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Plasmid
pGEX6P1 CUL2 (mouse) 1-222
Parent Plasmid
pGEX6P1
Expressed
GST CUL2 (mouse) 1-222
DU Number
DU51794
Genbank
NP_083678.2
Tag
GST
Species
Mouse
Sequence of Insert
View Sequence
GGATCCATGTCTTTAAAACCAAGAGTAGTGGATTTTGATGAGACATGGAACAAACTCCTAACTACAATCAAAGCTGTTGTTATGTTGGAATATGTCGAGC
GAGCAACATGGAATGACCGCTTCTCAGATATCTATGCTTTATGTGTGGCCTATCCTGAACCACTTGGAGAAAGACTTTATGCGGAAACAAAGATTTTTTT
GGAAAGTCACGTTCGGCATTTGTATAAGAGAGTTCTGGAGTCTGAAGAACAAGTGCTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAAGGCGCC
GACTATATGGACTGCTTATATAGGTATCTCAATACCCAGTATATTAAAAAGAATAAATTGACAGAAGCTGATATACAGTATGGCTATGGTGGAGTAGATA
TGAATGAACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGGAAGTTAATGGTTGAACCCCTTCAGAACATCCTCATCCGGATGCTGCTTCG
AGAAATTAAAAATGATCGTGGTGGTGAAGATCCAAACCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTT
CCCTTAAAGTTTTATCAGGGAATATTCGTGTCTCCCTTTCTGACTGAAACGGGAGAATATTACAAGCAAGAATAAGCGGCCGC
GAGCAACATGGAATGACCGCTTCTCAGATATCTATGCTTTATGTGTGGCCTATCCTGAACCACTTGGAGAAAGACTTTATGCGGAAACAAAGATTTTTTT
GGAAAGTCACGTTCGGCATTTGTATAAGAGAGTTCTGGAGTCTGAAGAACAAGTGCTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAAGGCGCC
GACTATATGGACTGCTTATATAGGTATCTCAATACCCAGTATATTAAAAAGAATAAATTGACAGAAGCTGATATACAGTATGGCTATGGTGGAGTAGATA
TGAATGAACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGGAAGTTAATGGTTGAACCCCTTCAGAACATCCTCATCCGGATGCTGCTTCG
AGAAATTAAAAATGATCGTGGTGGTGAAGATCCAAACCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTT
CCCTTAAAGTTTTATCAGGGAATATTCGTGTCTCCCTTTCTGACTGAAACGGGAGAATATTACAAGCAAGAATAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYAETKIFLESHV
RHLYKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQYIKKNKLTEADIQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQNILIRMLLREIKN
DRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQGIFVSPFLTETGEYYKQE*
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYAETKIFLESHV
RHLYKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQYIKKNKLTEADIQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQNILIRMLLREIKN
DRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQGIFVSPFLTETGEYYKQE*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Please can you make a construct of M. musculus Cullin2 (1-222), for expression in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP.
100042 R 1000 1001 1047 19007
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