLQQVLTVLIDQNKDGKDVITPG
LVHLVFYLLKSQSPKMHTLAITFLTKFIRKRFIFGQGIIKLISEWMIVYQEQNQFSECLTLLSVADTYTVSECMKPIQSVLEHMQWLSGEQSMRMMNFIL
PLLKISNRVRDALIDVLCKAMSSSGIQKRRMAIYGFCMILKQLNNSNAVRQTSSATSFCTQHSISGYSIMTQNTLGSRSNPQRNFDMLTLEIIGMLRNCL
QQQFDIRCSLYENLQRAVELNAKLVPHVLQVIDWHFRSFFDTPSAEDAGDDLDTVFRIRYDQLVSSSDDQHMEIQLKDNLGKLIQFVANCLAVFDRAPSG
YDTREMNRLMSFCVDRMVANRLPLEEITPPATHLKCALVLQQLNIIEGIICHLLLKSKPQNNAVCQILPLFNQHCKLLESLKALSDASKKAHKQLKQTAK
NNSTSTCNLTLNGVPVKTMCTQPDNIWDLAILDKLLHLLLDDVVAFAAPEKTVLLRSNEPLVRYVLSVTASRVESIRLEPDYKQLAYSKRTFKQLTDITK
VIYERCIQRLPELWKNFDMQSAALATQCFSECLKTAHETYSKKFEDFVKSFDLASIKGSRNMEVVYTIQDVLDDFMTEYGTDDSICKNEFVSKLPVYLLE
SLEILLDHIDYQDRAATESYTWLLNFCSKNEILNSEMGLVHRMLFVQRQKTHLGPFFDSVARQLGHVLGVQNEDAPSESVELTLKSISTVTIESCLQHLY
AAIQKQLNDVDYFVTKANNLNYKCQVVPEIDQIYWRGNLDSMDRSICTQIIHISRTLLVLTNVCIPLGSLMDGLMKLLIQHYTCLKSLTKHYISSYTSDT
SIENIKSTKFELLLRAVGKQLPANIYELITYIEANTLDEEAQQHTKKRKVQAERAKVLRETRLIPKVIMVIEDFNKHIILLSKKTKSKNRLTDYLHLGTM
RDFDIKSTDLRAAIERSISDGRNISTEDSNAEDRDEDEDENENVEEDDDAQSTTYPEDLEEKEDECLVIEKKEERRRRRAPSSEAEDPQPKRKRGRKAAE
QLEAEDEEQVEEEQPETTLKLRRNKKSGAKNQKECGH*
Vector Type
Baculovirus
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Various silent changes, plus residue differences:
A8G
P224T
K947M.
Extra residues at C-terminus: SGAKNQKECGH*
(as in DU50185)
Recommend growth at 30 degrees.